FastQCFastQC Report
Thu 2 Feb 2017
SRR4062254_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062254_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1510105
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT15780.1044960449770049No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGATA300.0057420829.33686439
GTATCAA18550.024.9103091
GGTATCA8500.023.8162081
ACCGTCG3350.019.7006038
CGTCGTA3600.018.94421810
TACCGTC3500.018.8562937
GTCGTAG3850.018.28549411
ATACCGT3750.018.1858466
CCGTCGT4050.017.9251179
GTATTAG3100.017.7452471
CGCAATA3600.017.72318336
GTCGATA750.001290603517.60095436
CGTTAAG750.001291176717.59978731
TCAACGC27550.016.7687514
CGAGCCG4400.016.49980215
CTACCGT800.0019892616.4992544
ATCAACG28450.016.3156073
CAACGCA28300.016.2466165
CTAGCGG3700.016.0538629
AACGCAG29100.015.79997356
Produced by FastQC (version 0.11.5)