FastQCFastQC Report
Thu 2 Feb 2017
SRR4062249_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062249_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18537
Sequences flagged as poor quality0
Sequence length25
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTGCACCTTGTCGTCATGAACC710.38301774828720936No Hit
GCCGTACACTGTGGAGTTGGTCCGA610.3290715865566165No Hit
GTGCAGGATCGTGCTGGCAAAGAGG530.2859146571721422No Hit
GTTCTGATAAGAGTGTTAAAACGTT510.27512542482602365No Hit
CCCTTAGGGCACAGCCACCCAGGGA490.2643361924799051No Hit
CCTTTATATACTGCTGACCGGGGCG450.24275772778766788No Hit
CCCTAAGGGTGGTCGTGCAGGCAAC330.17802233371095647No Hit
GTCTTGAGGACCTCTGTGAACTTGC310.16723310136483788No Hit
CTCTTATGTAGACCAGGCTAGCCTC310.16723310136483788No Hit
CTCTTTGACGTCCCCTGGATCAGCA300.16183848519177862No Hit
CGCTTAAACAGGTGAAGCATGATCA290.15644386901871932No Hit
GTCTTTATTGGGTTCACACCAGGAG290.15644386901871932No Hit
GCCTTAGCCTGCACAGAAGAGCAGC280.15104925284566004No Hit
CTGTGGAGTTGGTCCGATAGAGCGA280.15104925284566004No Hit
GTGTCTCATAGCCCTGGCTGTCCTG270.14565463667260073No Hit
GTGTTTAGCTATCAAGTGGCATCCA270.14565463667260073No Hit
ATATACTGCTGACCGGGGCGCTGCA260.14026002049954148No Hit
CTACTGGAGGGAATGGCCGCCTCCT250.13486540432648217No Hit
CTTCAGGGTTATAAATGCTGCTGTT240.1294707881534229No Hit
GTACAATAAGTGATTCAGTAGAGGT240.1294707881534229No Hit
CCTCACAACCAGTGAGACTCGTAGT240.1294707881534229No Hit
GTGTTGGATGGGATCCCTCCACCCT230.1240761719803636No Hit
TCTCTGGACGTGCCACCTCACAACC230.1240761719803636No Hit
GACCAAGAGAGGCCAGGCTGCCCTG230.1240761719803636No Hit
GTATCAACGCAGAGTACATGGGGTG230.1240761719803636No Hit
GTACATGGGGTGTTCGTCATGTCGG230.1240761719803636No Hit
GGTTTAAACCGCCACTCGTCGGCCT220.11868155580730431No Hit
GGTGAAGCATGATCAGCAAGTCGCT220.11868155580730431No Hit
CACCAGGAGTCCGTTGGTCTTGAGG220.11868155580730431No Hit
GTTTAAGCGTGGATGCCACTTGATA210.11328693963424503No Hit
GTATTTGCATTGAGAGCCCTAGGCT210.11328693963424503No Hit
ACCCTGAAGTGCTCGACATCACAGA210.11328693963424503No Hit
GTACATGGGGTGCGAGGCATGCTGC210.11328693963424503No Hit
GGCATAGGGTCACTTGACGAAGGTT210.11328693963424503No Hit
GTACATGGGGGCTTGATAACAGCAG200.10789232346118574No Hit
TCTCTTGTGTGGGCAGCTTCATCCT200.10789232346118574No Hit
GAGCTGGAGAGATGTTAGAAGATGG200.10789232346118574No Hit
GGGCTACAGTGAGGGGAGGCAGATT190.10249770728812645No Hit
GCTAAACACCGACTCACAGAGGAAT190.10249770728812645No Hit
GTCAAAGAGTGAGCGCTCCGCCAAC190.10249770728812645No Hit
GTACATGGGGGAGTGACATCGTCTT190.10249770728812645No Hit
GTTCAGCAGTGTGGCCTCGCTGGCT190.10249770728812645No Hit
GTACCAGGCAGTGACAGCCACTCTG190.10249770728812645No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)