FastQCFastQC Report
Thu 2 Feb 2017
SRR4062251_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062251_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86393
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT5290.6123181276260807No Hit
TATCAACGCAGAGTACTTTTTTTTT3850.4456379567789057No Hit
GGTATCAACGCAGAGTACTTTTTTT2710.3136828215248921No Hit
ACGCAGAGTACTTTTTTTTTTTTTT1810.2095077147454076No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT1040.12038012338962648No Hit
GTGCATGGCCGTTCTTAGTTGGTGG1000.11575011864387161No Hit
CTCCACTCCTGGTGGTGCCCTTCCG990.11459261745743289No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG890.10301760559304574No Hit
ACCATACTCCCCCCGGAACCCAAAG880.101860104406607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCCG250.00597535219.0063845
GCCGTTC250.00597535219.0063848
GGTATCA607.509698E-817.4023191
GGTCTCT350.002159791516.2722991
AGTTGGT400.005234249414.2465217
GTATCAA1303.7029713E-710.2223421
TATCAAC2103.9064544E-67.23632762
ATCAACG2103.9064544E-67.23632763
AACGCAG2155.501579E-67.07214266
TCAACGC2155.501579E-67.07214264
CAACGCA2155.501579E-67.07214265
AGTACTT1656.6562666E-46.911412713
ACATGGG2008.623093E-46.1734923
GAGTACT1700.0069120636.149124612
GTACATG2204.1257605E-46.04047441
TACATGG2150.0020533095.7427832
GCAGAGT2907.12591E-55.5708379
CGCAGAG2802.5996592E-45.43039568
CAGAGTA3001.1894403E-45.38514210
ACGCAGA2853.3450485E-45.3351257
Produced by FastQC (version 0.11.5)