FastQCFastQC Report
Thu 2 Feb 2017
SRR4062250_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062250_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69899
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAA17872.556545873331521No Hit
GTATCAACGCAGAGTACTTTTTTTT12581.7997396243150832No Hit
TATCAACGCAGAGTACTTTTTTTTT8501.2160402866993805No Hit
GGTATCAACGCAGAGTACTTTTTTT5380.7696819696991373No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5280.7553756133850269No Hit
ACGCAGAGTACTTTTTTTTTTTTTT4890.6995808237599965No Hit
GTACATGGGGTGGTATCAACGCAAA3870.5536559893560709No Hit
GAGTACTTTTTTTTTTTTTTTTTTT3740.5350577261477274No Hit
GTATCAACGCAGAGTACATGGGGTG2990.4277600537918997No Hit
TATCAACGCAGAGTACATGGGGTGG1990.28469649065079616No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1940.277543312493741No Hit
GTACATGGGTGGTATCAACGCAAAA1790.2560837780225754No Hit
GGTATCAACGCAGAGTACATGGGGT1620.23176297228858783No Hit
ATCAACGCAGAGTACTTTTTTTTTT1610.23033233665717676No Hit
ACGCAGAGTACATGGGGTGGTATCA1440.2060115309231892No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1310.1874132677148457No Hit
GTACATGGGAGTGGTATCAACGCAA1130.16166182634944706No Hit
GTATCAACGCAGAGTACATGGGTGG1020.14592483440392567No Hit
GAGTACATGGGGTGGTATCAACGCA770.11015894361864978No Hit
ATCAACGCAGAGTACATGGGGTGGT700.10014449419877253No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA6250.09.5705881
GGTGGTA1356.500818E-58.4457189
GGTATCA3200.08.3078021
GGGTGGT1409.9404286E-58.1440858
ACGCAAA1304.0313564E-48.03967419
GGGGTGG1451.495E-47.86325457
AACGCAA1356.0446945E-47.74190818
AACGCAG7450.07.52462436
TATCAAC8450.06.96647552
AGTACTT4650.06.94728413
ACTTTTT4950.06.71818516
TACTTTT4950.06.71818515
ATCAACG8600.06.62416123
GTACTTT4900.06.59283114
CAACGCA8650.06.5905895
TCAACGC8500.06.59039164
CTTTTTT5100.06.520591317
GAGTACT4850.06.464892412
AGAGTAC9000.06.4398611
GTGGTAT1800.0017757076.334288610
Produced by FastQC (version 0.11.5)