##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062249_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18537
Sequences flagged as poor quality	0
Sequence length	50
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.23471974968981	32.0	32.0	32.0	32.0	32.0
2	31.485299670928413	32.0	32.0	32.0	32.0	32.0
3	31.55213896531262	32.0	32.0	32.0	32.0	32.0
4	31.650213087338837	32.0	32.0	32.0	32.0	32.0
5	31.605383826940713	32.0	32.0	32.0	32.0	32.0
6	35.22630414845984	36.0	36.0	36.0	36.0	36.0
7	35.24772077466688	36.0	36.0	36.0	36.0	36.0
8	35.16248583913254	36.0	36.0	36.0	36.0	36.0
9	35.26654798511086	36.0	36.0	36.0	36.0	36.0
10	35.161622700544854	36.0	36.0	36.0	36.0	36.0
11	35.24243405081729	36.0	36.0	36.0	36.0	36.0
12	35.26077574580569	36.0	36.0	36.0	36.0	36.0
13	35.21853590117063	36.0	36.0	36.0	36.0	36.0
14	35.171171171171174	36.0	36.0	36.0	36.0	36.0
15	35.182284080487676	36.0	36.0	36.0	36.0	36.0
16	35.187840535145924	36.0	36.0	36.0	36.0	36.0
17	35.15466364568161	36.0	36.0	36.0	36.0	36.0
18	35.1870852888817	36.0	36.0	36.0	36.0	36.0
19	35.13745482008955	36.0	36.0	36.0	36.0	36.0
20	35.1542860225495	36.0	36.0	36.0	36.0	36.0
21	35.09872147596698	36.0	36.0	36.0	36.0	36.0
22	35.15191239143335	36.0	36.0	36.0	36.0	36.0
23	35.03452554350758	36.0	36.0	36.0	36.0	36.0
24	35.00981820143497	36.0	36.0	36.0	36.0	36.0
25	35.03382424340508	36.0	36.0	36.0	36.0	36.0
26	34.99519879160598	36.0	36.0	36.0	36.0	36.0
27	34.97254140367913	36.0	36.0	36.0	36.0	36.0
28	35.00577223930517	36.0	36.0	36.0	36.0	36.0
29	34.96444947941954	36.0	36.0	36.0	36.0	36.0
30	34.912984841128555	36.0	36.0	36.0	32.0	36.0
31	34.91001780223337	36.0	36.0	36.0	32.0	36.0
32	34.90187193181205	36.0	36.0	36.0	32.0	36.0
33	34.90947834061607	36.0	36.0	36.0	32.0	36.0
34	34.85337433241625	36.0	36.0	36.0	32.0	36.0
35	34.89458919997842	36.0	36.0	36.0	32.0	36.0
36	34.84274693855532	36.0	36.0	36.0	32.0	36.0
37	34.85256513999029	36.0	36.0	36.0	32.0	36.0
38	34.89253924583266	36.0	36.0	36.0	32.0	36.0
39	34.864649080217944	36.0	36.0	36.0	32.0	36.0
40	34.7827588067109	36.0	36.0	36.0	32.0	36.0
41	34.79910449371527	36.0	36.0	36.0	32.0	36.0
42	34.79085073097049	36.0	36.0	36.0	32.0	36.0
43	34.788692884501266	36.0	36.0	36.0	32.0	36.0
44	34.77979176781572	36.0	36.0	36.0	32.0	36.0
45	34.74623725521929	36.0	36.0	36.0	32.0	36.0
46	34.69380158601715	36.0	36.0	36.0	32.0	36.0
47	34.68322813831796	36.0	36.0	36.0	32.0	36.0
48	34.708097318875765	36.0	36.0	36.0	32.0	36.0
49	34.64616712520904	36.0	36.0	36.0	32.0	36.0
50	34.2683821546097	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	5.0
24	9.0
25	23.0
26	35.0
27	84.0
28	153.0
29	238.0
30	319.0
31	494.0
32	801.0
33	1364.0
34	3210.0
35	11798.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.400064770335185	17.261294327198144	11.426566632482322	26.91207426998435
2	17.01014242555028	18.65558912386707	35.88692274492879	28.447345705653866
3	20.461779144413875	20.04099908291525	28.41883799967632	31.07838377299455
4	15.013216809623994	15.390839941738147	32.702163241085394	36.893780007552465
5	16.615417813022603	34.02384420348492	32.50795705885526	16.852780924637212
6	35.609861358364356	32.92334250418083	16.237794680908454	15.229001456546367
7	32.65900631170092	29.238819657981335	18.320116523709338	19.782057506608407
8	28.305551060042077	32.39467011922102	17.87236338134542	21.42741543939149
9	28.122134110158065	13.891136645627663	17.87236338134542	40.11436586286886
10	18.30393267519016	24.324324324324326	29.664994335653017	27.7067486648325
11	38.67939796083509	19.949290607973243	22.123320925716136	19.247990505475535
12	25.252198306090524	21.799643955332577	26.390462318606033	26.557695419970866
13	31.418244591897288	19.377461293628958	23.15369261477046	26.050601499703298
14	24.60484436532341	17.688946431461403	24.146301990613367	33.55990721260183
15	27.3614932297567	25.1227275179371	20.704536872201544	26.811242380104655
16	28.60225494956034	24.04380428332524	21.43820467173761	25.91573609537681
17	25.71074068080056	25.03641365916815	23.563683443922965	25.689162216108326
18	25.775476074877275	22.856988725252197	23.876571181960404	27.490964017910123
19	26.25020229810649	24.831418244591895	23.23461185736635	25.683767599935265
20	27.14031396666127	23.5852619086152	23.957490424556294	25.316933700167233
21	27.356098613583647	24.29735124345903	23.31553109996224	25.03101904299509
22	27.954901008793225	22.446997896099692	23.342504180827532	26.25559691427955
23	27.107946269622918	24.178669687651723	22.56028483573394	26.15309920699142
24	26.17467767168366	23.326320332308356	23.482764201327075	27.016237794680908
25	26.827426228623835	23.44500188811566	23.941306576037118	25.786265307223395
26	26.25020229810649	23.493553433673195	23.75249500998004	26.503749258240276
27	25.678372983762205	23.53671036305767	23.74170577763392	27.043210875546205
28	27.329125532718347	22.657387926849005	22.67896639154124	27.334520148891407
29	25.48416680153207	23.790257323191454	24.610238981496465	26.115336893780004
30	26.142309974645308	22.274370178561796	24.534714355073636	27.048605491719265
31	26.109942277606947	22.99724874575174	24.103145061228894	26.78966391541242
32	27.562580923608117	23.28981441519206	23.786145878290892	25.361458782908937
33	25.13351675028322	23.515131898365432	24.070777364190537	27.28057398716081
34	26.203064307293918	23.154941735002158	24.255502805351746	26.386491152352182
35	26.552300803797813	22.91632950315585	23.903544262825697	26.627825430220643
36	26.271780762798723	23.89814964665264	22.3930517343691	27.437017856179537
37	27.430667961584117	23.313909571598142	22.979389230603214	26.276033236214523
38	25.980471489453528	22.824621028213844	23.31013648378918	27.884770998543452
39	26.025032369443245	23.403107466551575	23.716012084592144	26.855848079413036
40	26.503749258240276	23.5852619086152	23.31553109996224	26.595457733182283
41	25.73771376166586	22.695150240060418	24.097750445055834	27.46938555321789
42	26.80874022120313	23.701106015646076	22.762341516050714	26.72781224710008
43	26.17351893816769	23.486565231466493	24.99190676594367	25.348009064422143
44	26.228623833414254	22.92711873550197	23.908938878998757	26.93531855208502
45	25.740490963042888	22.384677636903156	25.303479902886433	26.571351497167523
46	25.569224128628466	22.72040574080069	24.695154850544945	27.0152152800259
47	26.708744672816533	22.117926309543076	24.151696606786427	27.021632410853968
48	26.100561070349592	22.939145446698316	24.34721622788088	26.61307725507121
49	25.71074068080056	23.132114150078223	23.908938878998757	27.248206290122457
50	26.368883853913793	23.488158817500135	23.784862707018394	26.358094621567673
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	3.0
4	4.0
5	5.0
6	6.0
7	4.0
8	2.0
9	2.5
10	3.0
11	4.5
12	6.0
13	8.5
14	11.0
15	19.0
16	27.0
17	21.5
18	16.0
19	24.0
20	32.0
21	38.5
22	45.0
23	56.5
24	68.0
25	74.5
26	81.0
27	99.0
28	117.0
29	137.0
30	157.0
31	200.0
32	243.0
33	262.5
34	282.0
35	321.5
36	361.0
37	442.5
38	524.0
39	563.0
40	602.0
41	684.0
42	766.0
43	789.0
44	812.0
45	802.5
46	793.0
47	923.0
48	1053.0
49	1156.0
50	1259.0
51	1331.0
52	1403.0
53	1378.5
54	1354.0
55	1379.0
56	1404.0
57	1456.0
58	1508.0
59	1443.5
60	1379.0
61	1202.5
62	1026.0
63	955.5
64	885.0
65	749.5
66	614.0
67	554.5
68	495.0
69	435.0
70	375.0
71	320.0
72	265.0
73	245.5
74	226.0
75	170.0
76	114.0
77	96.0
78	78.0
79	78.5
80	79.0
81	53.5
82	28.0
83	23.5
84	19.0
85	11.0
86	3.0
87	3.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05394616173059287
2	0.005394616173059287
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.005394616173059287
33	0.0
34	0.005394616173059287
35	0.0
36	0.0
37	0.01618384851917786
38	0.0
39	0.005394616173059287
40	0.0
41	0.0
42	0.010789232346118574
43	0.01618384851917786
44	0.0
45	0.010789232346118574
46	0.01618384851917786
47	0.0
48	0.005394616173059287
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	18537.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.1042239844635
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.4147100900446	54.113394831957706
2	7.136245055962299	9.14926902950855
3	2.1290919801396955	4.094513675351999
4	1.7083228141041822	4.380428332524141
5	0.9761844652023899	3.1288773803743863
6	0.7826306488260539	3.010195824567082
7	0.5049229992426155	2.2657387926849006
8	0.4460153159976437	2.2873172573771376
9	0.4628460826390642	2.670335005664347
>10	1.405369014558613	13.535091978205752
>50	0.033661533282841036	1.3648378917839996
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
ATCCTGCACCTTGTCGTCATGAACCTCGTTGGCTGAACTCCGTCTCCTTA	84	0.45314775853698014	No Hit
GTGCAGGATCGTGCTGGCAAAGAGGAAGTCAGCAAAGGCTCGCTCCGGAG	65	0.3506500512488536	No Hit
CCTTTATATACTGCTGACCGGGGCGCTGCAGTTCGGCTACCGTCTCCTCG	53	0.2859146571721422	No Hit
GTTCTGATAAGAGTGTTAAAACGTTTTCCAAAAGTCCAGAGTTGTTGGCC	51	0.27512542482602365	No Hit
GCCGTACACTGTGGAGTTGGTCCGATAGAGCGAAGGGAGTTCTCTGGACG	41	0.22117926309543076	No Hit
CCCTTAGGGCACAGCCACCCAGGGAGTCACAGCTGTCCTGTGAGCTGCAT	40	0.21578464692237148	No Hit
CACCAGGAGTCCGTTGGTCTTGAGGACCTCTGTGAACTTGCAGATTTTCT	34	0.18341694988401577	No Hit
CTCTTTGACGTCCCCTGGATCAGCATTCTTGAGCTGGCAGCTTGTTGCAC	31	0.16723310136483788	No Hit
GTACATGGGGTGTTCGTCATGTCGGCGTCTGTGGTGTCCGTCATCTCCCG	30	0.16183848519177862	No Hit
GCCTTAGCCTGCACAGAAGAGCAGCCTTCTGGGGCAACACTGGCTTCAGA	28	0.15104925284566004	No Hit
GTCTTTATTGGGTTCACACCAGGAGTCCGTTGGTCTTGAGGACCTCTGTG	28	0.15104925284566004	No Hit
CTTCAGGGTTATAAATGCTGCTGTTGTCAAACACCTGCTGGATGATCAGC	28	0.15104925284566004	No Hit
CCCTAAGGGTGGTCGTGCAGGCAACATCAGCCCGAGGAGGGTGTGAACCC	28	0.15104925284566004	No Hit
TCTCTGGACGTGCCACCTCACAACCAGTGAGACTCGTAGTCAAGCACGAA	26	0.14026002049954148	No Hit
GGTTTAAACCGCCACTCGTCGGCCTCCAGCTCTTCCACCAGACCAGCCAG	25	0.13486540432648217	No Hit
GTACATGGGGCAGCACCGCCATCAAACTCCCACAGCTCAGGCGGGCGCGC	25	0.13486540432648217	No Hit
GTATTTGCATTGAGAGCCCTAGGCTTCTTTCTCATCTGGGAAATTCCATG	25	0.13486540432648217	No Hit
GTTTAAGCGTGGATGCCACTTGATAGCTAAACACCGACTCACAGAGGAAT	24	0.1294707881534229	No Hit
AATCTGAAGAAGCCATGTGCAACAAGCTGCCAGCTCAAGAATGCTGATCC	24	0.1294707881534229	No Hit
CGCTTAAACAGGTGAAGCATGATCAGCAAGTCGCTCGGATGGAAAAACTG	24	0.1294707881534229	No Hit
GCCCTGGAGCGCCTCAAGGTGTTGGATGGGATCCCTCCACCCTATGACAA	24	0.1294707881534229	No Hit
ATCCAAGACCCTCCTCTTGTGTTTGCCACTGTTCTGATAAGAGTGTTAAA	22	0.11868155580730431	No Hit
ATATACTGCTGACCGGGGCGCTGCAGTTCGGCTACCGTCTCCTCGTGGGC	21	0.11328693963424503	No Hit
GTGTGAACCCCAGCAGCTGCTCGATGGGTTTAAACCGCCACTCGTCGGCC	21	0.11328693963424503	No Hit
TGCTTCACCTGTTTAAGCGTGGATGCCACTTGATAGCTAAACACCGACTC	20	0.10789232346118574	No Hit
GTACATGGGGGAGTGACATCGTCTTTAAACCCCGCGTGGCAATCCCTGAC	20	0.10789232346118574	No Hit
GTCTTGAGGACCTCTGTGAACTTGCAGATTTTCTTCTCCACATTCTTTTC	20	0.10789232346118574	No Hit
GGCATAGGGTCACTTGACGAAGGTTCAGGAGCACAGGGAGAGCTTTGTGG	20	0.10789232346118574	No Hit
CCCTATGACAAGAAAAAGCGGATGGTGGTCCCTGCTGCTCTCAAGGTTGT	20	0.10789232346118574	No Hit
CCTCAAGATCATCCAACTTTTGGATGATTATCCAAAATGCTTCATTGTGG	20	0.10789232346118574	No Hit
TACCTGGGGCGTCTGGCGCATGAGGTCGGGTGGAAGTACCAGGCAGTGAC	20	0.10789232346118574	No Hit
GTTCAGCAGTGTGGCCTCGCTGGCTCCCACCTTGTCTCCAGTCTTTATCA	20	0.10789232346118574	No Hit
CTGCTGAACATGCTGAACATCTCCCCCTTCTCCTTCGGGCTGATCATCCA	19	0.10249770728812645	No Hit
ACCCTGAAGTGCTCGACATCACAGAGCAGGCCCTGCACTCTCGCTTTCTG	19	0.10249770728812645	No Hit
GTCAAAGAGTGAGCGCTCCGCCAACCTCCAACTCCACGGTAAGGAGACGG	19	0.10249770728812645	No Hit
GGTGAAGCATGATCAGCAAGTCGCTCGGATGGAAAAACTGGCTGGTCTGG	19	0.10249770728812645	No Hit
CCCATATCCTCATCTGATTCCTCCGACTCTTCCTTTGCTTCAGCTTTGGC	19	0.10249770728812645	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.005394616173059287	0.0
29	0.0	0.0	0.0	0.010789232346118574	0.0
30	0.0	0.0	0.0	0.026973080865296435	0.0
31	0.0	0.0	0.0	0.03776231321141501	0.0
32	0.0	0.0	0.0	0.05394616173059287	0.0
33	0.0	0.0	0.0	0.09170847494200787	0.0
34	0.0	0.0	0.0	0.11328693963424502	0.0
35	0.0	0.0	0.0	0.14026002049954145	0.0
36	0.0	0.0	0.0	0.19420618223013433	0.0
37	0.0	0.0	0.0	0.3074931218643793	0.0
38	0.0	0.0	0.0	0.4369639100178022	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCTGCC	45	0.0013469217	24.444443	2
>>END_MODULE