##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062255_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	132207
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.0820682717254	32.0	32.0	32.0	21.0	32.0
2	28.64926214194407	32.0	32.0	32.0	14.0	32.0
3	30.32371205760663	32.0	32.0	32.0	21.0	32.0
4	30.895187092967845	32.0	32.0	32.0	32.0	32.0
5	30.61487667067553	32.0	32.0	32.0	32.0	32.0
6	34.153146202546004	36.0	36.0	36.0	32.0	36.0
7	34.13380532044445	36.0	36.0	36.0	32.0	36.0
8	34.01623968473682	36.0	36.0	36.0	32.0	36.0
9	34.359171602108816	36.0	36.0	36.0	32.0	36.0
10	33.87834229655011	36.0	36.0	36.0	32.0	36.0
11	34.5835772689797	36.0	36.0	36.0	32.0	36.0
12	34.19966416301709	36.0	36.0	36.0	32.0	36.0
13	34.40737631139047	36.0	36.0	36.0	32.0	36.0
14	34.27295075147307	36.0	36.0	36.0	32.0	36.0
15	34.173394752168946	36.0	36.0	36.0	32.0	36.0
16	34.18912765587299	36.0	36.0	36.0	32.0	36.0
17	34.14032539880642	36.0	36.0	36.0	32.0	36.0
18	34.18720642628605	36.0	36.0	36.0	32.0	36.0
19	34.185126354882875	36.0	36.0	36.0	32.0	36.0
20	34.22942052992656	36.0	36.0	36.0	32.0	36.0
21	34.223497999349505	36.0	36.0	36.0	32.0	36.0
22	34.17706324173455	36.0	36.0	36.0	32.0	36.0
23	34.07868721020824	36.0	36.0	36.0	32.0	36.0
24	34.018629875876464	36.0	36.0	36.0	32.0	36.0
25	34.0096212757267	36.0	36.0	36.0	32.0	36.0
26	33.89446852284675	36.0	36.0	36.0	32.0	36.0
27	33.908386091508014	36.0	36.0	36.0	32.0	36.0
28	33.84713366160642	36.0	36.0	36.0	32.0	36.0
29	33.78116892448963	36.0	36.0	36.0	32.0	36.0
30	33.742396393534385	36.0	36.0	36.0	32.0	36.0
31	33.74210140158993	36.0	36.0	36.0	32.0	36.0
32	33.681045632984635	36.0	36.0	36.0	27.0	36.0
33	33.648067046374244	36.0	36.0	36.0	27.0	36.0
34	33.61453629535501	36.0	36.0	36.0	27.0	36.0
35	33.59074027850265	36.0	36.0	36.0	27.0	36.0
36	33.517014984077996	36.0	36.0	36.0	21.0	36.0
37	33.551884544691276	36.0	36.0	36.0	27.0	36.0
38	33.52911721769649	36.0	36.0	36.0	27.0	36.0
39	33.42475814442503	36.0	36.0	36.0	21.0	36.0
40	33.39289145052834	36.0	36.0	36.0	21.0	36.0
41	33.38590997450967	36.0	36.0	36.0	21.0	36.0
42	33.257043878160765	36.0	36.0	36.0	21.0	36.0
43	33.30980205284138	36.0	36.0	36.0	21.0	36.0
44	33.21638037320263	36.0	36.0	36.0	21.0	36.0
45	33.1513762508793	36.0	36.0	36.0	21.0	36.0
46	33.1035346086062	36.0	36.0	36.0	21.0	36.0
47	33.11677899052244	36.0	36.0	36.0	21.0	36.0
48	33.080442034082914	36.0	36.0	36.0	21.0	36.0
49	33.0242801061971	36.0	36.0	36.0	14.0	36.0
50	32.35259857647477	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	9.0
22	25.0
23	71.0
24	138.0
25	340.0
26	707.0
27	1389.0
28	2405.0
29	4275.0
30	6832.0
31	10730.0
32	16377.0
33	24913.0
34	35072.0
35	28924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.72399915272188	18.507731412836264	13.373377311102368	25.394892123339485
2	18.704709459766118	20.75989773225821	35.054689036474485	25.48070377150119
3	21.936811212719448	22.731776683534154	28.400160354595442	26.931251749150952
4	16.282798944080117	16.449204656334384	35.54804208551741	31.71995431406809
5	20.632039150725756	31.97561399925874	30.29491630549063	17.097430544524876
6	35.17514201214762	36.150128208037394	14.805570053022912	13.869159726792077
7	29.704932416589138	30.21625178697043	19.160861376477797	20.917954419962633
8	28.518157132375745	32.37952604627591	17.49453508513165	21.60778173621669
9	26.06594204542876	13.130167086462896	17.527059837981348	43.276831030127
10	15.99461450603977	25.60983911593183	29.67089488453713	28.724651493491265
11	38.66209807347569	20.125258117951393	21.08511652181806	20.127527286754862
12	24.733183568192306	22.935245486245055	27.36315021141089	24.968420734151746
13	29.359262368860957	18.486161852246855	24.17194248413473	27.982633294757463
14	23.996460096666592	18.563313591564743	24.001754823874684	33.438471487893985
15	25.985008358105095	25.905587449983734	21.067719560991474	27.041684630919693
16	26.42845256644933	26.03210141748483	22.380981196012286	25.158464820053556
17	24.15908386091508	26.444893235607797	23.54111355677082	25.8549093467063
18	25.434356728463698	24.648467932862857	25.37460194997239	24.542573388701054
19	25.813307918642735	25.140121173614105	24.52744559667794	24.519125311065224
20	26.478930767659808	24.037305135129003	23.80358074837187	25.680183348839318
21	26.91082922991975	24.09705991362031	24.065291550371764	24.92681930608818
22	25.51755958459083	24.444242740550802	24.76268276263738	25.275514912220988
23	24.280862586701158	24.17723721134282	25.402588365215156	26.13931183674087
24	24.698006883249498	25.018720925834874	24.910555576566694	25.372716614348928
25	24.759844485121704	24.029922998956174	25.26511656051919	25.94511595540293
26	24.857419481717923	24.481491006459617	25.010211336853093	25.650878174969368
27	25.046328863070787	24.597033439984266	25.240720990567823	25.115916706377124
28	24.528958375880247	24.609892063203915	25.56975046707058	25.291399093845257
29	24.977497409365615	24.826219489134463	24.904127618053508	25.292155483446415
30	24.749824139417733	24.71805577616919	25.586391038296007	24.945729046117073
31	25.038008577458076	25.09019945993782	24.731670788989994	25.140121173614105
32	25.82314098345776	24.945729046117073	24.18177554894975	25.04935442147541
33	24.454832194966983	24.667377672891753	25.33526969071229	25.542520441428973
34	25.108542728771766	24.605539839341635	25.398998532593076	24.886918899293526
35	26.332947574636744	24.42684577972422	24.910935124463908	24.329271521175126
36	23.986627031851565	25.093225018342448	24.920768189278935	25.999379760527052
37	25.816162899760975	24.528758585216785	24.9992435932347	24.65583492178754
38	25.16659480965456	24.168160536128948	24.947998214920542	25.717246439295955
39	25.12877532959677	24.810335307510194	24.21959502900754	25.841294333885497
40	25.760360646561832	25.53798210382204	24.424576610920752	24.277080638695377
41	24.647141582076458	24.942892153154926	25.467830506936146	24.942135757832474
42	25.933238023918125	25.756991248175126	24.609496146020078	23.700274581886674
43	25.1036308623298	24.373676248108925	25.379727685325264	25.142965204236006
44	25.123678119184863	25.227310549327527	24.37480143421232	25.274209897275302
45	25.076397083295515	26.09452058939215	24.384284893044082	24.444797434268253
46	24.348733775074884	25.360049620283803	24.650540074430427	25.640676530210886
47	24.78026715882789	24.96860959411827	25.41185725307475	24.839265993979094
48	26.143162958872608	25.82092148957254	23.770981626184767	24.264933925370087
49	24.51571423168564	25.846980068832494	24.52252184108014	25.114783858401722
50	26.015657501607354	25.135206686585228	24.312242350894444	24.536893460912975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2.0
1	2.0
2	2.0
3	5.0
4	8.0
5	12.5
6	17.0
7	12.5
8	8.0
9	13.5
10	19.0
11	31.0
12	43.0
13	80.5
14	118.0
15	166.0
16	214.0
17	262.5
18	311.0
19	349.0
20	387.0
21	491.5
22	596.0
23	712.5
24	829.0
25	1049.5
26	1270.0
27	1589.0
28	1908.0
29	2073.5
30	2239.0
31	2408.5
32	2578.0
33	2831.0
34	3084.0
35	3446.5
36	3809.0
37	4193.5
38	4578.0
39	4995.5
40	5413.0
41	5859.0
42	6305.0
43	6236.0
44	6167.0
45	6574.0
46	6981.0
47	7460.0
48	7939.0
49	8184.0
50	8429.0
51	8224.0
52	8019.0
53	8050.0
54	8081.0
55	8862.5
56	9644.0
57	9440.0
58	9236.0
59	8498.0
60	7760.0
61	7196.0
62	6632.0
63	5791.5
64	4951.0
65	4337.5
66	3724.0
67	3343.0
68	2962.0
69	2834.0
70	2706.0
71	2105.5
72	1505.0
73	1359.5
74	1214.0
75	986.0
76	758.0
77	631.0
78	504.0
79	485.0
80	466.0
81	385.0
82	304.0
83	262.0
84	220.0
85	159.0
86	98.0
87	79.0
88	60.0
89	44.5
90	29.0
91	21.5
92	14.0
93	14.0
94	14.0
95	10.5
96	7.0
97	10.0
98	13.0
99	22.5
100	32.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014371402421959502
2	0.0037819480057788165
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.563896011557633E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0015127792023115266
25	7.563896011557633E-4
26	7.563896011557633E-4
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	7.563896011557633E-4
35	0.0
36	0.0
37	0.00226916880346729
38	0.0
39	0.0
40	0.0
41	7.563896011557633E-4
42	0.00453833760693458
43	0.0052947272080903435
44	0.00680750641040187
45	0.00226916880346729
46	0.00226916880346729
47	7.563896011557633E-4
48	0.006051116809246106
49	0.0015127792023115266
50	0.0015127792023115266
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	132207.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.04967564494513
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.17499999815148	68.57779501142348
2	6.328999999870261	9.62636794293982
3	1.443999999970399	3.2944719488714886
4	0.6150000020288744	1.8708220270374598
5	0.33400000000154806	1.27002958327647
6	0.19699999999599907	0.8989071661049954
7	0.1409999999971098	0.7506102986002225
8	0.09199999999811406	0.5597256127353222
9	0.09199999999811406	0.6296913143272375
>10	0.5149999999894428	7.480246096283463
>50	0.042999999999118535	2.165894762323638
>100	0.022999999999528516	2.875438236076431
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	274	0.20725075071667914	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	268	0.20271241310974458	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	247	0.18682823148547353	No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG	207	0.156572647439243	No Hit
GTATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATAT	204	0.1543034786357757	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	202	0.15279069943346418	No Hit
TTCGTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGAC	185	0.1399320762138162	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	184	0.13917568661266044	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	174	0.1316117906011028	No Hit
CTCCCGACCCGGGGAGGTAGTGACGAAAAATAACAATACAGGACTCTTTC	163	0.12329150498838941	No Hit
AAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGA	158	0.11950955698261059	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	152	0.11497121937567602	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	151	0.11421482977452026	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	140	0.10589454416180687	No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC	140	0.10589454416180687	No Hit
AATACATGCCGACGGGCGCTGACCCCCCTTCCCGGGGGGGGATGCGTGCA	135	0.10211259615602805	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0015127792023115266
13	0.0	0.0	0.0	0.0	0.0015127792023115266
14	0.0	0.0	0.0	0.0	0.0015127792023115266
15	0.0	0.0	0.0	0.0	0.0015127792023115266
16	0.0	0.0	0.0	0.0	0.0015127792023115266
17	0.0	0.0	0.0	0.0	0.0015127792023115266
18	0.0	0.0	0.0	7.563896011557633E-4	0.0015127792023115266
19	0.0	0.0	0.0	7.563896011557633E-4	0.0015127792023115266
20	0.0	0.0	0.0	7.563896011557633E-4	0.0015127792023115266
21	0.0	0.0	0.0	7.563896011557633E-4	0.00226916880346729
22	0.0	0.0	0.0	7.563896011557633E-4	0.00226916880346729
23	0.0	0.0	0.0	0.0015127792023115266	0.00226916880346729
24	0.0	0.0	0.0	0.0015127792023115266	0.00226916880346729
25	0.0	0.0	0.0	0.0015127792023115266	0.00226916880346729
26	0.0	0.0	0.0	0.0015127792023115266	0.00226916880346729
27	0.0	0.0	0.0	0.0015127792023115266	0.00226916880346729
28	0.0	0.0	0.0	0.0015127792023115266	0.00226916880346729
29	0.0	0.0	0.0	0.0015127792023115266	0.00226916880346729
30	0.0	0.0	0.0	0.0015127792023115266	0.00226916880346729
31	0.0	0.0	0.0	0.0015127792023115266	0.00226916880346729
32	0.0	0.0	0.0	0.00226916880346729	0.003025558404623053
33	0.0	0.0	0.0	0.0037819480057788165	0.003025558404623053
34	0.0	0.0	0.0	0.00680750641040187	0.003025558404623053
35	0.0	0.0	0.0	0.00680750641040187	0.003025558404623053
36	0.0	0.0	0.0	0.008320285612713396	0.003025558404623053
37	0.0	0.0	0.0	0.010589454416180687	0.003025558404623053
38	0.0	0.0	0.0	0.01664057122542679	0.003025558404623053
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGATA	30	1.2950711E-4	36.66572	3
ATACGAA	30	1.2950711E-4	36.66572	40
GGAGTGT	25	0.0023455424	35.199093	6
GGTATCA	70	2.7284841E-11	34.570534	1
TACGAAT	35	3.2029866E-4	31.42776	41
CGCAATA	35	3.2029866E-4	31.42776	36
AATACGA	35	3.2029866E-4	31.42776	39
CGAATGC	30	0.0057168975	29.343674	43
AACCTCG	45	4.0432213E-5	29.332579	9
GGAATTT	30	0.005727484	29.332577	38
GTCAACA	30	0.005727484	29.332577	38
AGTGTGC	30	0.005727484	29.332577	8
TAGATAA	40	6.999884E-4	27.499292	4
CTCTAGA	40	6.999884E-4	27.499292	1
CAATACG	40	6.999884E-4	27.499292	38
AGATAAC	50	8.28785E-5	26.399319	5
TAACCTC	50	8.28785E-5	26.399319	8
GGACGGC	60	9.66946E-6	25.666004	10
AGGGACG	60	9.66946E-6	25.666004	8
GTATCAA	150	0.0	24.93269	1
>>END_MODULE