##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062251_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	86393
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.00730383248643	32.0	32.0	32.0	21.0	32.0
2	28.587269801951546	32.0	32.0	32.0	14.0	32.0
3	30.32269975576725	32.0	32.0	32.0	21.0	32.0
4	30.9031750257544	32.0	32.0	32.0	32.0	32.0
5	30.61815193360573	32.0	32.0	32.0	32.0	32.0
6	34.17943583392173	36.0	36.0	36.0	32.0	36.0
7	34.1971919021217	36.0	36.0	36.0	32.0	36.0
8	34.05223802854398	36.0	36.0	36.0	32.0	36.0
9	34.374104383456995	36.0	36.0	36.0	32.0	36.0
10	33.93520308358316	36.0	36.0	36.0	32.0	36.0
11	34.5875128772007	36.0	36.0	36.0	32.0	36.0
12	34.231604412394525	36.0	36.0	36.0	32.0	36.0
13	34.40967439491625	36.0	36.0	36.0	32.0	36.0
14	34.28603011818087	36.0	36.0	36.0	32.0	36.0
15	34.194714849582724	36.0	36.0	36.0	32.0	36.0
16	34.24792517912331	36.0	36.0	36.0	32.0	36.0
17	34.23390783975554	36.0	36.0	36.0	32.0	36.0
18	34.19880082877085	36.0	36.0	36.0	32.0	36.0
19	34.22906948479622	36.0	36.0	36.0	32.0	36.0
20	34.275207482087666	36.0	36.0	36.0	32.0	36.0
21	34.26347042005718	36.0	36.0	36.0	32.0	36.0
22	34.221348951882675	36.0	36.0	36.0	32.0	36.0
23	34.156922435845495	36.0	36.0	36.0	32.0	36.0
24	34.09837602583543	36.0	36.0	36.0	32.0	36.0
25	34.10044795295915	36.0	36.0	36.0	32.0	36.0
26	33.99724514717627	36.0	36.0	36.0	32.0	36.0
27	33.97789172733902	36.0	36.0	36.0	32.0	36.0
28	33.92498234810691	36.0	36.0	36.0	32.0	36.0
29	33.84847151968331	36.0	36.0	36.0	32.0	36.0
30	33.83884110981214	36.0	36.0	36.0	32.0	36.0
31	33.836144132047735	36.0	36.0	36.0	32.0	36.0
32	33.75226002106652	36.0	36.0	36.0	32.0	36.0
33	33.764193858298704	36.0	36.0	36.0	32.0	36.0
34	33.73429560265299	36.0	36.0	36.0	32.0	36.0
35	33.74554651418518	36.0	36.0	36.0	32.0	36.0
36	33.62599979164979	36.0	36.0	36.0	27.0	36.0
37	33.64544581158196	36.0	36.0	36.0	27.0	36.0
38	33.61638095679048	36.0	36.0	36.0	27.0	36.0
39	33.55896889794312	36.0	36.0	36.0	27.0	36.0
40	33.56308960216684	36.0	36.0	36.0	27.0	36.0
41	33.45641429282465	36.0	36.0	36.0	21.0	36.0
42	33.44122787725858	36.0	36.0	36.0	21.0	36.0
43	33.39205722685866	36.0	36.0	36.0	21.0	36.0
44	33.36670795087565	36.0	36.0	36.0	21.0	36.0
45	33.295174377553735	36.0	36.0	36.0	21.0	36.0
46	33.23468336555045	36.0	36.0	36.0	21.0	36.0
47	33.255830912226685	36.0	36.0	36.0	21.0	36.0
48	33.17944740893359	36.0	36.0	36.0	21.0	36.0
49	33.113562441401506	36.0	36.0	36.0	21.0	36.0
50	32.49688053430255	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	4.0
21	4.0
22	16.0
23	30.0
24	93.0
25	210.0
26	396.0
27	890.0
28	1563.0
29	2580.0
30	4187.0
31	6524.0
32	10291.0
33	16243.0
34	23661.0
35	19701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.75692008474282	18.60058579052779	13.223121360515865	25.41937276421352
2	19.164747143865824	20.4646209762365	34.81300568333083	25.55762619656685
3	22.480988043012744	22.074705126572756	28.58680680147697	26.85750002893753
4	16.287199194379173	16.459666871158543	34.95190582570347	32.301228108758814
5	20.619726135219288	32.182005486555624	30.442281203338233	16.755987174886855
6	35.68691907909206	35.46815135485514	15.005845380991515	13.83908418506129
7	30.51404627689743	29.966548215711917	18.793189263018995	20.72621624437165
8	28.64583936198535	32.316275624182516	17.323162756241825	21.714722257590314
9	25.90371905131203	13.761531605569896	17.48174041878393	42.85300892433415
10	16.35317676200618	25.608556248770153	30.150590904355678	27.887676084867984
11	38.22416167976572	20.385910895558666	20.603521118609148	20.786406306066464
12	24.048244649450766	23.104881182503213	27.789288484020698	25.057585684025323
13	29.439885175882306	18.49108145335849	24.490410102670353	27.578623268088847
14	23.279663861655457	18.647344113527716	24.410542520806082	33.662449504010745
15	25.57035870961768	26.137534290972646	21.50984454759066	26.78226245181901
16	25.925711573854365	25.786811431481716	23.027328603011817	25.260148391652105
17	24.337619946060446	26.260229416735154	23.886194483349346	25.515956153855058
18	25.137453265889597	24.93952056300858	25.711573854363202	24.211452316738622
19	25.21384834419455	25.478916115889017	24.780942900466474	24.526292639449956
20	25.720833863854708	24.299422406907965	24.548285161992293	25.43145856724503
21	26.16184181588786	24.42327503385691	24.392022501823067	25.022860648432165
22	25.61434375470235	24.59458520944984	24.705705323347956	25.085365712499858
23	24.71843783639878	24.31562742351811	25.434931070804346	25.53100366927876
24	24.367997036762663	24.9143439207334	25.18404481896472	25.533614223539214
25	24.71265033046659	24.6142627296193	25.10967323741507	25.563413702499044
26	24.708020325720835	24.708020325720835	24.940678064195016	25.64328128436332
27	25.18143831097427	24.544812658432974	24.91868554165268	25.355063488940075
28	24.603845218941352	24.949938073686525	25.32265345571979	25.12356325165233
29	24.870070491822254	25.066845693516836	25.07957820656766	24.98350560809325
30	24.826372812297436	25.08449856468192	25.58338735068062	24.505741272340032
31	25.44332808593388	25.148161866839523	24.733771645522733	24.67473840170386
32	25.350726919159182	25.094916195944066	24.321696453375313	25.23266043152144
33	24.433692544534857	24.8446054657206	25.33538596877062	25.386316020973922
34	25.076105703008345	24.691815309110694	25.343488477075688	24.888590510805273
35	25.605083745210838	24.62468024029725	25.111988239787948	24.65824777470397
36	24.012362112671166	24.989293114025443	25.057585684025323	25.940759089278064
37	25.881726528769693	24.32372993182318	25.183753313347147	24.61079022605998
38	24.885118007245957	24.20682231199287	24.99160811639832	25.916451564362852
39	25.18983239188814	25.075238448004445	24.29623113251227	25.438698027595148
40	25.47081360758395	25.880569027583256	24.216082321484382	24.43253504334842
41	24.712936382998425	25.439855542179828	25.06482081674229	24.782387258079453
42	25.305876769570908	25.73416212336933	24.614832563577224	24.345128543482538
43	25.0497731271414	24.777757199740716	24.80669506435781	25.365774608760074
44	24.915497522804092	25.234986340695464	24.494142705005327	25.355373431495114
45	24.938940398884142	25.88695581715688	24.303457536085933	24.870646247873044
46	24.584167698771893	25.466183602838193	24.896693019110344	25.05295567927957
47	24.660848226687655	25.125011575145844	25.615797759051762	24.59834243911473
48	25.632002963237337	25.97925733864247	24.092508565607925	24.29623113251227
49	24.49038650754147	25.61089953582053	24.633923300420193	25.264790656217805
50	25.32671227326921	25.280411153939646	24.639140651225244	24.7537359215659
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	3.0
2	4.0
3	4.0
4	4.0
5	5.5
6	7.0
7	7.5
8	8.0
9	8.5
10	9.0
11	18.0
12	27.0
13	52.5
14	78.0
15	108.5
16	139.0
17	176.5
18	214.0
19	240.0
20	266.0
21	327.0
22	388.0
23	467.0
24	546.0
25	698.5
26	851.0
27	1008.5
28	1166.0
29	1271.0
30	1376.0
31	1464.0
32	1552.0
33	1741.5
34	1931.0
35	2178.5
36	2426.0
37	2702.5
38	2979.0
39	3196.5
40	3414.0
41	3717.5
42	4021.0
43	4143.0
44	4265.0
45	4568.5
46	4872.0
47	5185.0
48	5498.0
49	5718.0
50	5938.0
51	5825.0
52	5712.0
53	5692.0
54	5672.0
55	5869.5
56	6067.0
57	5858.5
58	5650.0
59	5278.5
60	4907.0
61	4574.5
62	4242.0
63	3718.5
64	3195.0
65	2809.5
66	2424.0
67	2140.0
68	1856.0
69	1775.0
70	1694.0
71	1330.5
72	967.0
73	812.5
74	658.0
75	543.0
76	428.0
77	350.0
78	272.0
79	267.5
80	263.0
81	198.5
82	134.0
83	118.5
84	103.0
85	72.0
86	41.0
87	33.0
88	25.0
89	20.0
90	15.0
91	10.5
92	6.0
93	5.5
94	5.0
95	8.5
96	12.0
97	10.0
98	8.0
99	32.0
100	56.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.016205016610142026
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0011575011864387163
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0011575011864387163
31	0.0011575011864387163
32	0.0011575011864387163
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0011575011864387163
40	0.0
41	0.0011575011864387163
42	0.0023150023728774325
43	0.0011575011864387163
44	0.00578750593219358
45	0.0023150023728774325
46	0.0
47	0.0011575011864387163
48	0.0011575011864387163
49	0.004630004745754865
50	0.0023150023728774325
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	86393.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	83.70006829257
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.55976269170667	78.3095852673249
2	4.054708135691666	6.787586957276631
3	0.9486800071911603	2.382137441690878
4	0.4259379624123577	1.4260414616924981
5	0.23647854406660124	0.9896635144051023
6	0.16733277094771196	0.8403458613545078
7	0.0995699132912005	0.583380597965113
8	0.060848280344622536	0.407440417626428
9	0.06637994219413368	0.5000405125415253
>10	0.34019720374493506	5.250425381686016
>50	0.03595580202182241	2.1089671616913406
>100	0.004148746387133355	0.4143854247450604
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	131	0.15163265542347182	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	114	0.13195513525401364	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	113	0.1307976340675749	No Hit
GGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAATAGGACCGCG	99	0.11459261745743289	No Hit
GTATTGCGCCGCTAGAGGTGAAATTCTTGGACCGGCGCAAGACGGACCAG	97	0.11227761508455546	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	90	0.10417510677948444	No Hit
CCCCGATCCCCATCACGAATGGGGTTCAACGGGTTACCCGCGCCTGCCGG	89	0.10301760559304574	No Hit
TATCAACGCAGAGTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATG	89	0.10301760559304574	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.001157501186438716	0.0	0.0	0.0
6	0.0	0.001157501186438716	0.0	0.0	0.0
7	0.0	0.001157501186438716	0.0	0.0	0.0
8	0.0	0.001157501186438716	0.0	0.0	0.0
9	0.0	0.001157501186438716	0.0	0.0	0.0
10	0.0	0.001157501186438716	0.0	0.0	0.0
11	0.0	0.001157501186438716	0.0	0.0	0.0
12	0.0	0.001157501186438716	0.0	0.0	0.0
13	0.0	0.001157501186438716	0.0	0.0	0.0
14	0.0	0.001157501186438716	0.0	0.0	0.0
15	0.0	0.001157501186438716	0.0	0.0	0.0
16	0.0	0.001157501186438716	0.0	0.0	0.0
17	0.0	0.001157501186438716	0.0	0.0	0.0
18	0.0	0.001157501186438716	0.0	0.0	0.0
19	0.0	0.001157501186438716	0.0	0.0	0.0
20	0.0	0.001157501186438716	0.0	0.0	0.001157501186438716
21	0.0	0.001157501186438716	0.0	0.0	0.001157501186438716
22	0.0	0.001157501186438716	0.0	0.0	0.001157501186438716
23	0.0	0.001157501186438716	0.0	0.0	0.001157501186438716
24	0.0	0.001157501186438716	0.0	0.0	0.001157501186438716
25	0.0	0.001157501186438716	0.0	0.0	0.001157501186438716
26	0.0	0.001157501186438716	0.0	0.0	0.001157501186438716
27	0.0	0.001157501186438716	0.0	0.0	0.001157501186438716
28	0.0	0.001157501186438716	0.0	0.0	0.001157501186438716
29	0.0	0.001157501186438716	0.0	0.0	0.001157501186438716
30	0.0	0.001157501186438716	0.0	0.0	0.001157501186438716
31	0.0	0.001157501186438716	0.0	0.004630004745754864	0.001157501186438716
32	0.0	0.001157501186438716	0.0	0.009260009491509728	0.001157501186438716
33	0.0	0.001157501186438716	0.0	0.009260009491509728	0.001157501186438716
34	0.0	0.001157501186438716	0.0	0.01157501186438716	0.001157501186438716
35	0.0	0.001157501186438716	0.0	0.01157501186438716	0.001157501186438716
36	0.0	0.001157501186438716	0.0	0.01504751542370331	0.001157501186438716
37	0.0	0.001157501186438716	0.0	0.016205016610142026	0.001157501186438716
38	0.0	0.001157501186438716	0.0	0.02315002372877432	0.001157501186438716
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20	7.7942666E-4	44.024914	1
TAAGGCG	30	1.2912524E-4	36.666183	5
TTAGACT	25	0.002340904	35.19954	4
CCAATTT	25	0.002340904	35.19954	14
AACAATA	40	1.8008348E-5	32.999565	32
TGACGAA	40	1.8008348E-5	32.999565	21
ACAATAC	40	1.8008348E-5	32.999565	33
GACGAAA	40	1.8008348E-5	32.999565	22
GTATCAA	95	0.0	32.43941	1
GGAAGTT	35	3.1936163E-4	31.428158	29
CAGATAC	30	0.0057162656	29.332949	3
AACTGCA	30	0.0057162656	29.332949	7
GTTCAGG	30	0.0057162656	29.332949	6
GGCTCAT	30	0.0057162656	29.332949	20
AGTGTGG	30	0.0057162656	29.332949	10
TAACTGC	30	0.0057162656	29.332949	6
TAACAAT	45	4.026822E-5	29.332947	31
TACAGGA	45	4.026822E-5	29.332947	37
GTAGTGA	45	4.026822E-5	29.332947	17
GGAGGTA	45	4.026822E-5	29.332947	13
>>END_MODULE