FastQCFastQC Report
Thu 2 Feb 2017
SRR4062254_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062254_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510105
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT22890.15157886372139687No Hit
GCGCAAGACGGACCAGAGCGAAAGC16670.11038967489015665No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATTA400.00527277414.25253710
GGTATCA7000.013.5648461
AGGATCG500.001498999113.3006045
CCGTCAA2500.012.91758818
CGCGGTC905.39294E-712.66892310
GAACCGC1155.307811E-912.3935116
TAAGACT1405.2750693E-1112.213224
GTTAGAC701.0927627E-412.2120053
GCGAATT550.00306428412.0930619
GTATCAA16850.012.0030781
AGAACCG1353.7107384E-1011.9635075
CGGTCCA3000.011.71875310
GATAACG907.445262E-611.61317911
CGTCGTA2950.011.59528410
CGACCAT3650.011.45409410
AACCGCG1001.924367E-611.4024087
ACGGTAT2500.011.402039
CGCATCG2250.011.40089613
CGTCAAT2800.011.19396719
CGCGTAA1451.2132659E-911.13991410
Produced by FastQC (version 0.11.5)