##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062255_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	132207
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.52959374314522	32.0	21.0	32.0	14.0	32.0
2	25.39202160248701	32.0	14.0	32.0	14.0	32.0
3	25.78613840416922	32.0	14.0	32.0	14.0	32.0
4	25.43485594560046	32.0	14.0	32.0	14.0	32.0
5	25.847557239783068	32.0	21.0	32.0	14.0	32.0
6	29.084753454809505	32.0	21.0	36.0	14.0	36.0
7	28.81648475496759	32.0	21.0	36.0	14.0	36.0
8	28.612123412527325	32.0	21.0	36.0	14.0	36.0
9	28.758628514375186	32.0	21.0	36.0	14.0	36.0
10	27.58005249343832	32.0	21.0	36.0	14.0	36.0
11	29.510873100516616	36.0	21.0	36.0	14.0	36.0
12	28.323227968261893	32.0	21.0	36.0	14.0	36.0
13	28.803028583963027	32.0	21.0	36.0	14.0	36.0
14	28.201592956500033	32.0	21.0	36.0	14.0	36.0
15	28.180565325587903	32.0	21.0	36.0	14.0	36.0
16	28.299053756608956	32.0	21.0	36.0	14.0	36.0
17	28.061804594310438	32.0	21.0	36.0	14.0	36.0
18	28.114963655479663	32.0	21.0	36.0	14.0	36.0
19	28.05102604249397	32.0	21.0	36.0	14.0	36.0
20	28.025172645926464	32.0	21.0	36.0	14.0	36.0
21	28.052410235464084	32.0	21.0	36.0	14.0	36.0
22	27.756919073876574	32.0	21.0	36.0	14.0	36.0
23	27.6727102195799	32.0	14.0	36.0	14.0	36.0
24	27.60831877283351	32.0	14.0	36.0	14.0	36.0
25	26.757077915692815	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	9.0
5	12.0
6	20.0
7	6.0
8	18.0
9	7.0
10	4.0
11	2.0
12	7.0
13	8.0
14	202.0
15	464.0
16	995.0
17	1854.0
18	3181.0
19	4730.0
20	5967.0
21	6499.0
22	6620.0
23	6595.0
24	6594.0
25	6615.0
26	6678.0
27	6825.0
28	7131.0
29	7207.0
30	7528.0
31	8006.0
32	9050.0
33	9156.0
34	10998.0
35	9218.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.19472935765938	19.801972708843905	12.339263562373302	28.664034371123414
2	15.557538992178873	19.567946991815802	38.61700679243	26.257507223575328
3	17.895421573744205	23.195443509042637	27.93119880187887	30.977936115334288
4	12.038395788263415	15.65634407951468	34.95257257832711	37.352687553894796
5	14.675997730281823	35.405333837715155	33.563835823718556	16.35483260828447
6	33.72178374410703	35.78309332647239	16.194598521366032	14.300524408054544
7	29.095506298449614	30.826822916666668	20.57670179263566	19.500968992248062
8	27.277406852280812	32.32653555585836	19.410001362583458	20.986056229277377
9	25.635299728242355	14.031475439619085	19.076780996646555	41.256443835492
10	15.562991619286995	25.84015323001915	31.088887038284792	27.50796811240906
11	36.26396092833075	21.24181274372468	22.443493734146063	20.05073259379851
12	24.201400719288284	23.316297558205566	28.955517698277493	23.526784024228657
13	28.065234179802843	19.37718620814973	25.35168612486561	27.205893487181815
14	22.814879861920694	19.566533936925616	25.591606231737046	32.026979969416644
15	24.19249814497933	26.979572058088646	22.79101109983797	26.036918697094052
16	24.78595598755479	26.494523047108608	24.06679838605894	24.652722579277665
17	22.548997342901913	26.681504023497528	26.240168358579552	24.529330275021007
18	23.480294932550077	25.096519250859213	27.270662689821197	24.15252312676952
19	23.974567611550544	25.088748438860087	26.37853385308254	24.558150096506832
20	24.49271523178808	24.440491958372753	25.78694418164617	25.279848628192997
21	25.19772642987429	24.172197961052625	25.934895971300342	24.69517963777275
22	24.085723586098304	24.62092824321153	26.134187238359107	25.159160932331055
23	22.8950495349242	24.705401539404672	26.55283851387659	25.846710411794533
24	23.241749924311232	25.398122918558887	26.71813502876173	24.641992128368152
25	23.31743869209809	24.173478655767482	26.945958219800183	25.563124432334245
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	11.0
1	11.0
2	41.0
3	71.0
4	71.0
5	71.0
6	143.5
7	216.0
8	216.0
9	216.0
10	282.5
11	349.0
12	349.0
13	349.0
14	465.0
15	581.0
16	581.0
17	581.0
18	869.0
19	1157.0
20	1157.0
21	1157.0
22	1925.5
23	2694.0
24	2694.0
25	2694.0
26	3803.5
27	4913.0
28	4913.0
29	4913.0
30	5913.0
31	6913.0
32	6913.0
33	6913.0
34	7942.5
35	8972.0
36	8972.0
37	8972.0
38	9825.0
39	10678.0
40	10678.0
41	10678.0
42	11902.0
43	13126.0
44	13126.0
45	13126.0
46	14333.0
47	15540.0
48	15540.0
49	15540.0
50	16081.0
51	16622.0
52	16622.0
53	16622.0
54	15467.0
55	14312.0
56	14312.0
57	14312.0
58	13329.5
59	12347.0
60	12347.0
61	12347.0
62	10827.0
63	9307.0
64	9307.0
65	9307.0
66	7974.5
67	6642.0
68	6642.0
69	6642.0
70	5102.0
71	3562.0
72	3562.0
73	3562.0
74	2764.5
75	1967.0
76	1967.0
77	1967.0
78	1610.0
79	1253.0
80	1253.0
81	1253.0
82	889.0
83	525.0
84	525.0
85	525.0
86	384.0
87	243.0
88	243.0
89	243.0
90	156.0
91	69.0
92	69.0
93	69.0
94	46.0
95	23.0
96	23.0
97	23.0
98	68.5
99	114.0
100	114.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00226916880346729
2	7.563896011557633E-4
3	0.0
4	0.0037819480057788165
5	0.024204467236984425
6	0.043870596867034276
7	0.08395924572828974
8	0.07942090812135515
9	0.07866451852019939
10	0.08925397293638007
11	0.10740732336411839
12	0.09984342735256076
13	0.09757425854909346
14	0.0824464665259782
15	0.10059981695371653
16	0.08169007692482243
17	0.08169007692482243
18	0.0824464665259782
19	0.06958784330633022
20	0.062023947294772584
21	0.060511168092461064
22	0.08169007692482243
23	0.058998388890149536
24	0.0658058953005514
25	0.0658058953005514
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	132207.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.05453569024334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	88.38982044333113	61.92107830901541
2	7.290238293185916	10.214285174007427
3	1.765334657784208	3.710090993669019
4	0.720170163144995	2.018047455883577
5	0.4016541239729207	1.4068846581497196
6	0.24293596208039564	1.0211259615602806
7	0.16951531576276493	0.8312721716701839
8	0.12308755412073379	0.6898273162540561
9	0.0993338156062062	0.626290589756972
>10	0.6780612630510597	9.115251083528102
>50	0.08205836941382251	3.98314763968625
>100	0.03671032315881534	3.98314763968625
>500	0.0010797153870239805	0.47955100713275395
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	634	0.47955100713275395	No Hit
TATCAACGCAGAGTACTTTTTTTTT	374	0.2828897108322555	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	298	0.22540410114441747	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	225	0.17018766026004675	No Hit
GGTATCAACGCAGAGTACTTTTTTT	224	0.169431270658891	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	213	0.16111098504617757	No Hit
GTCAATCCTGTCCGTGTCCGGGCCG	205	0.15505986823693146	No Hit
ACCATACTCCCCCCGGAACCCAAAG	204	0.1543034786357757	No Hit
TCGTAGTTCCGACCATAAACGATGC	175	0.1323681802022586	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	166	0.1255606737918567	No Hit
GTATCTGATCGTCTTCGAACCTCCG	164	0.12404789458954517	No Hit
GAATAGGACCGCGGTTCTATTTTGT	162	0.12253511538723365	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	157	0.11875316738145483	No Hit
CTATTGGAGCTGGAATTACCGCGGC	154	0.11648399857798755	No Hit
GGCTCCACTCCTGGTGGTGCCCTTC	152	0.11497121937567602	No Hit
ACCATAAACGATGCCGACTGGCGAT	142	0.10740732336411839	No Hit
GAATAACGCCGCCGCATCGCCAGTC	142	0.10740732336411839	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	141	0.10665093376296263	No Hit
GAACTACGACGGTATCTGATCGTCT	140	0.10589454416180687	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	133	0.10059981695371653	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGAGG	25	0.006003559	19.0	2
CGACCCG	30	7.671752E-4	18.999998	5
CCGTCGT	30	7.671752E-4	18.999998	9
GTCGTAG	30	7.671752E-4	18.999998	11
CGTCGTA	30	7.671752E-4	18.999998	10
GGTATCA	85	3.6379788E-12	17.882353	1
CCGGGGA	35	0.0021561016	16.285715	9
TCAGATA	35	0.0021561016	16.285715	2
TCCCGAC	35	0.0021561016	16.285715	2
ACCGTCG	35	0.0021561016	16.285715	8
ATACCGT	35	0.0021561016	16.285715	6
TACCGTC	35	0.0021561016	16.285715	7
CCCGACC	35	0.0021561016	16.285715	3
CCGTCAA	75	5.707625E-8	15.200001	18
CGTCAAT	70	4.3474756E-7	14.928572	19
GACCCGG	40	0.0052453605	14.25	6
ACAGGAC	40	0.0052453605	14.25	8
TCCGTCA	80	1.2582859E-7	14.25	17
CCCCAAC	40	0.0052453605	14.25	12
CCGACCC	40	0.0052453605	14.25	4
>>END_MODULE