##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062250_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	69899
Sequences flagged as poor quality	0
Sequence length	50
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.79026881643514	32.0	32.0	32.0	32.0	32.0
2	31.23904490765247	32.0	32.0	32.0	32.0	32.0
3	31.44092190160088	32.0	32.0	32.0	32.0	32.0
4	31.640438346757463	32.0	32.0	32.0	32.0	32.0
5	31.573126940299574	32.0	32.0	32.0	32.0	32.0
6	35.162534514084605	36.0	36.0	36.0	36.0	36.0
7	35.14094622240661	36.0	36.0	36.0	36.0	36.0
8	35.122948826163466	36.0	36.0	36.0	36.0	36.0
9	35.09735475471752	36.0	36.0	36.0	36.0	36.0
10	35.049128027582654	36.0	36.0	36.0	36.0	36.0
11	35.16081775132692	36.0	36.0	36.0	36.0	36.0
12	35.071059671812186	36.0	36.0	36.0	36.0	36.0
13	35.109257643170864	36.0	36.0	36.0	36.0	36.0
14	35.05486487646461	36.0	36.0	36.0	36.0	36.0
15	35.092676576202805	36.0	36.0	36.0	36.0	36.0
16	35.06586646447016	36.0	36.0	36.0	36.0	36.0
17	35.048384097054324	36.0	36.0	36.0	36.0	36.0
18	35.06105952874862	36.0	36.0	36.0	36.0	36.0
19	35.0043634386758	36.0	36.0	36.0	36.0	36.0
20	34.884476172763556	36.0	36.0	36.0	32.0	36.0
21	34.85546288215854	36.0	36.0	36.0	32.0	36.0
22	34.91360391421909	36.0	36.0	36.0	36.0	36.0
23	34.92688021287858	36.0	36.0	36.0	36.0	36.0
24	34.9261935077755	36.0	36.0	36.0	36.0	36.0
25	34.944434112075996	36.0	36.0	36.0	36.0	36.0
26	34.92510622469563	36.0	36.0	36.0	36.0	36.0
27	34.91865405799797	36.0	36.0	36.0	32.0	36.0
28	34.89914018798552	36.0	36.0	36.0	32.0	36.0
29	34.87301678135596	36.0	36.0	36.0	32.0	36.0
30	34.83014063148257	36.0	36.0	36.0	32.0	36.0
31	34.80820898725304	36.0	36.0	36.0	32.0	36.0
32	34.758894977038295	36.0	36.0	36.0	32.0	36.0
33	34.645202363410064	36.0	36.0	36.0	32.0	36.0
34	34.56262607476502	36.0	36.0	36.0	32.0	36.0
35	34.53153836249446	36.0	36.0	36.0	32.0	36.0
36	34.535916107526575	36.0	36.0	36.0	32.0	36.0
37	34.48179516159029	36.0	36.0	36.0	32.0	36.0
38	34.4523955993648	36.0	36.0	36.0	32.0	36.0
39	34.37572783587748	36.0	36.0	36.0	32.0	36.0
40	34.32705761169688	36.0	36.0	36.0	32.0	36.0
41	34.24542554256856	36.0	36.0	36.0	32.0	36.0
42	34.190603585172894	36.0	36.0	36.0	32.0	36.0
43	34.078599121589725	36.0	36.0	36.0	32.0	36.0
44	33.925249288258776	36.0	36.0	36.0	32.0	36.0
45	33.79836621410893	36.0	36.0	36.0	32.0	36.0
46	33.665045279617736	36.0	36.0	36.0	27.0	36.0
47	33.41894733830241	36.0	36.0	36.0	21.0	36.0
48	33.26744302493598	36.0	36.0	36.0	14.0	36.0
49	33.19891557819139	36.0	36.0	36.0	14.0	36.0
50	32.82486158600266	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	5.0
20	2.0
21	12.0
22	13.0
23	34.0
24	82.0
25	174.0
26	392.0
27	601.0
28	1065.0
29	1520.0
30	2152.0
31	2840.0
32	4039.0
33	5781.0
34	10415.0
35	40772.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.598294023357	20.48316922372338	14.120105335470576	21.79843141744905
2	15.435242946259944	22.02111829680078	38.96440222056888	23.5792365363704
3	18.472367272779298	28.41671554671741	28.855920685560594	24.254996494942706
4	11.819911586717978	16.98593685174323	35.044850427044736	36.14930113449405
5	12.631081989728035	38.74304353424227	30.953232521209173	17.672641954820527
6	29.143478447474212	37.27950328330877	17.51670267099672	16.06031559822029
7	26.81583427516846	33.960428618435174	19.727034721526774	19.496702384869597
8	28.596975636275197	30.907452181004018	18.759925034692916	21.73564714802787
9	28.260776262893607	14.06028698550766	20.31502596603671	37.36391078556203
10	17.502396314682613	27.123420935921832	28.741469835047713	26.632712914347845
11	33.52694602211763	24.29076238572798	22.17628292250247	20.006008669651926
12	25.309374955292636	26.45102218915864	27.875935278044032	20.363667577504685
13	29.70285697935593	21.800025751441364	26.970342923360853	21.526774345841858
14	24.561152520064663	20.25636990514886	28.17350748937753	27.008970085408947
15	25.057583084164293	26.040429762943674	27.0805018669795	21.82148528591253
16	22.186297371922343	28.000400577976798	26.09622455256871	23.717077497532156
17	20.17768494542125	26.00609450778981	28.21356528705704	25.6026552597319
18	20.576832286584928	24.711369261362822	32.25224967453039	22.459548777521853
19	22.96599379104136	23.21492439090688	33.070573255697504	20.748508562354253
20	24.061860684702214	22.046095080044065	32.42249531466831	21.469548920585417
21	25.446715975908095	21.286427559764803	32.071989584972606	21.194866879354496
22	23.379447488519148	22.601181705031546	32.24366586074193	21.77570494570738
23	22.5825834418232	23.292178715003075	33.03623800054364	21.08899984263008
24	22.00492145698017	23.8433145440499	32.40865260808607	21.74311139088386
25	22.02606618120431	23.645545715961603	32.62564557432868	21.702742528505414
26	21.12762700467818	24.212077426000373	32.918925878767936	21.741369690553512
27	21.479563370005295	24.3837537017697	32.57843459849211	21.5582483297329
28	20.87070975864486	24.393035466472092	32.92272915861911	21.81352561626393
29	20.807164623242105	24.820097569350065	32.408188958354195	21.964548849053635
30	20.729369348746708	24.718152683987636	32.48683758727252	22.06564037999313
31	21.00602297600824	24.45671611897166	32.5126253594472	22.0246355455729
32	20.815748437030575	25.02181719337902	32.302321921629776	21.860112447960628
33	21.19629751498591	24.92739524170589	32.11920056080917	21.757106682499032
34	21.399447774646273	24.778609136039144	31.98901271835076	21.83293037096382
35	21.505314811370692	25.017525286484787	31.81876707821285	21.658392823931674
36	21.106167470207012	25.709952932087727	31.293723801485	21.890155796220263
37	21.894447703114494	25.938854633113493	30.747220990285985	21.41947667348603
38	21.183421794303207	26.05759739052061	31.715761312751255	21.04321950242493
39	21.04465013805634	26.24501065823545	30.83592039943347	21.87441880427474
40	21.04894204495057	26.310819897280364	31.52834804503641	21.111890012732655
41	21.217757049457074	26.485357444312505	30.838781670696292	21.45810383553413
42	21.01889869669094	27.018984534828828	30.325183479019728	21.636933289460508
43	21.05496580731924	27.05370682995222	29.764800137342984	22.126527225385562
44	21.083578929296976	27.32410083264185	29.945062805802742	21.647257432258435
45	21.482731980886435	27.123808978797676	29.719019142178603	21.674439898137283
46	22.103636727803373	27.321239520444074	28.28120976279722	22.293913988955335
47	22.027496816835722	27.627004678178512	28.056195367601827	22.28930313738394
48	22.17262543456801	28.202927164255975	27.24151251126658	22.38293488990944
49	22.421563157217047	28.285906405138988	27.48186617451393	21.810664263130032
50	21.978855205367744	28.49253923518219	27.51112319203422	22.01748236741584
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1201.0
1	628.5
2	56.0
3	183.5
4	311.0
5	282.5
6	254.0
7	169.5
8	85.0
9	139.5
10	194.0
11	310.0
12	426.0
13	824.5
14	1223.0
15	1512.5
16	1802.0
17	1724.0
18	1646.0
19	1489.0
20	1332.0
21	1300.0
22	1268.0
23	1084.5
24	901.0
25	729.0
26	557.0
27	617.0
28	677.0
29	783.5
30	890.0
31	1039.5
32	1189.0
33	1365.5
34	1542.0
35	1529.0
36	1516.0
37	1693.5
38	1871.0
39	1932.0
40	1993.0
41	2309.0
42	2625.0
43	2788.0
44	2951.0
45	3347.5
46	3744.0
47	3999.5
48	4255.0
49	4715.5
50	5176.0
51	5237.0
52	5298.0
53	5200.0
54	5102.0
55	4975.0
56	4848.0
57	4603.0
58	4358.0
59	3785.0
60	3212.0
61	2861.0
62	2510.0
63	2032.0
64	1554.0
65	1369.0
66	1184.0
67	952.5
68	721.0
69	660.5
70	600.0
71	466.5
72	333.0
73	293.5
74	254.0
75	179.0
76	104.0
77	82.0
78	60.0
79	48.5
80	37.0
81	29.5
82	22.0
83	13.5
84	5.0
85	5.5
86	6.0
87	3.0
88	0.0
89	0.5
90	1.0
91	1.0
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03862716204809797
2	0.010014449419877251
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0014306356314110358
25	0.0
26	0.0
27	0.0
28	0.0028612712628220717
29	0.0
30	0.0042919068942331075
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0014306356314110358
44	0.0014306356314110358
45	0.0014306356314110358
46	0.0014306356314110358
47	0.0
48	0.0028612712628220717
49	0.0028612712628220717
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	69899.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.21689866807822
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.47090441691984	46.200947080788
2	14.81654592194438	18.140459806291936
3	4.984809534938069	9.15463740539922
4	1.9373685440523487	4.743987753758995
5	0.9675157747137182	2.9614157570208444
6	0.5118018228558074	1.8798552196741012
7	0.33419023136246784	1.4320662670424469
8	0.18695956999298902	0.915606804103063
9	0.1308716989950923	0.7210403582311622
>10	0.5912596401028277	6.462181147083649
>50	0.0373919139985978	1.5493783888181518
>100	0.023369946249123627	3.0071960972259975
>500	0.007010983874737088	2.83122791456244
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	809	1.1573842258115281	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	640	0.915606804103063	No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	530	0.7582368846478491	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	472	0.675260018026009	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	381	0.5450721755676047	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	219	0.31330920327901685	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	179	0.2560837780225754	No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA	165	0.23605487918282095	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146	0.20887280218601123	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	146	0.20887280218601123	No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA	142	0.20315025966036712	No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA	130	0.18598263208343466	No Hit
ATACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	122	0.17453754703214638	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	100	0.14306356314110358	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG	99	0.14163292750969256	No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA	80	0.11445085051288288	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	78	0.11158957925006081	No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	73	0.10443640109300562	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	70	0.10014449419877253	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0028612712628220717	0.0
24	0.0	0.0	0.0	0.008583813788466215	0.0
25	0.0	0.0	0.0	0.008583813788466215	0.0
26	0.0	0.0	0.0	0.010014449419877251	0.0
27	0.0	0.0	0.0	0.01716762757693243	0.0
28	0.0	0.0	0.0	0.025751441365398645	0.0
29	0.0	0.0	0.0	0.03862716204809797	0.0
30	0.0	0.0	0.0	0.07010114593914075	0.0
31	0.0	0.0	0.0	0.10300576546159458	0.0
32	0.0	0.0	0.0	0.14163292750969256	0.0
33	0.0	0.0	0.0	0.17310691140073534	0.0
34	0.0	0.0	0.0	0.20028898839754503	0.0
35	0.0	0.0	0.0	0.2432080573398761	0.0
36	0.0	0.0	0.0	0.2875577619136182	0.0
37	0.0	0.0	0.0	0.3447831871700597	0.0
38	0.0	0.0	0.0	0.42776005379189974	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGACTC	20	7.805748E-4	44.0	9
TAGGACC	30	1.2886219E-4	36.666664	4
ATACATG	35	3.1871602E-4	31.428572	1
ATCTCAT	30	0.0057085073	29.333332	18
TGTAGGA	30	0.0057085073	29.333332	2
TAGGACT	30	0.0057085073	29.333332	30
AGGATCG	40	6.965571E-4	27.5	5
ACCCCCC	55	0.004442181	20.0	43
GTATCAA	760	0.0	18.236841	1
AACGCAG	840	0.0	16.761904	6
GGTATCA	445	0.0	15.820225	1
TATCAAC	940	0.0	14.978723	2
ACATGGG	535	0.0	14.392523	3
CATGGGG	245	9.276846E-11	14.367347	4
ATCAACG	965	0.0	14.362695	3
CGCAGAG	1000	0.0	14.08	8
TACATGG	550	0.0	14.0	2
TCAACGC	995	0.0	13.929649	4
CAGAGTA	1030	0.0	13.669904	10
CAACGCA	1015	0.0	13.655173	5
>>END_MODULE