##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062254_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1510105 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.231109095062926 32.0 32.0 32.0 32.0 32.0 2 30.73582101906821 32.0 32.0 32.0 32.0 32.0 3 30.761145085937734 32.0 32.0 32.0 32.0 32.0 4 30.766511600186742 32.0 32.0 32.0 32.0 32.0 5 30.677844255863004 32.0 32.0 32.0 32.0 32.0 6 34.3363838938352 36.0 36.0 36.0 32.0 36.0 7 34.2655735859427 36.0 36.0 36.0 32.0 36.0 8 34.21424205601597 36.0 36.0 36.0 32.0 36.0 9 34.36451372586674 36.0 36.0 36.0 32.0 36.0 10 34.02932908638803 36.0 36.0 36.0 32.0 36.0 11 34.35418000735048 36.0 36.0 36.0 32.0 36.0 12 34.152260273292256 36.0 36.0 36.0 32.0 36.0 13 34.258272106906475 36.0 36.0 36.0 32.0 36.0 14 34.15058356869224 36.0 36.0 36.0 32.0 36.0 15 34.09677009214591 36.0 36.0 36.0 32.0 36.0 16 34.093515351581516 36.0 36.0 36.0 32.0 36.0 17 34.035329993609714 36.0 36.0 36.0 32.0 36.0 18 34.034831352786725 36.0 36.0 36.0 32.0 36.0 19 34.03961976153976 36.0 36.0 36.0 32.0 36.0 20 34.03336787839256 36.0 36.0 36.0 32.0 36.0 21 34.01160912651769 36.0 36.0 36.0 32.0 36.0 22 33.9778518712275 36.0 36.0 36.0 32.0 36.0 23 33.946190496687315 36.0 36.0 36.0 32.0 36.0 24 33.91715013194447 36.0 36.0 36.0 32.0 36.0 25 33.56590170882157 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 27.0 5 114.0 6 320.0 7 72.0 8 237.0 9 188.0 10 105.0 11 33.0 12 65.0 13 61.0 14 242.0 15 352.0 16 574.0 17 693.0 18 1005.0 19 1290.0 20 1991.0 21 2961.0 22 4568.0 23 6579.0 24 9391.0 25 13404.0 26 18475.0 27 23768.0 28 31370.0 29 41324.0 30 54580.0 31 74269.0 32 104910.0 33 147632.0 34 317105.0 35 652400.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.184356683612684 17.408775244373015 11.625407509093835 25.781460562920465 2 16.961050949572247 19.354334577620964 37.14115810796761 26.543456364839173 3 19.332596954357747 22.659480349541617 28.109744306969375 29.898178389131257 4 13.105198952553648 15.545200594983436 34.85156540777955 36.49803504468336 5 15.376931290954232 35.871932655543276 33.077174797232665 15.673961256269822 6 35.005201534618365 34.41023874049974 16.54684362927967 14.037716095602217 7 31.03072486825097 29.932982002585263 19.94391965794969 19.09237347121408 8 28.23155610149638 32.90544889748605 19.003822985243023 19.859172015774536 9 26.94861869186264 14.799299119789392 18.7254746620401 39.52660752630786 10 16.849603360889873 26.32796524534337 30.54048212007649 26.28194927369027 11 37.07110118510817 21.39023426310038 21.808987512333825 19.72967703945763 12 24.568108669388796 23.620314905704664 28.252462977427733 23.559113447478804 13 29.333960117435826 19.421267871966307 24.96936802658484 26.27540398401303 14 23.605314954757386 19.654964157931825 24.79032738164295 31.94939350566784 15 25.344960885646046 26.953231194410076 21.85570059052795 25.84610732941593 16 25.994627255935942 25.81098336332187 23.228565708049967 24.96582367269222 17 24.253998773433228 25.979546170293876 24.793489250965507 24.972965805307386 18 24.86646880338679 25.199393549268155 25.541798231163266 24.392339416181787 19 25.536977811655927 25.09334349677697 25.091222430278226 24.27845626128888 20 25.79931904029354 24.44681137336595 24.61628908111533 25.137580505225177 21 26.55727534890935 24.32645600100214 24.398302461669182 24.717966188419332 22 25.933197929703244 24.34746409859364 24.718873957957705 25.00046401374541 23 24.543258477230026 24.319982503752748 25.254374890234242 25.88238412878298 24 24.658033608744585 24.956023897548608 25.076784196970518 25.30915829673629 25 24.946082622498825 24.382253328826412 25.06153937923767 25.610124669437102 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 469.0 1 469.0 2 499.5 3 530.0 4 530.0 5 530.0 6 1030.5 7 1531.0 8 1531.0 9 1531.0 10 1831.5 11 2132.0 12 2132.0 13 2132.0 14 3004.5 15 3877.0 16 3877.0 17 3877.0 18 6431.5 19 8986.0 20 8986.0 21 8986.0 22 14397.5 23 19809.0 24 19809.0 25 19809.0 26 29131.5 27 38454.0 28 38454.0 29 38454.0 30 48371.5 31 58289.0 32 58289.0 33 58289.0 34 73725.5 35 89162.0 36 89162.0 37 89162.0 38 105098.0 39 121034.0 40 121034.0 41 121034.0 42 138848.0 43 156662.0 44 156662.0 45 156662.0 46 174883.5 47 193105.0 48 193105.0 49 193105.0 50 200784.0 51 208463.0 52 208463.0 53 208463.0 54 196259.0 55 184055.0 56 184055.0 57 184055.0 58 169361.5 59 154668.0 60 154668.0 61 154668.0 62 136087.0 63 117506.0 64 117506.0 65 117506.0 66 95775.0 67 74044.0 68 74044.0 69 74044.0 70 56054.0 71 38064.0 72 38064.0 73 38064.0 74 28657.0 75 19250.0 76 19250.0 77 19250.0 78 15533.5 79 11817.0 80 11817.0 81 11817.0 82 8176.5 83 4536.0 84 4536.0 85 4536.0 86 3354.5 87 2173.0 88 2173.0 89 2173.0 90 1450.0 91 727.0 92 727.0 93 727.0 94 453.0 95 179.0 96 179.0 97 179.0 98 381.0 99 583.0 100 583.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0010595289731508736 2 2.648822432877184E-4 3 8.608672906850849E-4 4 0.009734422440823651 5 0.03264673648521129 6 0.06191622436850418 7 0.10297297207810052 8 0.1058204561934435 9 0.11363448237043118 10 0.1280043440687899 11 0.13621569361070918 12 0.13052072538002324 13 0.12429599266276187 14 0.11138298330248558 15 0.1299909608934478 16 0.11595220199919873 17 0.12118362630413115 18 0.10966124872111543 19 0.09469540197535932 20 0.08953019823124882 21 0.08820578701481023 22 0.10125123749673036 23 0.08012687859453481 24 0.0881395664539883 25 0.08708003748083742 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1510105.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.50072719445998 #Duplication Level Percentage of deduplicated Percentage of total 1 79.40913039240381 44.866736130505075 2 12.460461523150265 14.080502744731566 3 3.5159888227538216 5.959677758795524 4 1.4266956891479052 3.224373756882314 5 0.803881750594492 2.2709951743472154 6 0.484716193446587 1.6432090447597623 7 0.33679437626066766 1.3320389021612602 8 0.23845444317328024 1.077827955363229 9 0.17796580551705754 0.9049677684715429 >10 0.9967635901490496 10.166172266689353 >50 0.07566273989100851 2.9344644832296707 >100 0.06487421732100311 7.53756256894895 >500 0.006723232916427097 2.587031294703868 >1k 0.0018872232747865537 1.4144401504107498 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2289 0.15157886372139687 No Hit GCGCAAGACGGACCAGAGCGAAAGC 1667 0.11038967489015665 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.324411216438592E-4 2 0.0 0.0 0.0 0.0 1.324411216438592E-4 3 0.0 0.0 0.0 0.0 1.324411216438592E-4 4 0.0 0.0 0.0 0.0 1.324411216438592E-4 5 0.0 0.0 0.0 0.0 1.324411216438592E-4 6 0.0 0.0 0.0 0.0 1.324411216438592E-4 7 0.0 6.62205608219296E-5 0.0 0.0 1.324411216438592E-4 8 0.0 6.62205608219296E-5 0.0 0.0 1.324411216438592E-4 9 0.0 6.62205608219296E-5 0.0 0.0 1.324411216438592E-4 10 0.0 6.62205608219296E-5 0.0 0.0 1.986616824657888E-4 11 0.0 6.62205608219296E-5 0.0 0.0 1.986616824657888E-4 12 0.0 6.62205608219296E-5 0.0 1.324411216438592E-4 6.62205608219296E-4 13 0.0 6.62205608219296E-5 0.0 1.324411216438592E-4 7.946467298631552E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATTA 40 0.005272774 14.252537 10 GGTATCA 700 0.0 13.564846 1 AGGATCG 50 0.0014989991 13.300604 5 CCGTCAA 250 0.0 12.917588 18 CGCGGTC 90 5.39294E-7 12.668923 10 GAACCGC 115 5.307811E-9 12.393511 6 TAAGACT 140 5.2750693E-11 12.21322 4 GTTAGAC 70 1.0927627E-4 12.212005 3 GCGAATT 55 0.003064284 12.093061 9 GTATCAA 1685 0.0 12.003078 1 AGAACCG 135 3.7107384E-10 11.963507 5 CGGTCCA 300 0.0 11.718753 10 GATAACG 90 7.445262E-6 11.613179 11 CGTCGTA 295 0.0 11.595284 10 CGACCAT 365 0.0 11.454094 10 AACCGCG 100 1.924367E-6 11.402408 7 ACGGTAT 250 0.0 11.40203 9 CGCATCG 225 0.0 11.400896 13 CGTCAAT 280 0.0 11.193967 19 CGCGTAA 145 1.2132659E-9 11.139914 10 >>END_MODULE