FastQCFastQC Report
Thu 2 Feb 2017
SRR4062255_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062255_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132207
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC2740.20725075071667914No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG2680.20271241310974458No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG2470.18682823148547353No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG2070.156572647439243No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT2040.1543034786357757No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA2020.15279069943346418No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC1850.1399320762138162No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT1840.13917568661266044No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA1740.1316117906011028No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC1630.12329150498838941No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA1580.11950955698261059No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT1520.11497121937567602No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT1510.11421482977452026No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG1400.10589454416180687No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC1400.10589454416180687No Hit
AATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCA1350.10211259615602805No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGATA301.2950711E-436.665723
ATACGAA301.2950711E-436.6657240
GGAGTGT250.002345542435.1990936
GGTATCA702.7284841E-1134.5705341
TACGAAT353.2029866E-431.4277641
CGCAATA353.2029866E-431.4277636
AATACGA353.2029866E-431.4277639
CGAATGC300.005716897529.34367443
AACCTCG454.0432213E-529.3325799
GGAATTT300.00572748429.33257738
GTCAACA300.00572748429.33257738
AGTGTGC300.00572748429.3325778
TAGATAA406.999884E-427.4992924
CTCTAGA406.999884E-427.4992921
CAATACG406.999884E-427.49929238
AGATAAC508.28785E-526.3993195
TAACCTC508.28785E-526.3993198
GGACGGC609.66946E-625.66600410
AGGGACG609.66946E-625.6660048
GTATCAA1500.024.932691
Produced by FastQC (version 0.11.5)