FastQCFastQC Report
Thu 2 Feb 2017
SRR4062253_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062253_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111999
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT3810.34018160876436393No Hit
GTATAGGGGTCCTAGGAAGATAATA3260.29107402744667366No Hit
TCCTAACACTCCTCGTCCCCATTCT3210.28660970187233814No Hit
CCCCTATACTATATCAATTTACCAG2640.23571639032491362No Hit
TATCAACGCAGAGTACTTTTTTTTT2460.2196448182573059No Hit
GTATAGTAGGGGTGAAATGGAATTT2400.2142876275681033No Hit
GTATTGGAATTAGTGAAATTGGAGT2230.19910892061536264No Hit
CTACAAACACTTATTACAACCCAAG2080.18571594389235618No Hit
CATTTACACCTACTACCCAACTATC1980.1767872927436852No Hit
CATCTAGCCTATCAGTTTACTCCAT1940.17321583228421683No Hit
GTATGGTGGTACTCCCGCTGTAAAA1910.17053723693961553No Hit
GCTATAAAGAATAACGCGAATGGGC1780.15892999044634326No Hit
GTTAAAGGGCGTATTGGTTCTTTTA1650.14732274395307102No Hit
GGTATCAACGCAGAGTACTTTTTTT1640.1464298788382039No Hit
CATTAGTAGAACGCAAAATCTTAGG1570.14017982303413423No Hit
CACTTATTACAACCCAAGAACACAT1560.13928695791926715No Hit
ATCCTACTCTCTACAAACACTTATT1530.13660836257466585No Hit
ATGCTAGTGTTAGGGGTAGAAAGTT1490.13303690211519745No Hit
AGTGTACTCTGCTATAAAGAATAAC1470.13125117188546326No Hit
GAGTAGGATCCATTTATTAATAGAA1350.1205367905070581No Hit
GTGGTACTCCCGCTGTAAAAATTGG1340.11964392539219101No Hit
GCTAATGCCATTGGTTGAATAAATA1330.1187510602773239No Hit
GTGTTGGAAAGAATGGAGACGGTTG1260.11250100447325422No Hit
GTGTAAATGTATGTGGTAAAAGGCC1240.11071527424352003No Hit
GTACATGGGGTGTTCTTTATTAATA1230.10982240912865295No Hit
ATATAAGCCTCGTCCGACATGAAGG1230.10982240912865295No Hit
GCCATAATATGATTTATCTCAACCC1230.10982240912865295No Hit
ACCAATACCACACCCATTAATTAAT1200.10714381378405165No Hit
ATATAGAGGTTGTTAAAGGGCGTAT1200.10714381378405165No Hit
GATAAAAGGATAATAGCTATGGTTA1200.10714381378405165No Hit
TAGCTATGGTTACTTCATATGAAAT1190.10625094866918454No Hit
ATGTATAAGTTGATCGTAACGGAAG1190.10625094866918454No Hit
GTGTAAGCATCTGGGTAGTCTGAGT1170.10446521843945035No Hit
GTGTTTGTAGAGAGTAGGATCCATT1160.10357235332458326No Hit
ATCCTATTCCCATCCTCAAAACGCC1150.10267948820971615No Hit
ATCTTAGGGTACATACAACTACGAA1130.10089375797998196No Hit
GTCTATGAGTTCCCCTACCAATACC1130.10089375797998196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAGGC250.00596775219.0165639
CACAAAT250.005998711618.99955215
TAGTGAA456.67165E-414.78404211
CATAACA400.00525506914.2432952
GGTATCA604.070947E-412.655051
AGTGAAA550.003028291712.09603512
CACTATT657.893563E-411.69726412
GTATTAA752.0587053E-411.38954451
TACACCT600.00581908511.0830745
GTATCAA2350.010.9048831
TATACTA1002.3448523E-510.4497545
TCTCTAC900.00109234289.5040278
CTATACT1002.7044842E-49.4997764
ATCAACG2303.6379788E-129.495533
TATAGTA800.00447775429.495532
TCAACGC2355.456968E-129.2976544
GTATAGG850.00740083738.9329761
AACGCAG2502.3646862E-118.7437056
CAACGCA2502.3646862E-118.7397945
TATCAAC2553.6379788E-118.5645952
Produced by FastQC (version 0.11.5)