##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062253_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	111999
Sequences flagged as poor quality	0
Sequence length	50
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209198296413362	32.0	32.0	32.0	32.0	32.0
2	31.464879150706704	32.0	32.0	32.0	32.0	32.0
3	31.541192332074395	32.0	32.0	32.0	32.0	32.0
4	31.62566630059197	32.0	32.0	32.0	32.0	32.0
5	31.58133554763882	32.0	32.0	32.0	32.0	32.0
6	35.17755515674247	36.0	36.0	36.0	36.0	36.0
7	35.18898383021277	36.0	36.0	36.0	36.0	36.0
8	35.16162644309324	36.0	36.0	36.0	36.0	36.0
9	35.262145197724976	36.0	36.0	36.0	36.0	36.0
10	35.12886722202877	36.0	36.0	36.0	36.0	36.0
11	35.2539665532728	36.0	36.0	36.0	36.0	36.0
12	35.17822480557862	36.0	36.0	36.0	36.0	36.0
13	35.23213600121429	36.0	36.0	36.0	36.0	36.0
14	35.18850168305074	36.0	36.0	36.0	36.0	36.0
15	35.16483182885562	36.0	36.0	36.0	36.0	36.0
16	35.200108929544015	36.0	36.0	36.0	36.0	36.0
17	35.15877820337682	36.0	36.0	36.0	36.0	36.0
18	35.171760462146985	36.0	36.0	36.0	36.0	36.0
19	35.141510192055286	36.0	36.0	36.0	36.0	36.0
20	35.16601933945839	36.0	36.0	36.0	36.0	36.0
21	35.14957276404254	36.0	36.0	36.0	36.0	36.0
22	35.14356378181948	36.0	36.0	36.0	36.0	36.0
23	35.122858239805716	36.0	36.0	36.0	36.0	36.0
24	35.09272404217895	36.0	36.0	36.0	36.0	36.0
25	35.07486673988161	36.0	36.0	36.0	36.0	36.0
26	35.044446825418085	36.0	36.0	36.0	36.0	36.0
27	35.029812766185415	36.0	36.0	36.0	36.0	36.0
28	35.02866097018723	36.0	36.0	36.0	36.0	36.0
29	35.009178653380836	36.0	36.0	36.0	36.0	36.0
30	34.99828569897946	36.0	36.0	36.0	36.0	36.0
31	35.003276814971564	36.0	36.0	36.0	36.0	36.0
32	34.92275823891285	36.0	36.0	36.0	36.0	36.0
33	34.94041911088492	36.0	36.0	36.0	36.0	36.0
34	34.92459754104947	36.0	36.0	36.0	36.0	36.0
35	34.91312422432343	36.0	36.0	36.0	36.0	36.0
36	34.90807060777328	36.0	36.0	36.0	36.0	36.0
37	34.8585790944562	36.0	36.0	36.0	32.0	36.0
38	34.86537379798034	36.0	36.0	36.0	32.0	36.0
39	34.865391655282636	36.0	36.0	36.0	32.0	36.0
40	34.83611460816614	36.0	36.0	36.0	32.0	36.0
41	34.81565906838454	36.0	36.0	36.0	32.0	36.0
42	34.798301770551525	36.0	36.0	36.0	32.0	36.0
43	34.777051580817684	36.0	36.0	36.0	32.0	36.0
44	34.71455102277699	36.0	36.0	36.0	32.0	36.0
45	34.70150626344878	36.0	36.0	36.0	32.0	36.0
46	34.66938097661586	36.0	36.0	36.0	32.0	36.0
47	34.608648291502604	36.0	36.0	36.0	32.0	36.0
48	34.590817775158705	36.0	36.0	36.0	32.0	36.0
49	34.57292475825677	36.0	36.0	36.0	32.0	36.0
50	34.09313476013179	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	1.0
21	2.0
22	3.0
23	29.0
24	63.0
25	132.0
26	251.0
27	477.0
28	858.0
29	1317.0
30	2104.0
31	3148.0
32	4866.0
33	8110.0
34	19234.0
35	71403.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.42705058588168	23.282545012860815	14.762253501000286	23.528150900257216
2	13.858778148829401	23.11195242602282	40.15661553297498	22.872653892172796
3	15.593889231153849	29.475263171992605	30.14759060348753	24.783256993366013
4	10.82063232707435	19.78321235011027	41.238761060366606	28.157394262448772
5	11.675997107117029	39.31463673782802	36.53961196082108	12.469754194233877
6	26.65315452061644	42.040929302308975	19.503026839764996	11.802889337309596
7	26.344878079268565	31.971714033161007	25.290404378610525	16.393003508959904
8	24.28325252904044	35.26281484656113	22.68413110831347	17.769801516084964
9	25.803801819659107	16.282288234716383	22.244841471798853	35.66906847382566
10	14.875132813685838	28.101143760212143	34.65388083822177	22.36984258788025
11	29.952053143331636	26.723452887972215	25.62880025714515	17.695693711550998
12	19.834105661657695	28.858293377619443	30.314556379967676	20.993044580755186
13	25.662729131510105	22.92966901490192	29.026152019214457	22.38144983437352
14	21.12340288752578	21.980553397798193	28.727935070848847	28.16810864382718
15	23.174314056375504	31.031527067205957	23.281457870159556	22.512701006258983
16	22.199503562563617	29.402310755549205	27.21030732691655	21.187878354970625
17	19.16713542085197	31.03420566255056	27.201135724426113	22.59752319217136
18	19.71535460138037	30.112768864007716	28.847578996241037	21.324297538370878
19	22.005553621014474	28.742220912686722	28.73329226153805	20.518933204760756
20	21.185903445566478	29.36008357217475	28.43775390851704	21.01625907374173
21	22.346628094893706	28.617219796605326	27.79756962115733	21.23858248734364
22	21.543942356628186	27.562746095947283	29.78151590639202	21.11179564103251
23	21.574299770533663	29.404726827918104	27.864534504772365	21.156438896775864
24	21.35057189031849	29.335607203764386	27.992714090556	21.321106815361127
25	21.37608371503317	28.037750337056583	29.3261546978098	21.260011250100447
26	21.160010357235333	28.489540085179332	29.840445003973247	20.510004553612085
27	20.420718042125376	29.828837757479974	28.143108420610897	21.60733577978375
28	21.175756277009896	26.432194006928817	30.78234936962034	21.609700346440945
29	20.381431977071223	29.44312002785739	27.858284448968295	22.317163546103092
30	20.30304112573439	28.723727209414452	28.33622028964803	22.637011375203127
31	22.21557527812997	26.931730923766494	29.28445150806264	21.568242290040892
32	20.727863641639658	29.377940874472092	27.82614130483308	22.068054179055167
33	21.024294859775534	29.872588148108463	27.842212876900685	21.260904115215315
34	21.918444244042252	27.029295427556093	30.531174942185952	20.521085386215702
35	21.46894168697935	29.677943553067436	28.174358699631245	20.678756060321966
36	21.36020286076537	27.900498223182556	28.689798032107717	22.049500883944358
37	23.67138112075431	26.207185970927533	28.829601057180614	21.29183185113754
38	22.50823668068465	27.485066830953848	28.574362271091708	21.432334217269798
39	20.42447186556903	29.13623457561742	28.036214932409507	22.403078626404042
40	21.52340645898624	28.199358922847523	29.082402521451083	21.19483209671515
41	21.413775245986535	28.063001125020087	29.051411632350575	21.4718119966428
42	22.390192069005547	27.699547285049693	27.813842183746907	22.096418462197857
43	21.192259954102635	27.551813124503298	29.412710176892375	21.843216744501692
44	20.36823730054557	28.93752288089434	28.509817577884334	22.184422240675755
45	20.901639344262296	28.041180042144365	29.06532376156291	21.99185685203043
46	21.25452029108442	27.436046252064823	28.70931738024019	22.60011607661056
47	20.755913104815306	29.03917069207211	28.521299677669937	21.683616525442645
48	20.558244191653124	29.0872725324571	27.555047591835276	22.799435684054504
49	21.391515541149893	27.916030466189852	29.039314957184825	21.653139035475434
50	20.36643511491277	28.204075072769157	28.614796692798084	22.81469311951999
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	25.0
1	16.5
2	8.0
3	26.5
4	45.0
5	43.0
6	41.0
7	36.5
8	32.0
9	41.5
10	51.0
11	73.5
12	96.0
13	144.0
14	192.0
15	267.5
16	343.0
17	296.5
18	250.0
19	206.0
20	162.0
21	202.5
22	243.0
23	525.0
24	807.0
25	1334.0
26	1861.0
27	2339.0
28	2817.0
29	3145.5
30	3474.0
31	4334.0
32	5194.0
33	5884.0
34	6574.0
35	7379.5
36	8185.0
37	8293.0
38	8401.0
39	9354.0
40	10307.0
41	10736.5
42	11166.0
43	9629.5
44	8093.0
45	8014.0
46	7935.0
47	7210.5
48	6486.0
49	5603.5
50	4721.0
51	4734.5
52	4748.0
53	4750.5
54	4753.0
55	4447.0
56	4141.0
57	3805.5
58	3470.0
59	3019.5
60	2569.0
61	2202.5
62	1836.0
63	1617.0
64	1398.0
65	1112.5
66	827.0
67	606.0
68	385.0
69	267.5
70	150.0
71	107.0
72	64.0
73	55.0
74	46.0
75	48.5
76	51.0
77	36.5
78	22.0
79	16.5
80	11.0
81	10.5
82	10.0
83	6.5
84	3.0
85	3.5
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027678818560880006
2	0.004464325574335485
3	0.0
4	0.0
5	0.0
6	8.928651148670969E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.928651148670969E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0017857302297341938
25	0.0
26	0.0
27	0.0
28	0.0026785953446012913
29	0.0
30	8.928651148670969E-4
31	8.928651148670969E-4
32	0.0
33	0.0
34	0.0017857302297341938
35	0.0
36	8.928651148670969E-4
37	0.0026785953446012913
38	0.0
39	8.928651148670969E-4
40	0.0
41	8.928651148670969E-4
42	0.007142920918936775
43	0.00892865114867097
44	0.0053571906892025825
45	0.0026785953446012913
46	0.0035714604594683876
47	0.0017857302297341938
48	0.004464325574335485
49	0.0053571906892025825
50	8.928651148670969E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	111999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.113643871820283
#Duplication Level	Percentage of deduplicated	Percentage of total
1	58.58290723155588	16.46978990883847
2	13.443643408390765	7.558996062464843
3	7.066408359005304	5.959874641737873
4	4.738463492870074	5.328619005526835
5	3.0647568837933115	4.308074179233743
6	2.1659732588052214	3.653604050036161
7	1.7721599390224538	3.487531138670881
8	1.146504906786928	2.5785944517361763
9	0.9972369549337822	2.523236814614416
>10	6.418521929685267	33.79583746283449
>50	0.47321116651316414	8.984901650907599
>100	0.13021246863785055	5.350940633398513
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA	348	0.31071705997374977	No Hit
TCCTAACACTCCTCGTCCCCATTCTAATCGCCATAGCCTTCCTAACATTA	305	0.27232386003446457	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	213	0.19018026946669167	No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT	208	0.18571594389235618	No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT	200	0.1785730229734194	No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA	192	0.17143010205448264	No Hit
CATCTAGCCTATCAGTTTACTCCATTCTATGATCAGGATGAGCCTCAAAC	192	0.17143010205448264	No Hit
GCTATAAAGAATAACGCGAATGGGCCGGCTGCGTATTCTACGTTAAACCC	189	0.16875150670988134	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181	0.16160858579094456	No Hit
GTTAAAGGGCGTATTGGTTCTTTTATAAATAATTTTATGGCGTCTGCAAA	180	0.16071572067607745	No Hit
CTACAAACACTTATTACAACCCAAGAACACATATGATTACTTCTGCCAGC	164	0.1464298788382039	No Hit
CACTTATTACAACCCAAGAACACATATGATTACTTCTGCCAGCCTGACCC	147	0.13125117188546326	No Hit
CATTAGTAGAACGCAAAATCTTAGGGTACATACAACTACGAAAAGGCCCT	145	0.12946544165572907	No Hit
ACCAATACCACACCCATTAATTAATTTAAACCTAGGGATTTTATTTATTT	141	0.1258939811962607	No Hit
AGTGTACTCTGCTATAAAGAATAACGCGAATGGGCCGGCTGCGTATTCTA	140	0.12500111608139358	No Hit
ATATAGAGGTTGTTAAAGGGCGTATTGGTTCTTTTATAAATAATTTTATG	138	0.12321538585165939	No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG	136	0.1214296556219252	No Hit
ATCCTACTCTCTACAAACACTTATTACAACCCAAGAACACATATGATTAC	136	0.1214296556219252	No Hit
ATGTTAGTGTACTCTGCTATAAAGAATAACGCGAATGGGCCGGCTGCGTA	132	0.11785819516245681	No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA	131	0.1169653300475897	No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC	130	0.11607246493272262	No Hit
TTACTATACTACTAACAGACCGCAACCTAAACACAACTTTCTTTGATCCC	128	0.1142867347029884	No Hit
GTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATACATAAT	128	0.1142867347029884	No Hit
GAGTAGGATCCATTTATTAATAGAACTGATAAAAGGATAATAGCTATGGT	121	0.10803667889891874	No Hit
GTACATGGGGTGTTCTTTATTAATATCCTAACACTCCTCGTCCCCATTCT	120	0.10714381378405165	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	120	0.10714381378405165	No Hit
TACTAATTCTGATTCTCCTTCTGTCAGGTCGAAGGGGGCCCGGTTTGTTT	117	0.10446521843945035	No Hit
GATAAAAGGATAATAGCTATGGTTACTTCATATGAAATTGTTTGGGCTAC	115	0.10267948820971615	No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT	115	0.10267948820971615	No Hit
CTGCTATAAAGAATAACGCGAATGGGCCGGCTGCGTATTCTACGTTAAAC	114	0.10178662309484907	No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC	113	0.10089375797998196	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	8.92865114867097E-4	0.0
26	0.0	0.0	0.0	8.92865114867097E-4	0.0
27	0.0	0.0	0.0	8.92865114867097E-4	0.0
28	0.0	0.0	0.0	0.001785730229734194	0.0
29	0.0	0.0	0.0	0.003571460459468388	0.0
30	0.0	0.0	0.0	0.006250055804069679	0.0
31	0.0	0.0	0.0	0.020535897641943232	0.0
32	0.0	0.0	0.0	0.046428985973089046	0.0
33	0.0	0.0	0.0	0.056250502236627115	0.0
34	0.0	0.0	0.0	0.06964347895963356	0.0
35	0.0	0.0	0.0	0.09732229752051358	0.0
36	0.0	0.0	0.0	0.119643925392191	0.0
37	0.0	0.0	0.0	0.183930213662622	0.0
38	0.0	0.0	0.0	0.25535942285198976	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACAGTC	25	4.4218294E-5	43.99196	7
GGTATCA	85	0.0	38.833782	1
AAAAGTA	35	3.194431E-4	31.436869	41
GTTTAGT	30	0.0057156696	29.34108	1
CCTTCAC	55	4.9154623E-6	27.994884	15
ACCTTCA	40	6.999552E-4	27.494976	14
GGGTCCT	85	8.08177E-9	25.877625	7
GTTTGTT	45	0.0013881733	24.450897	43
GGGGTAA	45	0.0013917948	24.439978	12
TATAGGG	90	1.48447725E-8	24.439978	2
AATAGTT	90	1.48447725E-8	24.439978	22
GGACAAT	45	0.0013917948	24.439978	6
ACTAATG	55	1.578226E-4	24.006338	30
CTAATGT	55	1.578226E-4	24.006338	31
CCTTAGG	55	1.5830214E-4	23.995615	8
CTTAGGC	55	1.5830214E-4	23.995615	9
GAAGATA	85	2.3173743E-7	23.289862	16
TAGGGCG	95	2.6231646E-8	23.16401	31
GTATAGG	95	2.6231646E-8	23.16401	1
GTATCAA	200	0.0	23.106098	1
>>END_MODULE