FastQCFastQC Report
Thu 2 Feb 2017
SRR4062248_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062248_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2092615
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA10750.028.863871
GTATCAA28700.028.3702871
TACCGTA1551.8189894E-1221.2891437
TCAACGC41150.019.6732734
CGCAATA2800.019.64317736
TATCAAC42100.019.4397472
CAACGCA41950.019.2980965
ACCGTAT1605.2750693E-1119.2489348
ATCAACG42700.019.0106563
ATACGAA2900.018.96673240
ATACGCG708.1225345E-418.8583539
AACGCAG43550.018.5890966
CGACGCT600.00741112718.33450941
TTTTACG851.4312871E-418.11794324
TACGAAT3050.018.03394341
CGAATGC2950.017.90073443
CTAGCGG3000.017.60028829
CCGTATG1854.2382453E-1016.6477289
ACGCGTA800.001988152716.50105941
GCGCAAT3200.016.50026935