FastQCFastQC Report
Thu 2 Feb 2017
SRR4062252_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062252_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7006
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCCTGTACTGGTCATCATTTGTTGAATACCACTTCTAGTGGTATTCTTG570.8135883528404225No Hit
GTACATGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAGTCGCGTG350.49957179560376824No Hit
CTTCTAGTGGTATTCTTGTGGCTTCTTGCTGTTTCCTTGAGCCAACTGAA340.4852983157293748No Hit
GTGCACGCAGGTGCGAGTGGAATTCATGGATGACACCAGCCGCTCTATCA280.39965743648301455No Hit
CCAATAGGGTGAGCACGTCACCCTCTCGAACGGGGCCTTTGACATTTCGG250.35683699685983444No Hit
CACAAACACCATTCAGAAATCAGCAACAGCAGTTCAATCAAGAAGAAACT240.3425635169854411No Hit
GTCCACCAGTACCTGTTTAGAAATAGTTCTTTGAGGTGATTCCAATCTTC240.3425635169854411No Hit
ATTTAACAGTTGCAGATCATTCCGTGGGCTAAGTAGTGGATATCCAGAAC210.2997430773622609No Hit
GGGCAGGACCGGTTCGCAGGGACAGTGCACGCAGGTGCGAGTGGAATTCA200.28546959748786754No Hit
GTTTAGAAATAGTTCTTTGAGGTGATTCCAATCTTCATTGTCTGAATATC200.28546959748786754No Hit
GTACATGGGAGTCATATGGTGTTTTTTCTGAAGCAGCCATGTGTCGTTTG200.28546959748786754No Hit
CCCAGACACAGACCCTCTAGATGGTAAAGTGGGCAAAAAGCAGGTTTGGC200.28546959748786754No Hit
GTACTGGTCATCATTTGTTGAATACCACTTCTAGTGGTATTCTTGTGGCT180.2569226377390808No Hit
ATCCAGAACCCAGCTGCAGGATTAACGCAACCTTCGAGCTTCTCTTTCTG180.2569226377390808No Hit
ATATATAAGTACACTGTAGCTGTCTTCAGACACTCCAGAAGAGGGAGTCA170.2426491578646874No Hit
CTCCAATAGGGTGAGCACGTCACCCTCTCGAACGGGGCCTTTGACATTTC170.2426491578646874No Hit
ATTCAGAAATCAGCAACAGCAGTTCAATCAAGAAGAAACTGTGAGGCTCT160.22837567799029404No Hit
GGATATAGAGCATGTGTAGAGAGGTTCTGTTCAGTGGGGATATGTCCACC160.22837567799029404No Hit
GTAGTGGATATCCAGAACCCAGCTGCAGGATTAACGCAACCTTCGAGCTT160.22837567799029404No Hit
TATCAACGCAGAGTACATGGGCTCTCCGCCAGATCGCCGCCATCATGGAC160.22837567799029404No Hit
GTACAGGGTGGCCAGTTGTCCCAATAGCACAAAGTGTTGTCCACAGTCCA150.21410219811590064No Hit
CGCAGGGACAGTGCACGCAGGTGCGAGTGGAATTCATGGATGACACCAGC150.21410219811590064No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGCGCACCCGACTGC150.21410219811590064No Hit
GTTGCAGATCATTCCGTGGGCTAAGTAGTGGATATCCAGAACCCAGCTGC150.21410219811590064No Hit
TGTGTAGAGAGGTTCTGTTCAGTGGGGATATGTCCACCAGTACCTGTTTA150.21410219811590064No Hit
CCAAAGTGCTGGGCAGGACCGGTTCGCAGGGACAGTGCACGCAGGTGCGA150.21410219811590064No Hit
GACACAGACCCTCTAGATGGTAAAGTGGGCAAAAAGCAGGTTTGGCACAC150.21410219811590064No Hit
GATATAGAGCATGTGTAGAGAGGTTCTGTTCAGTGGGGATATGTCCACCA140.19982871824150727No Hit
GTGGGGATATGTCCACCAGTACCTGTTTAGAAATAGTTCTTTGAGGTGAT140.19982871824150727No Hit
ACGCAGAGTACATGGGCTCTCCGCCAGATCGCCGCCATCATGGACACGAG140.19982871824150727No Hit
GTTCTGTTCAGTGGGGATATGTCCACCAGTACCTGTTTAGAAATAGTTCT140.19982871824150727No Hit
GTTCAGGGCCAGGGATGGAACCCACATCCTCAAGGATCCTAGTTGGGTTC140.19982871824150727No Hit
GTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG140.19982871824150727No Hit
GTCTGAATATCAGCAGTCCAGTTCACACAAATCAGCTGAGGTAGCTTGAT140.19982871824150727No Hit
GGCTAGGGTAACCAAAGTGCTGGGCAGGACCGGTTCGCAGGGACAGTGCA130.1855552383671139No Hit
CCCAATGGACTGTGGACAACACTTTGTGCTATTGGGACAACTGGCCACCC130.1855552383671139No Hit
GAATATCAGCAGTCCAGTTCACACAAATCAGCTGAGGTAGCTTGATCCAC130.1855552383671139No Hit
TCTTTGAGGTGATTCCAATCTTCATTGTCTGAATATCAGCAGTCCAGTTC130.1855552383671139No Hit
CTTGAGAGAACATGGGATGTTTGGAAAGGGTTTAAATAGAACCCAATGGA130.1855552383671139No Hit
GTATCAACGCAGAGTACATGGGCTCTCCGCCAGATCGCCGCCATCATGGA130.1855552383671139No Hit
GGGTAAGCGCACCCGACTGCTCTTCCGAAGGTCCGAAGTTCAAATCCCAG120.17128175849272054No Hit
AGCTTGATCCACTCACAAACACCATTCAGAAATCAGCAACAGCAGTTCAA120.17128175849272054No Hit
TTATTTAACAGTTGCAGATCATTCCGTGGGCTAAGTAGTGGATATCCAGA120.17128175849272054No Hit
CCCTGTACTGGTCATCATTTGTTGAATACCACTTCTAGTGGTATTCTTGT120.17128175849272054No Hit
ACATAAGACACTACACCCAGACACAGACCCTCTAGATGGTAAAGTGGGCA120.17128175849272054No Hit
CTCCAGAAGAGGGAGTCAGATCTCATTTTGGATGGTTGTGAGGCACCATG120.17128175849272054No Hit
GTGTAGAGAGGTTCTGTTCAGTGGGGATATGTCCACCAGTACCTGTTTAG120.17128175849272054No Hit
TGCTAGAACAGACACTTGAGAGAACATGGGATGTTTGGAAAGGGTTTAAA110.15700827861832714No Hit
ACACTACACCCAGACACAGACCCTCTAGATGGTAAAGTGGGCAAAAAGCA110.15700827861832714No Hit
GGTTTAAATAGAACCCAATGGACTGTGGACAACACTTTGTGCTATTGGGA110.15700827861832714No Hit
GTATCAACGCAGAGTACATGGGGATCCTTAACCCACTGTTCAGGGCCAGG110.15700827861832714No Hit
TTTCTGAATGGTGTTTGTGAGTGGATCAAGCTACCTCAGCTGATTTGTGT110.15700827861832714No Hit
ATGTCCACCAGTACCTGTTTAGAAATAGTTCTTTGAGGTGATTCCAATCT110.15700827861832714No Hit
GTGTTTGTGAGTGGATCAAGCTACCTCAGCTGATTTGTGTGAACTGGACT110.15700827861832714No Hit
CTGCAATGTTAGGTCAAGTTAAAGAGGTGAGGTGCCACCTCCTCATAGTC110.15700827861832714No Hit
CTCTATATCCTGACTATGAGGAGGTGGCACCTCACCTCTTTAACTTGACC100.14273479874393377No Hit
GGGCTAAGTAGTGGATATCCAGAACCCAGCTGCAGGATTAACGCAACCTT100.14273479874393377No Hit
GTTTCAAAGTTGTTCAGACAAAAATTTATTTCAAACAGATAACTGACAGG100.14273479874393377No Hit
AGAAGAAACTGTGAGGCTCTTTCAGTTGGCTCAAGGAAACAGCAAGAAGC100.14273479874393377No Hit
AAGTAGTGGATATCCAGAACCCAGCTGCAGGATTAACGCAACCTTCGAGC100.14273479874393377No Hit
GTCCAGTTCACACAAATCAGCTGAGGTAGCTTGATCCACTCACAAACACC100.14273479874393377No Hit
GGATTAACGCAACCTTCGAGCTTCTCTTTCTGACTCCAATAGGGTGAGCA100.14273479874393377No Hit
AGTTAAAGAGGTGAGGTGCCACCTCCTCATAGTCAGGATATAGAGCATGT100.14273479874393377No Hit
AACTGAAAGAGCCTCACAGTTTCTTCTTGATTGAACTGCTGTTGCTGATT100.14273479874393377No Hit
GGTCATCATTTGTTGAATACCACTTCTAGTGGTATTCTTGTGGCTTCTTG90.1284613188695404No Hit
CCCTCTAGATGGTAAAGTGGGCAAAAAGCAGGTTTGGCACACTGCAATGT90.1284613188695404No Hit
TATCAACGCAGAGTACATGGGAGTCATATGGTGTTTTTTCTGAAGCAGCC90.1284613188695404No Hit
GTATCAACGCAGAGTACATGGGAGTCATATGGTGTTTTTTCTGAAGCAGC90.1284613188695404No Hit
CTATTGGAGTCAGAAAGAGAAGCTCGAAGGTTGCGTTAATCCTGCAGCTG90.1284613188695404No Hit
CTTTCAGTTGGCTCAAGGAAACAGCAAGAAGCCACAAGAATACCACTAGA90.1284613188695404No Hit
TTCTAGTGGTATTCTTGTGGCTTCTTGCTGTTTCCTTGAGCCAACTGAAA90.1284613188695404No Hit
TATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGC90.1284613188695404No Hit
CTTGTGGCTTCTTGCTGTTTCCTTGAGCCAACTGAAAGAGCCTCACAGTT90.1284613188695404No Hit
TCCTTGAGCCAACTGAAAGAGCCTCACAGTTTCTTCTTGATTGAACTGCT90.1284613188695404No Hit
AGTTCACACAAATCAGCTGAGGTAGCTTGATCCACTCACAAACACCATTC90.1284613188695404No Hit
GAACCCAATGGACTGTGGACAACACTTTGTGCTATTGGGACAACTGGCCA80.11418783899514702No Hit
TTGCAGATCATTCCGTGGGCTAAGTAGTGGATATCCAGAACCCAGCTGCA80.11418783899514702No Hit
ATCATGGACACGAGTCGCGTGCAGCCCATCAAGCTGGCTAGGGTAACCAA80.11418783899514702No Hit
GGTATCAACGCAGAGTACATGGGCTCTCCGCCAGATCGCCGCCATCATGG80.11418783899514702No Hit
CAAATCAGCTGAGGTAGCTTGATCCACTCACAAACACCATTCAGAAATCA80.11418783899514702No Hit
GATATATGTAATGATAGCGAATGTTCATTATACTCGAAAAGCGATTCTGA80.11418783899514702No Hit
CCTCTAGATGGTAAAGTGGGCAAAAAGCAGGTTTGGCACACTGCAATGTT80.11418783899514702No Hit
CCATCAAGCTGGCTAGGGTAACCAAAGTGCTGGGCAGGACCGGTTCGCAG80.11418783899514702No Hit
GTAGAGAGGTTCTGTTCAGTGGGGATATGTCCACCAGTACCTGTTTAGAA80.11418783899514702No Hit
GATCAAGCTACCTCAGCTGATTTGTGTGAACTGGACTGCTGATATTCAGA80.11418783899514702No Hit
ACGCAGAGTACATGGGAGTCATATGGTGTTTTTTCTGAAGCAGCCATGTG80.11418783899514702No Hit
ATGGTAAAGTGGGCAAAAAGCAGGTTTGGCACACTGCAATGTTAGGTCAA80.11418783899514702No Hit
CCTCAAAGAACTATTTCTAAACAGGTACTGGTGGACATATCCCCACTGAA80.11418783899514702No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers