FastQCFastQC Report
Thu 2 Feb 2017
SRR4062253_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062253_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences111999
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA3480.31071705997374977No Hit
TCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCTTCCTAACATTA3050.27232386003446457No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT2130.19018026946669167No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT2080.18571594389235618No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT2000.1785730229734194No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA1920.17143010205448264No Hit
CATCTAGCCTATCAGTTTACTCCATTCTATGATCAGGATGAGCCTCAAAC1920.17143010205448264No Hit
GCTATAAAGAATAACGCGAATGGGCCGGCTGCGTATTCTACGTTAAACCC1890.16875150670988134No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1810.16160858579094456No Hit
GTTAAAGGGCGTATTGGTTCTTTTATAAATAATTTTATGGCGTCTGCAAA1800.16071572067607745No Hit
CTACAAACACTTATTACAACCCAAGAACACATATGATTACTTCTGCCAGC1640.1464298788382039No Hit
CACTTATTACAACCCAAGAACACATATGATTACTTCTGCCAGCCTGACCC1470.13125117188546326No Hit
CATTAGTAGAACGCAAAATCTTAGGGTACATACAACTACGAAAAGGCCCT1450.12946544165572907No Hit
ACCAATACCACACCCATTAATTAATTTAAACCTAGGGATTTTATTTATTT1410.1258939811962607No Hit
AGTGTACTCTGCTATAAAGAATAACGCGAATGGGCCGGCTGCGTATTCTA1400.12500111608139358No Hit
ATATAGAGGTTGTTAAAGGGCGTATTGGTTCTTTTATAAATAATTTTATG1380.12321538585165939No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG1360.1214296556219252No Hit
ATCCTACTCTCTACAAACACTTATTACAACCCAAGAACACATATGATTAC1360.1214296556219252No Hit
ATGTTAGTGTACTCTGCTATAAAGAATAACGCGAATGGGCCGGCTGCGTA1320.11785819516245681No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA1310.1169653300475897No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC1300.11607246493272262No Hit
TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC1280.1142867347029884No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT1280.1142867347029884No Hit
GAGTAGGATCCATTTATTAATAGAACTGATAAAAGGATAATAGCTATGGT1210.10803667889891874No Hit
GTACATGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCT1200.10714381378405165No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT1200.10714381378405165No Hit
TACTAATTCTGATTCTCCTTCTGTCAGGTCGAAGGGGGCCCGGTTTGTTT1170.10446521843945035No Hit
GATAAAAGGATAATAGCTATGGTTACTTCATATGAAATTGTTTGGGCTAC1150.10267948820971615No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT1150.10267948820971615No Hit
CTGCTATAAAGAATAACGCGAATGGGCCGGCTGCGTATTCTACGTTAAAC1140.10178662309484907No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC1130.10089375797998196No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAGTC254.4218294E-543.991967
GGTATCA850.038.8337821
AAAAGTA353.194431E-431.43686941
GTTTAGT300.005715669629.341081
CCTTCAC554.9154623E-627.99488415
ACCTTCA406.999552E-427.49497614
GGGTCCT858.08177E-925.8776257
GTTTGTT450.001388173324.45089743
GGGGTAA450.001391794824.43997812
TATAGGG901.48447725E-824.4399782
AATAGTT901.48447725E-824.43997822
GGACAAT450.001391794824.4399786
ACTAATG551.578226E-424.00633830
CTAATGT551.578226E-424.00633831
CCTTAGG551.5830214E-423.9956158
CTTAGGC551.5830214E-423.9956159
GAAGATA852.3173743E-723.28986216
TAGGGCG952.6231646E-823.1640131
GTATAGG952.6231646E-823.164011
GTATCAA2000.023.1060981
Produced by FastQC (version 0.11.5)