##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062248_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2092615
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3007457176786	32.0	32.0	32.0	32.0	32.0
2	30.869164179746395	32.0	32.0	32.0	32.0	32.0
3	30.893732483041553	32.0	32.0	32.0	32.0	32.0
4	30.89968197685671	32.0	32.0	32.0	32.0	32.0
5	30.81694291592099	32.0	32.0	32.0	32.0	32.0
6	34.485226857305335	36.0	36.0	36.0	32.0	36.0
7	34.42515082803096	36.0	36.0	36.0	32.0	36.0
8	34.38510189404167	36.0	36.0	36.0	32.0	36.0
9	34.522709146211795	36.0	36.0	36.0	32.0	36.0
10	34.22410285695171	36.0	36.0	36.0	32.0	36.0
11	34.501225500151726	36.0	36.0	36.0	32.0	36.0
12	34.34014665860658	36.0	36.0	36.0	32.0	36.0
13	34.41583807819403	36.0	36.0	36.0	32.0	36.0
14	34.320531010243165	36.0	36.0	36.0	32.0	36.0
15	34.27390800505587	36.0	36.0	36.0	32.0	36.0
16	34.28178379682837	36.0	36.0	36.0	32.0	36.0
17	34.218484527732045	36.0	36.0	36.0	32.0	36.0
18	34.22584899754613	36.0	36.0	36.0	32.0	36.0
19	34.22865027728464	36.0	36.0	36.0	32.0	36.0
20	34.21747239697699	36.0	36.0	36.0	32.0	36.0
21	34.197970481908996	36.0	36.0	36.0	32.0	36.0
22	34.174002862447225	36.0	36.0	36.0	32.0	36.0
23	34.14361170114904	36.0	36.0	36.0	32.0	36.0
24	34.11851439466887	36.0	36.0	36.0	32.0	36.0
25	33.7735670441051	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	39.0
5	177.0
6	387.0
7	102.0
8	380.0
9	314.0
10	166.0
11	34.0
12	84.0
13	88.0
14	339.0
15	526.0
16	807.0
17	977.0
18	1261.0
19	1645.0
20	2543.0
21	3560.0
22	5431.0
23	7975.0
24	11391.0
25	15861.0
26	22132.0
27	28594.0
28	38073.0
29	51298.0
30	67588.0
31	92651.0
32	133264.0
33	191607.0
34	430392.0
35	982927.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.68106367859496	16.88116193018498	11.734565874680479	25.703208516539576
2	17.052484696145008	18.557160674946598	37.342075207515975	27.048279421392422
3	19.848380295555206	21.941418443241055	27.540088808011454	30.67011245319229
4	13.320219005452197	14.796154404961268	34.07536341599566	37.80826317359088
5	15.398224504439217	35.780093244349814	33.05035605995058	15.771326191260385
6	36.15935511234991	33.78511427695683	15.870909620380639	14.184620990312627
7	31.660995250936825	29.662341464674807	19.191008784834	19.48565449955437
8	28.718379523694935	33.07617714020817	18.131903617616427	20.073539718480465
9	26.693392353755087	15.009635791943445	18.20576195479311	40.09120989950836
10	16.665956877687726	26.83251165290075	30.314959687973577	26.18657178143795
11	37.27952573717137	21.53386966098994	21.26765193863258	19.918952663206106
12	24.83407773057573	23.374392415856953	27.876472485374435	23.91505736819288
13	29.62662225031473	19.48236320894426	24.713900668972656	26.17711387176836
14	23.663782854337896	19.1545867255964	24.52017129682567	32.661459123240036
15	25.361399949277203	26.854516482517383	21.8198479273037	25.964235640901716
16	26.015651800311083	25.963357926923496	22.88433485111556	25.136655421649856
17	24.351829833411404	26.160012516752403	24.450250933134416	25.03790671670178
18	24.92063081992812	25.459566210832097	25.198877445183122	24.42092552405666
19	25.728112613858034	24.982659999177248	24.983855861302953	24.30537152566177
20	25.899859374551575	24.551385686816605	24.70836960577043	24.84038533286139
21	26.224165227747775	24.31594354017095	24.636220577895333	24.82367065418594
22	26.05625111222094	24.060807151891588	24.84376285631732	25.03917887957015
23	24.96250382614019	24.3073729721457	25.16213269054178	25.56799051117233
24	24.861335389466273	24.76304088286165	25.06122480216736	25.314398925504722
25	25.03629158585262	24.513118780507053	25.032943432956827	25.417646200683503
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	179.0
1	179.0
2	426.5
3	674.0
4	674.0
5	674.0
6	1322.0
7	1970.0
8	1970.0
9	1970.0
10	2434.0
11	2898.0
12	2898.0
13	2898.0
14	3864.0
15	4830.0
16	4830.0
17	4830.0
18	8290.0
19	11750.0
20	11750.0
21	11750.0
22	17953.5
23	24157.0
24	24157.0
25	24157.0
26	35412.5
27	46668.0
28	46668.0
29	46668.0
30	59900.5
31	73133.0
32	73133.0
33	73133.0
34	92547.5
35	111962.0
36	111962.0
37	111962.0
38	136228.5
39	160495.0
40	160495.0
41	160495.0
42	187231.0
43	213967.0
44	213967.0
45	213967.0
46	241288.0
47	268609.0
48	268609.0
49	268609.0
50	282979.0
51	297349.0
52	297349.0
53	297349.0
54	284701.0
55	272053.0
56	272053.0
57	272053.0
58	251204.0
59	230355.0
60	230355.0
61	230355.0
62	199537.5
63	168720.0
64	168720.0
65	168720.0
66	136644.0
67	104568.0
68	104568.0
69	104568.0
70	78761.5
71	52955.0
72	52955.0
73	52955.0
74	38945.5
75	24936.0
76	24936.0
77	24936.0
78	18620.0
79	12304.0
80	12304.0
81	12304.0
82	8397.5
83	4491.0
84	4491.0
85	4491.0
86	3204.0
87	1917.0
88	1917.0
89	1917.0
90	1279.5
91	642.0
92	642.0
93	642.0
94	424.0
95	206.0
96	206.0
97	206.0
98	516.5
99	827.0
100	827.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0013380387696733513
2	2.3893549458452702E-4
3	5.256580880859595E-4
4	0.011803413432475634
5	0.033068672450498535
6	0.06384356415298562
7	0.11029262430021768
8	0.11067492109155291
9	0.11903766340201137
10	0.1334215801759999
11	0.14202325798104287
12	0.13289592208791393
13	0.12873844448214317
14	0.116696095555083
15	0.13499855444025777
16	0.11965889568793112
17	0.12501105076662455
18	0.11387665671898557
19	0.0990626560547449
20	0.09342377838255006
21	0.0919901654150429
22	0.10527497891394261
23	0.08291061662083088
24	0.09361492677821769
25	0.09127335893128932
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2092615.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.34134952276742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.52475515198242	37.74860152833824
2	14.205713114270521	14.586809644398475
3	5.088991041974241	7.838270034126955
4	2.2758569883008164	4.6738227640074
5	1.2658880045104843	3.2496199248125675
6	0.7748370957049412	2.3868709292276025
7	0.5345533075538147	1.921128174117025
8	0.3807600044535242	1.5638985978351092
9	0.26886710159561306	1.2423599854374408
>10	1.531431167318482	14.182406414014523
>50	0.09211584243244346	3.242427037808774
>100	0.050713177477219915	4.9098641306002895
>500	0.004395696847311132	1.563057059581022
>1k	0.0011223055780368802	0.890863775694477
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3849	0.1839325437311689	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2387	0.11406780511465318	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.911483956676216E-4
2	0.0	0.0	0.0	0.0	1.911483956676216E-4
3	0.0	0.0	0.0	0.0	1.911483956676216E-4
4	0.0	0.0	0.0	0.0	1.911483956676216E-4
5	0.0	0.0	0.0	0.0	2.3893549458452702E-4
6	0.0	0.0	0.0	0.0	2.3893549458452702E-4
7	0.0	0.0	0.0	0.0	2.3893549458452702E-4
8	0.0	0.0	0.0	0.0	2.3893549458452702E-4
9	0.0	0.0	0.0	0.0	2.3893549458452702E-4
10	0.0	0.0	0.0	0.0	2.3893549458452702E-4
11	0.0	0.0	0.0	0.0	2.3893549458452702E-4
12	0.0	0.0	0.0	0.0	7.645935826704864E-4
13	0.0	0.0	0.0	0.0	9.557419783381081E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	990	0.0	14.962647	1
GTATTAC	165	0.0	13.236188	1
GTACTAA	75	1.4863412E-5	12.660702	1
ACGGTAT	145	7.2759576E-12	12.450456	9
CGTGTCA	70	1.0892212E-4	12.216718	10
GCGGTCG	180	0.0	12.141014	9
CGGCGTC	150	1.4551915E-11	12.033999	14
GTATCAA	2600	0.0	12.015493	1
CGGTCGG	155	2.5465852E-11	11.64748	10
TCTATAC	140	6.82121E-10	11.533593	3
TATACTG	190	0.0	11.500637	5
CGAGACT	75	2.0761671E-4	11.398994	4
TCGGCGT	160	4.5474735E-11	11.281876	13
GCGTTAT	110	5.001075E-7	11.221986	1
CGTTCGC	85	5.3216543E-5	11.177091	15
GACCGCG	265	0.0	11.114355	7
GACCGAT	60	0.0058722906	11.084478	7
CGTCTTA	60	0.0058743833	11.083948	15
GTAATAC	120	1.2791497E-7	11.081294	3
TTATACT	165	8.0035534E-11	10.938429	4
>>END_MODULE