##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062254_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1510105
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.277384685170897	32.0	32.0	32.0	32.0	32.0
2	31.427372268815745	32.0	32.0	32.0	32.0	32.0
3	31.560207402796493	32.0	32.0	32.0	32.0	32.0
4	31.631650117044842	32.0	32.0	32.0	32.0	32.0
5	31.612313713284838	32.0	32.0	32.0	32.0	32.0
6	35.23725502531281	36.0	36.0	36.0	36.0	36.0
7	35.24489621582605	36.0	36.0	36.0	36.0	36.0
8	35.19665255065045	36.0	36.0	36.0	36.0	36.0
9	35.28042619552945	36.0	36.0	36.0	36.0	36.0
10	35.1579035894855	36.0	36.0	36.0	36.0	36.0
11	35.280088470669256	36.0	36.0	36.0	36.0	36.0
12	35.1979617311379	36.0	36.0	36.0	36.0	36.0
13	35.23873571705279	36.0	36.0	36.0	36.0	36.0
14	35.19776306945543	36.0	36.0	36.0	36.0	36.0
15	35.17554342247725	36.0	36.0	36.0	36.0	36.0
16	35.18897626323997	36.0	36.0	36.0	36.0	36.0
17	35.16736120998209	36.0	36.0	36.0	36.0	36.0
18	35.161640415732684	36.0	36.0	36.0	36.0	36.0
19	35.15391777392963	36.0	36.0	36.0	36.0	36.0
20	35.161865565639474	36.0	36.0	36.0	36.0	36.0
21	35.150063075084184	36.0	36.0	36.0	36.0	36.0
22	35.13378473682294	36.0	36.0	36.0	36.0	36.0
23	35.100055294168286	36.0	36.0	36.0	36.0	36.0
24	35.08417096824393	36.0	36.0	36.0	36.0	36.0
25	35.07308961959599	36.0	36.0	36.0	36.0	36.0
26	35.02456253042007	36.0	36.0	36.0	36.0	36.0
27	35.0183291890299	36.0	36.0	36.0	36.0	36.0
28	34.99428715221789	36.0	36.0	36.0	36.0	36.0
29	34.97826045208777	36.0	36.0	36.0	36.0	36.0
30	34.9609974140871	36.0	36.0	36.0	36.0	36.0
31	34.96024183748812	36.0	36.0	36.0	36.0	36.0
32	34.93850361398711	36.0	36.0	36.0	36.0	36.0
33	34.90851828184133	36.0	36.0	36.0	32.0	36.0
34	34.89787465110042	36.0	36.0	36.0	32.0	36.0
35	34.8878097880611	36.0	36.0	36.0	32.0	36.0
36	34.86725227715954	36.0	36.0	36.0	32.0	36.0
37	34.85724105277448	36.0	36.0	36.0	32.0	36.0
38	34.841269977915445	36.0	36.0	36.0	32.0	36.0
39	34.82599554335626	36.0	36.0	36.0	32.0	36.0
40	34.81390896659504	36.0	36.0	36.0	32.0	36.0
41	34.79158601554197	36.0	36.0	36.0	32.0	36.0
42	34.757636720625385	36.0	36.0	36.0	32.0	36.0
43	34.75723277520437	36.0	36.0	36.0	32.0	36.0
44	34.7116664073028	36.0	36.0	36.0	32.0	36.0
45	34.69885537760619	36.0	36.0	36.0	32.0	36.0
46	34.68639134364829	36.0	36.0	36.0	32.0	36.0
47	34.65747746017661	36.0	36.0	36.0	32.0	36.0
48	34.62957211584625	36.0	36.0	36.0	32.0	36.0
49	34.62033832084524	36.0	36.0	36.0	32.0	36.0
50	34.20824777085037	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	13.0
21	38.0
22	113.0
23	295.0
24	805.0
25	1744.0
26	3655.0
27	6851.0
28	12067.0
29	19184.0
30	28991.0
31	42580.0
32	64556.0
33	109175.0
34	246969.0
35	973065.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75995262484334	17.889553354018012	12.029196132618642	26.32129788852
2	16.280706866351665	19.78907913340331	36.982474032230826	26.9477399680142
3	18.607840540361565	23.05052645520164	28.439904642076684	29.901728362360107
4	12.805872972987292	15.86314584955738	35.089437548672144	36.241543628783184
5	14.949159164428963	36.38780084828539	32.83228649663434	15.830753490651311
6	35.149460300642346	34.64247400834382	16.376663797099532	13.831401893914311
7	31.031683227325253	29.995463891583697	19.9217935176693	19.05105936342175
8	28.29637674201463	32.932610646279564	18.989474241857355	19.78153836984845
9	27.134868105197985	14.461842057340386	18.602481284413997	39.80080855304763
10	16.82334461732437	26.458839920959086	30.523791738847127	26.19402372286942
11	37.26720989600061	21.241370633167893	21.808417295486077	19.683002175345425
12	24.634594703411157	23.493547464280606	28.23090330202423	23.640954530284006
13	29.395108287172082	19.30984931511385	25.007863691597603	26.28717870611646
14	23.74517003784505	19.426331281599623	24.76337738104304	32.065121299512285
15	25.41101446588151	26.87011830303191	21.881591015194306	25.837276215892274
16	26.098438644379375	25.687339497171706	23.28782635299166	24.92639550545726
17	24.330758457193376	25.909522847749	24.74821287261482	25.01150582244281
18	25.095622553148655	24.954109120961203	25.579430290893875	24.370838034996265
19	25.71503306061499	24.905751586810187	25.13500716837571	24.244208184199113
20	25.882129348067938	24.328651953253303	24.705780294762718	25.08343840391604
21	26.69798020399933	24.112593644274597	24.471708221227292	24.717717930498782
22	26.008588806738604	24.123223219577447	24.88482588959046	24.983362084093493
23	24.62669814350658	24.18864913366951	25.33267554242917	25.85197718039474
24	24.819579812010705	24.73713417646412	25.183201486537854	25.26008452498732
25	25.130719819879477	24.241109860274154	25.231242964042117	25.396927355804248
26	24.82966326004224	25.067594995553264	25.355457298438445	24.747284445966052
27	25.306651674291984	24.689408191902395	24.816022239572057	25.187917894233564
28	25.01983330750699	24.503107770021046	25.334186264888224	25.14287265758374
29	24.840093265408427	24.799566121161593	25.315625002069403	25.04471561136058
30	24.907968634836628	24.748904530413228	25.435886636694804	24.907240198055337
31	25.293722659059213	24.630520668195043	24.648201639893568	25.427555032852172
32	25.338273419884178	24.62626523496866	24.585671762372566	25.449789582774596
33	24.864611794435078	24.435766590601165	25.06128725079498	25.63833436416878
34	25.452821292533944	24.519034652285164	25.25296539009647	24.775178665084418
35	25.900121912375397	24.272879761022605	25.2670516740271	24.559946652574894
36	24.828604179455546	24.931709865763946	24.95799949804682	25.281686456733688
37	25.709468542601222	24.667996402825292	24.794149160902048	24.828385893671438
38	24.983825628019243	24.35876975442105	25.24784700401628	25.409557613543427
39	25.615573039347804	24.421477560712987	24.58835425803773	25.37459514190148
40	25.76747974478596	24.593389201413146	24.951973538263896	24.687157515537
41	24.737879687885947	24.998394134385457	25.333142616097458	24.93058356163114
42	25.904354091447313	24.906690471964758	24.836493098141226	24.352462338446703
43	25.39871750807435	24.103510594829636	25.155013579209406	25.34275831788661
44	24.984719018679552	24.772209076936015	24.8328032173833	25.410268687001132
45	25.191432156893907	24.84230811256469	24.864360143437533	25.101899587103865
46	25.112660564067685	24.752676536868893	24.84479165493981	25.289871244123606
47	25.155520119939556	24.619063449030325	25.210947133092375	25.014469297937747
48	25.842075323383547	25.14514429664203	24.321396193103666	24.691384186870756
49	25.127330305175526	25.213751341843878	24.429339710168346	25.22957864281225
50	25.22710687234203	25.297897730057045	24.60700809624908	24.867987301351846
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	242.0
1	143.5
2	45.0
3	157.0
4	269.0
5	262.0
6	255.0
7	204.0
8	153.0
9	214.0
10	275.0
11	461.5
12	648.0
13	1266.5
14	1885.0
15	2266.5
16	2648.0
17	2654.0
18	2660.0
19	2593.0
20	2526.0
21	2980.0
22	3434.0
23	4196.0
24	4958.0
25	6411.5
26	7865.0
27	10004.0
28	12143.0
29	14491.0
30	16839.0
31	19275.0
32	21711.0
33	25110.0
34	28509.0
35	33184.5
36	37860.0
37	44907.5
38	51955.0
39	57937.0
40	63919.0
41	70007.0
42	76095.0
43	79834.5
44	83574.0
45	91025.5
46	98477.0
47	105156.5
48	111836.0
49	116391.0
50	120946.0
51	118091.5
52	115237.0
53	114706.5
54	114176.0
55	114482.0
56	114788.0
57	110549.5
58	106311.0
59	97108.0
60	87905.0
61	77178.0
62	66451.0
63	57471.5
64	48492.0
65	40735.5
66	32979.0
67	28290.0
68	23601.0
69	21135.5
70	18670.0
71	14628.5
72	10587.0
73	9252.5
74	7918.0
75	5828.0
76	3738.0
77	3354.5
78	2971.0
79	2463.5
80	1956.0
81	1522.5
82	1089.0
83	961.5
84	834.0
85	629.5
86	425.0
87	279.0
88	133.0
89	91.5
90	50.0
91	34.5
92	19.0
93	18.5
94	18.0
95	17.5
96	17.0
97	13.0
98	9.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02999791405233411
2	0.003973233649315776
3	3.31102804109648E-4
4	6.62205608219296E-5
5	0.0
6	3.31102804109648E-4
7	0.0
8	0.0
9	0.0
10	6.62205608219296E-5
11	0.0
12	6.62205608219296E-5
13	0.0
14	0.0
15	0.0
16	4.635439257535072E-4
17	0.0
18	6.62205608219296E-5
19	0.0
20	5.959850473973665E-4
21	1.324411216438592E-4
22	0.0
23	0.0
24	0.0012581906556166625
25	3.31102804109648E-4
26	5.297644865754368E-4
27	2.648822432877184E-4
28	0.0012581906556166625
29	3.973233649315776E-4
30	0.0014568523380824514
31	4.635439257535072E-4
32	6.62205608219296E-4
33	1.986616824657888E-4
34	0.0011257495339728031
35	2.648822432877184E-4
36	2.648822432877184E-4
37	0.002052837385479818
38	0.0
39	5.297644865754368E-4
40	0.0
41	0.0010595289731508736
42	0.005098983183288579
43	0.00503276262246665
44	0.003840792527671917
45	0.002648822432877184
46	0.002516381311233325
47	7.284261690412257E-4
48	0.0037083514060280575
49	0.0037083514060280575
50	0.0015230728989043807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1510105.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.84993570643682
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.10639014234899	45.76299583703196
2	12.845365750778612	14.862071656164161
3	3.555756969129996	6.171009361556547
4	1.4696561456881558	3.4007805415451813
5	0.7651106726216361	2.213080160973514
6	0.4662430722701809	1.6183279052640924
7	0.31354431622221574	1.2696962974201709
8	0.24219126559975546	1.1208599314885133
9	0.16291107092075918	0.8481955480769416
>10	0.9391407636161605	9.772480490208793
>50	0.07074001645313237	2.845559086934436
>100	0.05522531309553227	6.590470005083899
>500	0.0058798442387902935	2.227973403215123
>1k	0.0018446570160910725	1.2964997750366345
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1578	0.1044960449770049	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.31102804109648E-4
2	0.0	0.0	0.0	0.0	3.31102804109648E-4
3	0.0	0.0	0.0	0.0	3.31102804109648E-4
4	0.0	0.0	0.0	0.0	3.973233649315776E-4
5	0.0	0.0	0.0	0.0	3.973233649315776E-4
6	0.0	0.0	0.0	0.0	3.973233649315776E-4
7	0.0	0.0	0.0	0.0	3.973233649315776E-4
8	0.0	0.0	0.0	0.0	3.973233649315776E-4
9	0.0	0.0	0.0	0.0	3.973233649315776E-4
10	0.0	0.0	0.0	0.0	3.973233649315776E-4
11	0.0	0.0	0.0	0.0	3.973233649315776E-4
12	0.0	0.0	0.0	6.62205608219296E-5	7.284261690412256E-4
13	0.0	0.0	0.0	6.62205608219296E-5	8.608672906850848E-4
14	0.0	0.0	0.0	1.986616824657888E-4	8.608672906850848E-4
15	0.0	0.0	0.0	3.973233649315776E-4	0.0010595289731508736
16	0.0	0.0	0.0	5.297644865754368E-4	0.001191970094794733
17	0.0	0.0	0.0	5.297644865754368E-4	0.001191970094794733
18	0.0	0.0	0.0	5.959850473973665E-4	0.001324411216438592
19	0.0	0.0	0.0	7.284261690412256E-4	0.001324411216438592
20	0.0	0.0	0.0	7.946467298631552E-4	0.0015892934597263105
21	0.0	0.0	0.0	9.93308412328944E-4	0.0015892934597263105
22	0.0	0.0	0.0	0.001191970094794733	0.0015892934597263105
23	0.0	0.0	0.0	0.00165551402054824	0.0015892934597263105
24	0.0	0.0	0.0	0.002185278507123677	0.0015892934597263105
25	0.0	0.0	0.0	0.0027150429936991134	0.0015892934597263105
26	0.0	0.0	0.0	0.003509689723562269	0.00165551402054824
27	0.0	0.0	0.0	0.0040394542101377055	0.00165551402054824
28	0.0	0.0	0.0	0.004767880379178932	0.0017217345813701696
29	0.0	0.0	0.0	0.006555835521371031	0.0017879551421920992
30	0.0	0.0	0.0	0.009734422440823651	0.0017879551421920992
31	0.0	0.0	0.0	0.01675380188794819	0.0017879551421920992
32	0.0	0.0	0.0	0.022779872922743784	0.0017879551421920992
33	0.0	0.0	0.0	0.02986547293069025	0.0017879551421920992
34	0.0	0.0	6.62205608219296E-5	0.03609020564795163	0.001986616824657888
35	0.0	6.62205608219296E-5	6.62205608219296E-5	0.044102893507405115	0.001986616824657888
36	0.0	6.62205608219296E-5	6.62205608219296E-5	0.05555905052959893	0.001986616824657888
37	0.0	6.62205608219296E-5	6.62205608219296E-5	0.07218041129590326	0.001986616824657888
38	0.0	6.62205608219296E-5	6.62205608219296E-5	0.09317232907645495	0.002119057946301747
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCGATA	30	0.00574208	29.336864	39
GTATCAA	1855	0.0	24.910309	1
GGTATCA	850	0.0	23.816208	1
ACCGTCG	335	0.0	19.700603	8
CGTCGTA	360	0.0	18.944218	10
TACCGTC	350	0.0	18.856293	7
GTCGTAG	385	0.0	18.285494	11
ATACCGT	375	0.0	18.185846	6
CCGTCGT	405	0.0	17.925117	9
GTATTAG	310	0.0	17.745247	1
CGCAATA	360	0.0	17.723183	36
GTCGATA	75	0.0012906035	17.600954	36
CGTTAAG	75	0.0012911767	17.599787	31
TCAACGC	2755	0.0	16.768751	4
CGAGCCG	440	0.0	16.499802	15
CTACCGT	80	0.00198926	16.499254	4
ATCAACG	2845	0.0	16.315607	3
CAACGCA	2830	0.0	16.246616	5
CTAGCGG	370	0.0	16.05386	29
AACGCAG	2910	0.0	15.7999735	6
>>END_MODULE