##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062250_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	69899
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.390506301949955	32.0	32.0	32.0	32.0	32.0
2	30.148528591253093	32.0	32.0	32.0	21.0	32.0
3	30.363796334711513	32.0	32.0	32.0	21.0	32.0
4	30.25548291105738	32.0	32.0	32.0	21.0	32.0
5	30.4173164136826	32.0	32.0	32.0	27.0	32.0
6	33.82328788680811	36.0	36.0	36.0	32.0	36.0
7	33.57341306742586	36.0	36.0	36.0	21.0	36.0
8	33.38177942459835	36.0	36.0	36.0	21.0	36.0
9	33.36356743301049	36.0	36.0	36.0	21.0	36.0
10	33.333109200417745	36.0	36.0	36.0	21.0	36.0
11	33.74606217542454	36.0	36.0	36.0	27.0	36.0
12	33.53670295712385	36.0	36.0	36.0	21.0	36.0
13	33.616847165195495	36.0	36.0	36.0	27.0	36.0
14	33.51047940600009	36.0	36.0	36.0	21.0	36.0
15	33.56976494656576	36.0	36.0	36.0	21.0	36.0
16	33.5153578735032	36.0	36.0	36.0	21.0	36.0
17	33.42090730911744	36.0	36.0	36.0	21.0	36.0
18	33.524084750854804	36.0	36.0	36.0	27.0	36.0
19	33.43233808781242	36.0	36.0	36.0	21.0	36.0
20	33.23346542868997	36.0	36.0	36.0	21.0	36.0
21	33.20476687792386	36.0	36.0	36.0	21.0	36.0
22	33.20901586574915	36.0	36.0	36.0	21.0	36.0
23	33.3368145467031	36.0	36.0	36.0	21.0	36.0
24	33.36242292450536	36.0	36.0	36.0	21.0	36.0
25	33.07037296670911	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	6.0
6	13.0
7	4.0
8	14.0
9	10.0
10	2.0
11	2.0
12	4.0
13	4.0
14	97.0
15	124.0
16	116.0
17	162.0
18	201.0
19	284.0
20	376.0
21	456.0
22	605.0
23	778.0
24	946.0
25	1039.0
26	1179.0
27	1353.0
28	1542.0
29	1970.0
30	2431.0
31	3342.0
32	4379.0
33	6136.0
34	13230.0
35	29093.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.04070158371364	20.682699323309347	12.98015708379233	21.29644200918468
2	15.6726133421079	22.92450535773044	38.167928010415025	23.234953289746635
3	18.900127326571194	29.297987095666606	27.634157856335566	24.16772772142663
4	12.168600575164895	18.780135349748903	33.2751491565679	35.77611491851831
5	13.003534580214938	40.59329431462057	29.53592535882429	16.867245746340206
6	29.032950674186253	37.87752998769002	17.32129054421574	15.76822879390799
7	27.061434913361023	34.986395532006306	18.970356580266362	18.98181297436632
8	28.394460595471667	32.66071863319346	17.708049894739858	21.236770876595013
9	28.50411068778825	15.802515110716433	19.16697699733593	36.52639720415938
10	17.388252148997136	28.659025787965614	27.687679083094558	26.26504297994269
11	32.88397346818905	25.943011045370547	21.59076257467444	19.58225291176597
12	25.13536742587022	28.024638303967915	26.519123334765794	20.320870935396073
13	29.290932531155995	23.264575275748463	25.984815928950006	21.45967626414554
14	23.84130621598396	22.028072185620164	26.417931824692065	27.712689773703808
15	24.791198086042147	28.36984083778634	24.957380055298483	21.88158102087303
16	22.061161641481057	29.957745470171165	24.595001074267707	23.386091814080068
17	20.101711911754172	28.529474965976647	25.770360289377553	25.598452832891628
18	20.609046896038045	27.745151402297534	28.918268542126217	22.7275331595382
19	22.92325977689631	26.394398063952572	29.537611158048488	21.14473100110263
20	24.628447066235218	24.750150339337363	29.024082930042095	21.597319664385328
21	25.831996792301524	23.75128880742353	28.684557223049605	21.732157177225343
22	23.97599679193401	25.372364802933088	28.110678276810262	22.54096012832264
23	23.04388288352781	26.33832056696972	28.97845228720739	21.639344262295083
24	22.019846213325316	27.209072554663	28.84287698497931	21.928204247032376
25	22.41362028724029	27.37087790139897	28.61520397497029	21.600297836390453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1985.0
1	1985.0
2	1120.5
3	256.0
4	256.0
5	256.0
6	483.5
7	711.0
8	711.0
9	711.0
10	649.5
11	588.0
12	588.0
13	588.0
14	430.5
15	273.0
16	273.0
17	273.0
18	457.5
19	642.0
20	642.0
21	642.0
22	1007.5
23	1373.0
24	1373.0
25	1373.0
26	1981.0
27	2589.0
28	2589.0
29	2589.0
30	3018.0
31	3447.0
32	3447.0
33	3447.0
34	3623.5
35	3800.0
36	3800.0
37	3800.0
38	4169.5
39	4539.0
40	4539.0
41	4539.0
42	5522.0
43	6505.0
44	6505.0
45	6505.0
46	8047.5
47	9590.0
48	9590.0
49	9590.0
50	10072.0
51	10554.0
52	10554.0
53	10554.0
54	9403.0
55	8252.0
56	8252.0
57	8252.0
58	7202.5
59	6153.0
60	6153.0
61	6153.0
62	5098.0
63	4043.0
64	4043.0
65	4043.0
66	3207.5
67	2372.0
68	2372.0
69	2372.0
70	1801.5
71	1231.0
72	1231.0
73	1231.0
74	910.5
75	590.0
76	590.0
77	590.0
78	434.5
79	279.0
80	279.0
81	279.0
82	176.5
83	74.0
84	74.0
85	74.0
86	50.0
87	26.0
88	26.0
89	26.0
90	17.0
91	8.0
92	8.0
93	8.0
94	7.0
95	6.0
96	6.0
97	6.0
98	9.5
99	13.0
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.008583813788466215
5	0.02575144136539865
6	0.052933518362208325
7	0.09871385856736149
8	0.1030057654615946
9	0.11588148614429392
10	0.14163292750969256
11	0.13734102061545944
12	0.1273265711955822
13	0.1273265711955822
14	0.11302021488147185
15	0.13734102061545944
16	0.120173393038527
17	0.13447974935263737
18	0.12160402866993804
19	0.09442195167312836
20	0.08154623099042904
21	0.09585258730453941
22	0.10729767235582768
23	0.07725432409619594
24	0.08869940914748423
25	0.08869940914748423
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	69899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.306971487431866
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.45248021569265	43.23953132376715
2	14.589210375215316	16.721269259932185
3	4.94545272985995	8.502267557475786
4	2.054572234565744	4.709652498605131
5	1.0135556831515091	2.904190331764403
6	0.48181341588236765	1.6566760611739797
7	0.34201263199940085	1.3719795705231834
8	0.22467983124048233	1.030057654615946
9	0.15477943929899893	0.7982946823273581
>10	0.6565643957360762	6.3734817379361655
>50	0.039943081109419076	1.3977310118885822
>100	0.032453753401403	4.19748494255998
>500	0.007489327708016077	2.7410978697835446
>1k	0.0049928851386773845	4.3562854976466046
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAA	1787	2.556545873331521	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1258	1.7997396243150832	No Hit
TATCAACGCAGAGTACTTTTTTTTT	850	1.2160402866993805	No Hit
GGTATCAACGCAGAGTACTTTTTTT	538	0.7696819696991373	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	528	0.7553756133850269	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	489	0.6995808237599965	No Hit
GTACATGGGGTGGTATCAACGCAAA	387	0.5536559893560709	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	374	0.5350577261477274	No Hit
GTATCAACGCAGAGTACATGGGGTG	299	0.4277600537918997	No Hit
TATCAACGCAGAGTACATGGGGTGG	199	0.28469649065079616	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	194	0.277543312493741	No Hit
GTACATGGGTGGTATCAACGCAAAA	179	0.2560837780225754	No Hit
GGTATCAACGCAGAGTACATGGGGT	162	0.23176297228858783	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	161	0.23033233665717676	No Hit
ACGCAGAGTACATGGGGTGGTATCA	144	0.2060115309231892	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT	131	0.1874132677148457	No Hit
GTACATGGGAGTGGTATCAACGCAA	113	0.16166182634944706	No Hit
GTATCAACGCAGAGTACATGGGTGG	102	0.14592483440392567	No Hit
GAGTACATGGGGTGGTATCAACGCA	77	0.11015894361864978	No Hit
ATCAACGCAGAGTACATGGGGTGGT	70	0.10014449419877253	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	625	0.0	9.570588	1
GGTGGTA	135	6.500818E-5	8.445718	9
GGTATCA	320	0.0	8.307802	1
GGGTGGT	140	9.9404286E-5	8.144085	8
ACGCAAA	130	4.0313564E-4	8.039674	19
GGGGTGG	145	1.495E-4	7.8632545	7
AACGCAA	135	6.0446945E-4	7.741908	18
AACGCAG	745	0.0	7.5246243	6
TATCAAC	845	0.0	6.9664755	2
AGTACTT	465	0.0	6.947284	13
ACTTTTT	495	0.0	6.718185	16
TACTTTT	495	0.0	6.718185	15
ATCAACG	860	0.0	6.6241612	3
GTACTTT	490	0.0	6.592831	14
CAACGCA	865	0.0	6.590589	5
TCAACGC	850	0.0	6.5903916	4
CTTTTTT	510	0.0	6.5205913	17
GAGTACT	485	0.0	6.4648924	12
AGAGTAC	900	0.0	6.43986	11
GTGGTAT	180	0.001775707	6.3342886	10
>>END_MODULE