FastQCFastQC Report
Thu 2 Feb 2017
SRR4062248_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062248_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092615
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38490.1839325437311689No Hit
TATCAACGCAGAGTACTTTTTTTTT23870.11406780511465318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9900.014.9626471
GTATTAC1650.013.2361881
GTACTAA751.4863412E-512.6607021
ACGGTAT1457.2759576E-1212.4504569
CGTGTCA701.0892212E-412.21671810
GCGGTCG1800.012.1410149
CGGCGTC1501.4551915E-1112.03399914
GTATCAA26000.012.0154931
CGGTCGG1552.5465852E-1111.6474810
TCTATAC1406.82121E-1011.5335933
TATACTG1900.011.5006375
CGAGACT752.0761671E-411.3989944
TCGGCGT1604.5474735E-1111.28187613
GCGTTAT1105.001075E-711.2219861
CGTTCGC855.3216543E-511.17709115
GACCGCG2650.011.1143557
GACCGAT600.005872290611.0844787
CGTCTTA600.005874383311.08394815
GTAATAC1201.2791497E-711.0812943
TTATACT1658.0035534E-1110.9384294
Produced by FastQC (version 0.11.5)