##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062251_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86393
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.759471253458035	32.0	32.0	32.0	14.0	32.0
2	25.74389128748857	32.0	14.0	32.0	14.0	32.0
3	26.128737281955715	32.0	21.0	32.0	14.0	32.0
4	25.762075631127523	32.0	14.0	32.0	14.0	32.0
5	26.133702962045536	32.0	21.0	32.0	14.0	32.0
6	29.368860903082425	36.0	21.0	36.0	14.0	36.0
7	29.112914240737098	32.0	21.0	36.0	14.0	36.0
8	28.9146805875476	32.0	21.0	36.0	14.0	36.0
9	29.053256629588045	32.0	21.0	36.0	14.0	36.0
10	27.963573437662774	32.0	21.0	36.0	14.0	36.0
11	29.685888903036126	36.0	21.0	36.0	14.0	36.0
12	28.637678978620954	32.0	21.0	36.0	14.0	36.0
13	29.11672241964048	32.0	21.0	36.0	14.0	36.0
14	28.550785364554997	32.0	21.0	36.0	14.0	36.0
15	28.52754274073131	32.0	21.0	36.0	14.0	36.0
16	28.599064739041356	32.0	21.0	36.0	14.0	36.0
17	28.36124454527566	32.0	21.0	36.0	14.0	36.0
18	28.432998043822995	32.0	21.0	36.0	14.0	36.0
19	28.416480501892515	32.0	21.0	36.0	14.0	36.0
20	28.344900628523146	32.0	21.0	36.0	14.0	36.0
21	28.384695519312906	32.0	21.0	36.0	14.0	36.0
22	28.145567349206534	32.0	21.0	36.0	14.0	36.0
23	28.09263481995069	32.0	21.0	36.0	14.0	36.0
24	27.981491556028846	32.0	21.0	36.0	14.0	36.0
25	27.081279733311728	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	6.0
5	1.0
6	13.0
7	5.0
8	8.0
9	7.0
10	1.0
11	3.0
12	4.0
13	4.0
14	130.0
15	303.0
16	651.0
17	1090.0
18	1842.0
19	2713.0
20	3426.0
21	3824.0
22	4036.0
23	4072.0
24	4172.0
25	4165.0
26	4208.0
27	4478.0
28	4720.0
29	5012.0
30	5172.0
31	5720.0
32	6364.0
33	6558.0
34	7775.0
35	5907.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.82081674229095	19.853690156495972	12.87850726919159	27.446985832021483
2	15.685298577431043	19.81526281064438	38.21142916671489	26.28800944520968
3	18.127835910732475	22.98708213723493	29.057088619316602	29.82799333271599
4	12.455289446573058	15.644352868999526	35.07043720844089	36.829920475986526
5	14.637167731777346	35.55964619801797	33.61929239603594	16.18389367416875
6	33.91037517369153	35.48981009726725	16.141732283464567	14.458082445576656
7	29.300159844325524	30.589339078462714	20.57590288878078	19.534598188430977
8	27.612329290753028	32.62790886240169	19.12291065781701	20.636851189028274
9	25.376506024096386	14.392956441149213	19.30375347544022	40.92678405931418
10	15.836123695095527	26.319387317660965	31.08018862459303	26.764300362650477
11	36.02623376322406	21.616203751984333	22.13647582299162	20.221086661799976
12	23.890021782453537	23.706956481438567	29.128238402002133	23.274783334105763
13	28.317692040319777	19.579423010079942	25.871857258718574	26.231027690881703
14	22.515436210511684	19.443253825747483	26.266464325845952	31.774845637894884
15	23.988181449510456	27.292740860900295	23.011413011992353	25.707664677596892
16	24.733836119510187	26.64303339936746	23.90087929656275	24.7222511845596
17	22.35610343868755	26.859532857540085	26.20493094818797	24.579432755584392
18	23.2397303120873	25.32494613192465	27.479669145756585	23.955654410231457
19	23.739473897582617	25.210522048347677	27.141417534430634	23.908586519639073
20	23.87653463053046	24.54366458188557	26.770905721565903	24.80889506601807
21	24.913422671098807	23.953254033519038	26.746893061073212	24.386430234308946
22	23.85061798468649	24.422847478830985	27.345387992447495	24.38114654403503
23	22.83408030203013	24.64880890340363	27.391168398012717	25.12594239655352
24	22.90346864323354	25.200069488679137	27.460767849904457	24.435694018182872
25	23.011350474866806	24.474171878619412	27.30368311327311	25.210794533240676
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	5.0
2	32.0
3	59.0
4	59.0
5	59.0
6	102.5
7	146.0
8	146.0
9	146.0
10	193.0
11	240.0
12	240.0
13	240.0
14	319.0
15	398.0
16	398.0
17	398.0
18	669.5
19	941.0
20	941.0
21	941.0
22	1383.5
23	1826.0
24	1826.0
25	1826.0
26	2493.0
27	3160.0
28	3160.0
29	3160.0
30	3772.0
31	4384.0
32	4384.0
33	4384.0
34	5157.5
35	5931.0
36	5931.0
37	5931.0
38	6528.0
39	7125.0
40	7125.0
41	7125.0
42	8059.5
43	8994.0
44	8994.0
45	8994.0
46	9698.5
47	10403.0
48	10403.0
49	10403.0
50	10602.5
51	10802.0
52	10802.0
53	10802.0
54	10245.0
55	9688.0
56	9688.0
57	9688.0
58	8847.5
59	8007.0
60	8007.0
61	8007.0
62	6952.5
63	5898.0
64	5898.0
65	5898.0
66	4949.5
67	4001.0
68	4001.0
69	4001.0
70	3073.5
71	2146.0
72	2146.0
73	2146.0
74	1592.5
75	1039.0
76	1039.0
77	1039.0
78	851.0
79	663.0
80	663.0
81	663.0
82	468.5
83	274.0
84	274.0
85	274.0
86	205.5
87	137.0
88	137.0
89	137.0
90	90.0
91	43.0
92	43.0
93	43.0
94	26.0
95	9.0
96	9.0
97	9.0
98	41.5
99	74.0
100	74.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0011575011864387163
2	0.0
3	0.0011575011864387163
4	0.004630004745754865
5	0.019677520169458174
6	0.03819753915247763
7	0.06829256999988426
8	0.07176507355920039
9	0.08449758661002628
10	0.09723009966085215
11	0.10649010915236189
12	0.09838760084729087
13	0.09607259847441343
14	0.08102508305071013
15	0.101860104406607
16	0.085655087796465
17	0.093757596101536
18	0.08218258423714885
19	0.06945007118632296
20	0.061347562881251955
21	0.06250506406769066
22	0.07408007593207784
23	0.0509300522033035
24	0.05556005694905838
25	0.061347562881251955
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	86393.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	79.13951361800146
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.34178233461556	73.07883740580834
2	4.776879086162262	7.560797749817693
3	1.1335215222828392	2.691190258470015
4	0.4519460004972869	1.4306714664382532
5	0.2749703821795791	1.088051115252393
6	0.1711251846543125	0.8125658328799787
7	0.12432171534715011	0.6887132059310361
8	0.08483128811923184	0.5370805505075643
9	0.06289216188149946	0.4479529591517832
>10	0.5309268549531234	7.820078015579966
>50	0.03949042722791827	2.1425346960980636
>100	0.005850433663395299	1.0892086164388317
>500	0.0014626084158488248	0.6123181276260807
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	529	0.6123181276260807	No Hit
TATCAACGCAGAGTACTTTTTTTTT	385	0.4456379567789057	No Hit
GGTATCAACGCAGAGTACTTTTTTT	271	0.3136828215248921	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	181	0.2095077147454076	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	104	0.12038012338962648	No Hit
GTGCATGGCCGTTCTTAGTTGGTGG	100	0.11575011864387161	No Hit
CTCCACTCCTGGTGGTGCCCTTCCG	99	0.11459261745743289	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	89	0.10301760559304574	No Hit
ACCATACTCCCCCCGGAACCCAAAG	88	0.101860104406607	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.001157501186438716	0.0
8	0.0	0.0	0.0	0.001157501186438716	0.0
9	0.0	0.0	0.0	0.001157501186438716	0.0
10	0.0	0.0	0.0	0.001157501186438716	0.0
11	0.0	0.0	0.0	0.001157501186438716	0.0
12	0.0	0.0	0.0	0.001157501186438716	0.001157501186438716
13	0.0	0.0	0.0	0.001157501186438716	0.001157501186438716
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGCCG	25	0.005975352	19.006384	5
GCCGTTC	25	0.005975352	19.006384	8
GGTATCA	60	7.509698E-8	17.402319	1
GGTCTCT	35	0.0021597915	16.272299	1
AGTTGGT	40	0.0052342494	14.24652	17
GTATCAA	130	3.7029713E-7	10.222342	1
TATCAAC	210	3.9064544E-6	7.2363276	2
ATCAACG	210	3.9064544E-6	7.2363276	3
AACGCAG	215	5.501579E-6	7.0721426	6
TCAACGC	215	5.501579E-6	7.0721426	4
CAACGCA	215	5.501579E-6	7.0721426	5
AGTACTT	165	6.6562666E-4	6.9114127	13
ACATGGG	200	8.623093E-4	6.173492	3
GAGTACT	170	0.006912063	6.1491246	12
GTACATG	220	4.1257605E-4	6.0404744	1
TACATGG	215	0.002053309	5.742783	2
GCAGAGT	290	7.12591E-5	5.570837	9
CGCAGAG	280	2.5996592E-4	5.4303956	8
CAGAGTA	300	1.1894403E-4	5.385142	10
ACGCAGA	285	3.3450485E-4	5.335125	7
>>END_MODULE