##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062249_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18537
Sequences flagged as poor quality	0
Sequence length	25
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40055025084965	32.0	32.0	32.0	32.0	32.0
2	31.152290014565462	32.0	32.0	32.0	32.0	32.0
3	31.065976155796516	32.0	32.0	32.0	32.0	32.0
4	31.15736095376814	32.0	32.0	32.0	32.0	32.0
5	31.1102120084156	32.0	32.0	32.0	32.0	32.0
6	34.72519825214436	36.0	36.0	36.0	32.0	36.0
7	34.5623347898797	36.0	36.0	36.0	32.0	36.0
8	34.4048659437881	36.0	36.0	36.0	32.0	36.0
9	34.77342612073151	36.0	36.0	36.0	32.0	36.0
10	34.367157576738414	36.0	36.0	36.0	32.0	36.0
11	34.48130765496035	36.0	36.0	36.0	32.0	36.0
12	34.48432864001726	36.0	36.0	36.0	32.0	36.0
13	34.53962345579112	36.0	36.0	36.0	32.0	36.0
14	34.49026271780763	36.0	36.0	36.0	32.0	36.0
15	34.633921346496194	36.0	36.0	36.0	32.0	36.0
16	34.63451475427523	36.0	36.0	36.0	32.0	36.0
17	34.45449641258025	36.0	36.0	36.0	32.0	36.0
18	34.60301019582457	36.0	36.0	36.0	32.0	36.0
19	34.554890219560875	36.0	36.0	36.0	32.0	36.0
20	34.404542266817714	36.0	36.0	36.0	32.0	36.0
21	34.34681987376598	36.0	36.0	36.0	32.0	36.0
22	34.51130172088256	36.0	36.0	36.0	32.0	36.0
23	34.355667044289795	36.0	36.0	36.0	32.0	36.0
24	34.326158493823165	36.0	36.0	36.0	32.0	36.0
25	34.12618007228786	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	3.0
7	1.0
8	4.0
9	4.0
10	1.0
11	0.0
12	1.0
13	0.0
14	4.0
15	6.0
16	10.0
17	7.0
18	18.0
19	24.0
20	13.0
21	26.0
22	39.0
23	53.0
24	74.0
25	96.0
26	127.0
27	191.0
28	291.0
29	368.0
30	495.0
31	688.0
32	1069.0
33	1634.0
34	4203.0
35	9086.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.61687435938933	16.156875438312564	11.026595457733182	27.19965474456492
2	17.742892593191996	19.113125101149052	35.636834439229645	27.507147866429303
3	21.098343852834873	20.13270755785726	27.884770998543452	30.884177590764416
4	15.736944324557617	14.71191195511437	33.0114372032801	36.539706517047904
5	17.047110247693055	32.99875883654417	33.37650423614484	16.57762667961794
6	35.03184713375796	33.396307891611784	16.468746626362947	15.103098348267299
7	32.27496085101787	28.98644635239484	18.494519142502295	20.244073654084993
8	28.911693221712127	32.17391304347826	17.99081825546854	20.923575479341075
9	27.978826833747434	14.329696445932807	18.61294155774009	39.07853516257967
10	18.706435402820553	24.89328362241314	29.767115145620576	26.633165829145728
11	39.259859535386276	20.232306861156133	21.210156672069154	19.297676931388438
12	24.819055849627308	22.350653559468512	26.43945122609917	26.390839364805014
13	31.246624176299015	18.86140218213244	23.571351409743976	26.320622231824565
14	24.545012691040665	18.420910514662204	24.350596748933413	32.68348004536372
15	27.2481771536592	25.617067242776127	20.399675938428302	26.735079665136375
16	28.079935187685663	24.22360248447205	22.111801242236027	25.584661085606264
17	25.557655954631382	24.283013772616798	24.542263029975693	25.617067242776127
18	25.664290343486712	23.071937783538562	23.552603154028947	27.71116871894578
19	26.44311247907554	24.531562179383336	23.311193908958366	25.714131432582754
20	26.85060201932941	24.09157172938826	23.49765131472383	25.5601749365585
21	27.334089313677843	24.28316863761542	23.267995032129164	25.11474701657757
22	27.4042874885253	22.053026621307843	23.203196716885362	27.339489173281496
23	27.35125796350286	24.068675089083253	21.725515603066622	26.85455134434726
24	26.86180266781876	22.47664308473295	23.821353350974782	26.840200896473508
25	27.18928841377821	24.009286254184214	23.048266925817945	25.75315840621963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.0
2	3.5
3	4.0
4	4.0
5	4.0
6	6.5
7	9.0
8	9.0
9	9.0
10	18.0
11	27.0
12	27.0
13	27.0
14	31.5
15	36.0
16	36.0
17	36.0
18	77.0
19	118.0
20	118.0
21	118.0
22	167.0
23	216.0
24	216.0
25	216.0
26	282.5
27	349.0
28	349.0
29	349.0
30	492.5
31	636.0
32	636.0
33	636.0
34	716.0
35	796.0
36	796.0
37	796.0
38	906.5
39	1017.0
40	1017.0
41	1017.0
42	1300.0
43	1583.0
44	1583.0
45	1583.0
46	1862.0
47	2141.0
48	2141.0
49	2141.0
50	2308.0
51	2475.0
52	2475.0
53	2475.0
54	2403.0
55	2331.0
56	2331.0
57	2331.0
58	2429.0
59	2527.0
60	2527.0
61	2527.0
62	2108.5
63	1690.0
64	1690.0
65	1690.0
66	1427.5
67	1165.0
68	1165.0
69	1165.0
70	948.0
71	731.0
72	731.0
73	731.0
74	549.0
75	367.0
76	367.0
77	367.0
78	267.5
79	168.0
80	168.0
81	168.0
82	132.5
83	97.0
84	97.0
85	97.0
86	63.5
87	30.0
88	30.0
89	30.0
90	17.5
91	5.0
92	5.0
93	5.0
94	4.5
95	4.0
96	4.0
97	4.0
98	8.0
99	12.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.005394616173059287
5	0.03236769703835572
6	0.05934077790365216
7	0.09710309111506717
8	0.11868155580730431
9	0.1240761719803636
10	0.16183848519177862
11	0.14565463667260073
12	0.1240761719803636
13	0.1240761719803636
14	0.10789232346118574
15	0.11868155580730431
16	0.11868155580730431
17	0.11868155580730431
18	0.11328693963424503
19	0.09710309111506717
20	0.08631385876894859
21	0.09710309111506717
22	0.09710309111506717
23	0.08091924259588931
24	0.10789232346118574
25	0.08091924259588931
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	18537.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.51621082160004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.83726855783931	53.25025624426822
2	7.159843723458467	9.095322867777957
3	2.5649736708000677	4.8875222527917135
4	1.690164769831833	4.294114473755192
5	1.0956344487854595	3.4795274316232403
6	0.8323424494649228	3.172034309758861
7	0.6369967725496858	2.8321734908561256
8	0.4841175471377612	2.459944974915035
9	0.32274503142517413	1.844958731186276
>10	1.3419398675046714	13.411015806225388
>50	0.033973161202649906	1.2731294168419918
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCCTGCACCTTGTCGTCATGAACC	71	0.38301774828720936	No Hit
GCCGTACACTGTGGAGTTGGTCCGA	61	0.3290715865566165	No Hit
GTGCAGGATCGTGCTGGCAAAGAGG	53	0.2859146571721422	No Hit
GTTCTGATAAGAGTGTTAAAACGTT	51	0.27512542482602365	No Hit
CCCTTAGGGCACAGCCACCCAGGGA	49	0.2643361924799051	No Hit
CCTTTATATACTGCTGACCGGGGCG	45	0.24275772778766788	No Hit
CCCTAAGGGTGGTCGTGCAGGCAAC	33	0.17802233371095647	No Hit
GTCTTGAGGACCTCTGTGAACTTGC	31	0.16723310136483788	No Hit
CTCTTATGTAGACCAGGCTAGCCTC	31	0.16723310136483788	No Hit
CTCTTTGACGTCCCCTGGATCAGCA	30	0.16183848519177862	No Hit
CGCTTAAACAGGTGAAGCATGATCA	29	0.15644386901871932	No Hit
GTCTTTATTGGGTTCACACCAGGAG	29	0.15644386901871932	No Hit
GCCTTAGCCTGCACAGAAGAGCAGC	28	0.15104925284566004	No Hit
CTGTGGAGTTGGTCCGATAGAGCGA	28	0.15104925284566004	No Hit
GTGTCTCATAGCCCTGGCTGTCCTG	27	0.14565463667260073	No Hit
GTGTTTAGCTATCAAGTGGCATCCA	27	0.14565463667260073	No Hit
ATATACTGCTGACCGGGGCGCTGCA	26	0.14026002049954148	No Hit
CTACTGGAGGGAATGGCCGCCTCCT	25	0.13486540432648217	No Hit
CTTCAGGGTTATAAATGCTGCTGTT	24	0.1294707881534229	No Hit
GTACAATAAGTGATTCAGTAGAGGT	24	0.1294707881534229	No Hit
CCTCACAACCAGTGAGACTCGTAGT	24	0.1294707881534229	No Hit
GTGTTGGATGGGATCCCTCCACCCT	23	0.1240761719803636	No Hit
TCTCTGGACGTGCCACCTCACAACC	23	0.1240761719803636	No Hit
GACCAAGAGAGGCCAGGCTGCCCTG	23	0.1240761719803636	No Hit
GTATCAACGCAGAGTACATGGGGTG	23	0.1240761719803636	No Hit
GTACATGGGGTGTTCGTCATGTCGG	23	0.1240761719803636	No Hit
GGTTTAAACCGCCACTCGTCGGCCT	22	0.11868155580730431	No Hit
GGTGAAGCATGATCAGCAAGTCGCT	22	0.11868155580730431	No Hit
CACCAGGAGTCCGTTGGTCTTGAGG	22	0.11868155580730431	No Hit
GTTTAAGCGTGGATGCCACTTGATA	21	0.11328693963424503	No Hit
GTATTTGCATTGAGAGCCCTAGGCT	21	0.11328693963424503	No Hit
ACCCTGAAGTGCTCGACATCACAGA	21	0.11328693963424503	No Hit
GTACATGGGGTGCGAGGCATGCTGC	21	0.11328693963424503	No Hit
GGCATAGGGTCACTTGACGAAGGTT	21	0.11328693963424503	No Hit
GTACATGGGGGCTTGATAACAGCAG	20	0.10789232346118574	No Hit
TCTCTTGTGTGGGCAGCTTCATCCT	20	0.10789232346118574	No Hit
GAGCTGGAGAGATGTTAGAAGATGG	20	0.10789232346118574	No Hit
GGGCTACAGTGAGGGGAGGCAGATT	19	0.10249770728812645	No Hit
GCTAAACACCGACTCACAGAGGAAT	19	0.10249770728812645	No Hit
GTCAAAGAGTGAGCGCTCCGCCAAC	19	0.10249770728812645	No Hit
GTACATGGGGGAGTGACATCGTCTT	19	0.10249770728812645	No Hit
GTTCAGCAGTGTGGCCTCGCTGGCT	19	0.10249770728812645	No Hit
GTACCAGGCAGTGACAGCCACTCTG	19	0.10249770728812645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE