##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062253_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	111999
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29498477664979	32.0	32.0	32.0	32.0	32.0
2	30.897990160626435	32.0	32.0	32.0	32.0	32.0
3	30.81277511406352	32.0	32.0	32.0	32.0	32.0
4	30.926883275743535	32.0	32.0	32.0	32.0	32.0
5	30.871588139179813	32.0	32.0	32.0	32.0	32.0
6	34.42184305217011	36.0	36.0	36.0	32.0	36.0
7	34.19241243225386	36.0	36.0	36.0	32.0	36.0
8	33.93836552112072	36.0	36.0	36.0	32.0	36.0
9	34.45729872588148	36.0	36.0	36.0	32.0	36.0
10	33.98340163751462	36.0	36.0	36.0	32.0	36.0
11	34.19361780015893	36.0	36.0	36.0	32.0	36.0
12	34.119259993392795	36.0	36.0	36.0	32.0	36.0
13	34.19503745569157	36.0	36.0	36.0	32.0	36.0
14	34.113706372378324	36.0	36.0	36.0	32.0	36.0
15	34.286431128849365	36.0	36.0	36.0	32.0	36.0
16	34.27712747435245	36.0	36.0	36.0	32.0	36.0
17	34.02171447959357	36.0	36.0	36.0	32.0	36.0
18	34.22931454745132	36.0	36.0	36.0	32.0	36.0
19	34.15897463370209	36.0	36.0	36.0	32.0	36.0
20	34.052116536754795	36.0	36.0	36.0	32.0	36.0
21	33.99653568335432	36.0	36.0	36.0	32.0	36.0
22	34.105117009973306	36.0	36.0	36.0	32.0	36.0
23	33.935874427450244	36.0	36.0	36.0	32.0	36.0
24	33.90104375931928	36.0	36.0	36.0	32.0	36.0
25	33.46694166912204	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	2.0
5	11.0
6	34.0
7	5.0
8	19.0
9	20.0
10	6.0
11	2.0
12	4.0
13	3.0
14	21.0
15	58.0
16	93.0
17	128.0
18	137.0
19	167.0
20	222.0
21	249.0
22	307.0
23	399.0
24	534.0
25	792.0
26	1050.0
27	1340.0
28	1829.0
29	2623.0
30	3635.0
31	5358.0
32	8365.0
33	13086.0
34	29968.0
35	41532.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.88730155895641	22.882551474133468	14.381506812621653	23.848640154288468
2	14.121554656738006	23.03413423334137	39.890534736917296	22.953776373003333
3	15.851175913855606	29.198735691708784	30.00232147002625	24.947766924409365
4	10.780426823823555	19.27225645146888	41.14206625591571	28.805250468791854
5	11.50503345213531	39.21090477083724	36.93044278300327	12.35361899402417
6	26.653680852774826	42.163388940018045	19.319674401565447	11.863255805641682
7	26.154162494635962	31.85792447432413	25.601666428264913	16.386246602774996
8	24.161883816982247	35.305476586386305	22.49012140392283	18.042518192708613
9	25.561019222172554	16.39427805096111	22.160035762181494	35.88466696468485
10	14.96123545770775	27.91583578499316	34.94800096576022	22.174927791538867
11	29.70024323937616	26.8895764773215	25.765488624982112	17.644691658320216
12	19.791657352349443	28.989135780390757	30.381365404390397	20.837841462869406
13	25.1906827021952	23.464926007063976	29.15008718200921	22.194304108731615
14	20.459544032185963	22.078676799284757	28.91014751899866	28.551631649530623
15	23.087722435840114	30.84324420996155	23.459715639810426	22.60931771438791
16	22.262157015261646	29.399458198106377	27.005158740802333	21.333226045829644
17	18.77671083454035	30.85313734956994	27.790891046617674	22.579260769272036
18	19.517777161912083	30.542567742742964	28.681262683604956	21.25839241173999
19	22.008187929061783	28.709596681922196	28.621102688787186	20.661112700228834
20	20.830391061452513	28.987709497206705	28.756201117318437	21.425698324022346
21	22.06122905027933	28.766033519553076	27.818547486033516	21.354189944134077
22	21.4036687406136	27.722055352928553	29.80494171493957	21.069334191518273
23	21.3175557919009	29.509603267524064	28.15379527924997	21.019045661325066
24	21.034849527623102	29.38300515726531	27.971684200176973	21.61046111493462
25	21.002860207365035	28.53324991061852	29.448516267429387	21.01537361458706
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	37.0
1	37.0
2	44.5
3	52.0
4	52.0
5	52.0
6	98.0
7	144.0
8	144.0
9	144.0
10	162.0
11	180.0
12	180.0
13	180.0
14	280.5
15	381.0
16	381.0
17	381.0
18	1202.0
19	2023.0
20	2023.0
21	2023.0
22	2599.5
23	3176.0
24	3176.0
25	3176.0
26	5105.0
27	7034.0
28	7034.0
29	7034.0
30	9258.5
31	11483.0
32	11483.0
33	11483.0
34	12873.5
35	14264.0
36	14264.0
37	14264.0
38	14912.5
39	15561.0
40	15561.0
41	15561.0
42	14684.5
43	13808.0
44	13808.0
45	13808.0
46	13847.5
47	13887.0
48	13887.0
49	13887.0
50	12147.0
51	10407.0
52	10407.0
53	10407.0
54	9011.0
55	7615.0
56	7615.0
57	7615.0
58	6509.0
59	5403.0
60	5403.0
61	5403.0
62	4529.5
63	3656.0
64	3656.0
65	3656.0
66	2709.5
67	1763.0
68	1763.0
69	1763.0
70	1193.5
71	624.0
72	624.0
73	624.0
74	459.5
75	295.0
76	295.0
77	295.0
78	217.5
79	140.0
80	140.0
81	140.0
82	78.0
83	16.0
84	16.0
85	16.0
86	13.5
87	11.0
88	11.0
89	11.0
90	7.5
91	4.0
92	4.0
93	4.0
94	4.0
95	4.0
96	4.0
97	4.0
98	17.5
99	31.0
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.928651148670969E-4
2	0.0
3	8.928651148670969E-4
4	0.008035786033803873
5	0.04285752551362066
6	0.07321493941910195
7	0.12767971142599488
8	0.1258939811962607
9	0.13303690211519745
10	0.1517870695274065
11	0.15625139510174196
12	0.1464298788382039
13	0.1464298788382039
14	0.13303690211519745
15	0.1508942044125394
16	0.13392976723006456
17	0.13660836257466585
18	0.12678684631112777
19	0.11339386958812132
20	0.11071527424352003
21	0.11071527424352003
22	0.1205367905070581
23	0.09821516263538069
24	0.10535808355431744
25	0.10625094866918454
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	111999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.766301484834685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	56.27209092799224	14.499236600326789
2	13.705038464204033	7.062563058598738
3	7.678979832282209	5.935767283636461
4	4.747383741076998	4.892900829471692
5	3.2677247210478897	4.2098590165983625
6	2.488044909557142	3.8464629148474536
7	1.656386443967011	2.9875266743453066
8	1.3514450065839627	2.7857391583853426
9	0.9529419918220251	2.209841159296065
>10	7.1384018296486245	34.820846614701914
>50	0.5682999514865895	9.939374458700524
>100	0.17326218033127727	6.80988223109135
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	381	0.34018160876436393	No Hit
GTATAGGGGTCCTAGGAAGATAATA	326	0.29107402744667366	No Hit
TCCTAACACTCCTCGTCCCCATTCT	321	0.28660970187233814	No Hit
CCCCTATACTATATCAATTTACCAG	264	0.23571639032491362	No Hit
TATCAACGCAGAGTACTTTTTTTTT	246	0.2196448182573059	No Hit
GTATAGTAGGGGTGAAATGGAATTT	240	0.2142876275681033	No Hit
GTATTGGAATTAGTGAAATTGGAGT	223	0.19910892061536264	No Hit
CTACAAACACTTATTACAACCCAAG	208	0.18571594389235618	No Hit
CATTTACACCTACTACCCAACTATC	198	0.1767872927436852	No Hit
CATCTAGCCTATCAGTTTACTCCAT	194	0.17321583228421683	No Hit
GTATGGTGGTACTCCCGCTGTAAAA	191	0.17053723693961553	No Hit
GCTATAAAGAATAACGCGAATGGGC	178	0.15892999044634326	No Hit
GTTAAAGGGCGTATTGGTTCTTTTA	165	0.14732274395307102	No Hit
GGTATCAACGCAGAGTACTTTTTTT	164	0.1464298788382039	No Hit
CATTAGTAGAACGCAAAATCTTAGG	157	0.14017982303413423	No Hit
CACTTATTACAACCCAAGAACACAT	156	0.13928695791926715	No Hit
ATCCTACTCTCTACAAACACTTATT	153	0.13660836257466585	No Hit
ATGCTAGTGTTAGGGGTAGAAAGTT	149	0.13303690211519745	No Hit
AGTGTACTCTGCTATAAAGAATAAC	147	0.13125117188546326	No Hit
GAGTAGGATCCATTTATTAATAGAA	135	0.1205367905070581	No Hit
GTGGTACTCCCGCTGTAAAAATTGG	134	0.11964392539219101	No Hit
GCTAATGCCATTGGTTGAATAAATA	133	0.1187510602773239	No Hit
GTGTTGGAAAGAATGGAGACGGTTG	126	0.11250100447325422	No Hit
GTGTAAATGTATGTGGTAAAAGGCC	124	0.11071527424352003	No Hit
GTACATGGGGTGTTCTTTATTAATA	123	0.10982240912865295	No Hit
ATATAAGCCTCGTCCGACATGAAGG	123	0.10982240912865295	No Hit
GCCATAATATGATTTATCTCAACCC	123	0.10982240912865295	No Hit
ACCAATACCACACCCATTAATTAAT	120	0.10714381378405165	No Hit
ATATAGAGGTTGTTAAAGGGCGTAT	120	0.10714381378405165	No Hit
GATAAAAGGATAATAGCTATGGTTA	120	0.10714381378405165	No Hit
TAGCTATGGTTACTTCATATGAAAT	119	0.10625094866918454	No Hit
ATGTATAAGTTGATCGTAACGGAAG	119	0.10625094866918454	No Hit
GTGTAAGCATCTGGGTAGTCTGAGT	117	0.10446521843945035	No Hit
GTGTTTGTAGAGAGTAGGATCCATT	116	0.10357235332458326	No Hit
ATCCTATTCCCATCCTCAAAACGCC	115	0.10267948820971615	No Hit
ATCTTAGGGTACATACAACTACGAA	113	0.10089375797998196	No Hit
GTCTATGAGTTCCCCTACCAATACC	113	0.10089375797998196	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGAGGC	25	0.005967752	19.016563	9
CACAAAT	25	0.0059987116	18.999552	15
TAGTGAA	45	6.67165E-4	14.784042	11
CATAACA	40	0.005255069	14.243295	2
GGTATCA	60	4.070947E-4	12.65505	1
AGTGAAA	55	0.0030282917	12.096035	12
CACTATT	65	7.893563E-4	11.697264	12
GTATTAA	75	2.0587053E-4	11.3895445	1
TACACCT	60	0.005819085	11.083074	5
GTATCAA	235	0.0	10.904883	1
TATACTA	100	2.3448523E-5	10.449754	5
TCTCTAC	90	0.0010923428	9.504027	8
CTATACT	100	2.7044842E-4	9.499776	4
ATCAACG	230	3.6379788E-12	9.49553	3
TATAGTA	80	0.0044777542	9.49553	2
TCAACGC	235	5.456968E-12	9.297654	4
GTATAGG	85	0.0074008373	8.932976	1
AACGCAG	250	2.3646862E-11	8.743705	6
CAACGCA	250	2.3646862E-11	8.739794	5
TATCAAC	255	3.6379788E-11	8.564595	2
>>END_MODULE