FastQCFastQC Report
Thu 2 Feb 2017
SRR4062250_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062250_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69899
Sequences flagged as poor quality0
Sequence length50
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8091.1573842258115281No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA6400.915606804103063No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5300.7582368846478491No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4720.675260018026009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.5450721755676047No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2190.31330920327901685No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1790.2560837780225754No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA1650.23605487918282095No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.20887280218601123No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1460.20887280218601123No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA1420.20315025966036712No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA1300.18598263208343466No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.17453754703214638No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1000.14306356314110358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG990.14163292750969256No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA800.11445085051288288No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT780.11158957925006081No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA730.10443640109300562No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT700.10014449419877253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGACTC207.805748E-444.09
TAGGACC301.2886219E-436.6666644
ATACATG353.1871602E-431.4285721
ATCTCAT300.005708507329.33333218
TGTAGGA300.005708507329.3333322
TAGGACT300.005708507329.33333230
AGGATCG406.965571E-427.55
ACCCCCC550.00444218120.043
GTATCAA7600.018.2368411
AACGCAG8400.016.7619046
GGTATCA4450.015.8202251
TATCAAC9400.014.9787232
ACATGGG5350.014.3925233
CATGGGG2459.276846E-1114.3673474
ATCAACG9650.014.3626953
CGCAGAG10000.014.088
TACATGG5500.014.02
TCAACGC9950.013.9296494
CAGAGTA10300.013.66990410
CAACGCA10150.013.6551735
Produced by FastQC (version 0.11.5)