FastQCFastQC Report
Thu 2 Feb 2017
SRR4062249_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062249_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18537
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCTGCACCTTGTCGTCATGAACCTCGTTGGCTGAACTCCGTCTCCTTA840.45314775853698014No Hit
GTGCAGGATCGTGCTGGCAAAGAGGAAGTCAGCAAAGGCTCGCTCCGGAG650.3506500512488536No Hit
CCTTTATATACTGCTGACCGGGGCGCTGCAGTTCGGCTACCGTCTCCTCG530.2859146571721422No Hit
GTTCTGATAAGAGTGTTAAAACGTTTTCCAAAAGTCCAGAGTTGTTGGCC510.27512542482602365No Hit
GCCGTACACTGTGGAGTTGGTCCGATAGAGCGAAGGGAGTTCTCTGGACG410.22117926309543076No Hit
CCCTTAGGGCACAGCCACCCAGGGAGTCACAGCTGTCCTGTGAGCTGCAT400.21578464692237148No Hit
CACCAGGAGTCCGTTGGTCTTGAGGACCTCTGTGAACTTGCAGATTTTCT340.18341694988401577No Hit
CTCTTTGACGTCCCCTGGATCAGCATTCTTGAGCTGGCAGCTTGTTGCAC310.16723310136483788No Hit
GTACATGGGGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCG300.16183848519177862No Hit
GCCTTAGCCTGCACAGAAGAGCAGCCTTCTGGGGCAACACTGGCTTCAGA280.15104925284566004No Hit
GTCTTTATTGGGTTCACACCAGGAGTCCGTTGGTCTTGAGGACCTCTGTG280.15104925284566004No Hit
CTTCAGGGTTATAAATGCTGCTGTTGTCAAACACCTGCTGGATGATCAGC280.15104925284566004No Hit
CCCTAAGGGTGGTCGTGCAGGCAACATCAGCCCGAGGAGGGTGTGAACCC280.15104925284566004No Hit
TCTCTGGACGTGCCACCTCACAACCAGTGAGACTCGTAGTCAAGCACGAA260.14026002049954148No Hit
GGTTTAAACCGCCACTCGTCGGCCTCCAGCTCTTCCACCAGACCAGCCAG250.13486540432648217No Hit
GTACATGGGGCAGCACCGCCATCAAACTCCCACAGCTCAGGCGGGCGCGC250.13486540432648217No Hit
GTATTTGCATTGAGAGCCCTAGGCTTCTTTCTCATCTGGGAAATTCCATG250.13486540432648217No Hit
GTTTAAGCGTGGATGCCACTTGATAGCTAAACACCGACTCACAGAGGAAT240.1294707881534229No Hit
AATCTGAAGAAGCCATGTGCAACAAGCTGCCAGCTCAAGAATGCTGATCC240.1294707881534229No Hit
CGCTTAAACAGGTGAAGCATGATCAGCAAGTCGCTCGGATGGAAAAACTG240.1294707881534229No Hit
GCCCTGGAGCGCCTCAAGGTGTTGGATGGGATCCCTCCACCCTATGACAA240.1294707881534229No Hit
ATCCAAGACCCTCCTCTTGTGTTTGCCACTGTTCTGATAAGAGTGTTAAA220.11868155580730431No Hit
ATATACTGCTGACCGGGGCGCTGCAGTTCGGCTACCGTCTCCTCGTGGGC210.11328693963424503No Hit
GTGTGAACCCCAGCAGCTGCTCGATGGGTTTAAACCGCCACTCGTCGGCC210.11328693963424503No Hit
TGCTTCACCTGTTTAAGCGTGGATGCCACTTGATAGCTAAACACCGACTC200.10789232346118574No Hit
GTACATGGGGGAGTGACATCGTCTTTAAACCCCGCGTGGCAATCCCTGAC200.10789232346118574No Hit
GTCTTGAGGACCTCTGTGAACTTGCAGATTTTCTTCTCCACATTCTTTTC200.10789232346118574No Hit
GGCATAGGGTCACTTGACGAAGGTTCAGGAGCACAGGGAGAGCTTTGTGG200.10789232346118574No Hit
CCCTATGACAAGAAAAAGCGGATGGTGGTCCCTGCTGCTCTCAAGGTTGT200.10789232346118574No Hit
CCTCAAGATCATCCAACTTTTGGATGATTATCCAAAATGCTTCATTGTGG200.10789232346118574No Hit
TACCTGGGGCGTCTGGCGCATGAGGTCGGGTGGAAGTACCAGGCAGTGAC200.10789232346118574No Hit
GTTCAGCAGTGTGGCCTCGCTGGCTCCCACCTTGTCTCCAGTCTTTATCA200.10789232346118574No Hit
CTGCTGAACATGCTGAACATCTCCCCCTTCTCCTTCGGGCTGATCATCCA190.10249770728812645No Hit
ACCCTGAAGTGCTCGACATCACAGAGCAGGCCCTGCACTCTCGCTTTCTG190.10249770728812645No Hit
GTCAAAGAGTGAGCGCTCCGCCAACCTCCAACTCCACGGTAAGGAGACGG190.10249770728812645No Hit
GGTGAAGCATGATCAGCAAGTCGCTCGGATGGAAAAACTGGCTGGTCTGG190.10249770728812645No Hit
CCCATATCCTCATCTGATTCCTCCGACTCTTCCTTTGCTTCAGCTTTGGC190.10249770728812645No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCTGCC450.001346921724.4444432
Produced by FastQC (version 0.11.5)