FastQCFastQC Report
Thu 2 Feb 2017
SRR4062251_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062251_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86393
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC1310.15163265542347182No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC1140.13195513525401364No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG1130.1307976340675749No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG990.11459261745743289No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG970.11227761508455546No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT900.10417510677948444No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG890.10301760559304574No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG890.10301760559304574No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA207.7942666E-444.0249141
TAAGGCG301.2912524E-436.6661835
TTAGACT250.00234090435.199544
CCAATTT250.00234090435.1995414
AACAATA401.8008348E-532.99956532
TGACGAA401.8008348E-532.99956521
ACAATAC401.8008348E-532.99956533
GACGAAA401.8008348E-532.99956522
GTATCAA950.032.439411
GGAAGTT353.1936163E-431.42815829
CAGATAC300.005716265629.3329493
AACTGCA300.005716265629.3329497
GTTCAGG300.005716265629.3329496
GGCTCAT300.005716265629.33294920
AGTGTGG300.005716265629.33294910
TAACTGC300.005716265629.3329496
TAACAAT454.026822E-529.33294731
TACAGGA454.026822E-529.33294737
GTAGTGA454.026822E-529.33294717
GGAGGTA454.026822E-529.33294713