##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062248_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2092615
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.30432783861341	32.0	32.0	32.0	32.0	32.0
2	31.480319600117557	32.0	32.0	32.0	32.0	32.0
3	31.59648764822961	32.0	32.0	32.0	32.0	32.0
4	31.669380655304487	32.0	32.0	32.0	32.0	32.0
5	31.648162227643404	32.0	32.0	32.0	32.0	32.0
6	35.30773744812113	36.0	36.0	36.0	36.0	36.0
7	35.31064816031616	36.0	36.0	36.0	36.0	36.0
8	35.27149714591552	36.0	36.0	36.0	36.0	36.0
9	35.34783894791923	36.0	36.0	36.0	36.0	36.0
10	35.24341362362403	36.0	36.0	36.0	36.0	36.0
11	35.349424045990304	36.0	36.0	36.0	36.0	36.0
12	35.281314049646014	36.0	36.0	36.0	36.0	36.0
13	35.30872759681069	36.0	36.0	36.0	36.0	36.0
14	35.27683114189662	36.0	36.0	36.0	36.0	36.0
15	35.253921050933876	36.0	36.0	36.0	36.0	36.0
16	35.26872788353328	36.0	36.0	36.0	36.0	36.0
17	35.24748269509681	36.0	36.0	36.0	36.0	36.0
18	35.245577901333974	36.0	36.0	36.0	36.0	36.0
19	35.239045404912034	36.0	36.0	36.0	36.0	36.0
20	35.24175397767865	36.0	36.0	36.0	36.0	36.0
21	35.23603147258335	36.0	36.0	36.0	36.0	36.0
22	35.21072055777102	36.0	36.0	36.0	36.0	36.0
23	35.193027384397034	36.0	36.0	36.0	36.0	36.0
24	35.177403392406156	36.0	36.0	36.0	36.0	36.0
25	35.163324357323255	36.0	36.0	36.0	36.0	36.0
26	35.12543253297907	36.0	36.0	36.0	36.0	36.0
27	35.11182133359457	36.0	36.0	36.0	36.0	36.0
28	35.09105927272814	36.0	36.0	36.0	36.0	36.0
29	35.08003431113702	36.0	36.0	36.0	36.0	36.0
30	35.063804856602864	36.0	36.0	36.0	36.0	36.0
31	35.06111492080483	36.0	36.0	36.0	36.0	36.0
32	35.03430492469948	36.0	36.0	36.0	36.0	36.0
33	35.017843224864585	36.0	36.0	36.0	36.0	36.0
34	35.01340093614927	36.0	36.0	36.0	36.0	36.0
35	34.99848992767423	36.0	36.0	36.0	36.0	36.0
36	34.97726576556127	36.0	36.0	36.0	36.0	36.0
37	34.962845530592105	36.0	36.0	36.0	36.0	36.0
38	34.95030762944928	36.0	36.0	36.0	36.0	36.0
39	34.93705578904863	36.0	36.0	36.0	36.0	36.0
40	34.92721738112362	36.0	36.0	36.0	36.0	36.0
41	34.89795399535987	36.0	36.0	36.0	36.0	36.0
42	34.87348269987552	36.0	36.0	36.0	36.0	36.0
43	34.86045498096879	36.0	36.0	36.0	32.0	36.0
44	34.82760087259243	36.0	36.0	36.0	32.0	36.0
45	34.809825983279296	36.0	36.0	36.0	32.0	36.0
46	34.79306131323727	36.0	36.0	36.0	32.0	36.0
47	34.7646681305448	36.0	36.0	36.0	32.0	36.0
48	34.737828984309104	36.0	36.0	36.0	32.0	36.0
49	34.72619569294878	36.0	36.0	36.0	32.0	36.0
50	34.32433820841388	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	20.0
21	50.0
22	126.0
23	396.0
24	969.0
25	2295.0
26	4584.0
27	8664.0
28	14686.0
29	23625.0
30	35276.0
31	52213.0
32	79640.0
33	136258.0
34	320231.0
35	1413579.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.379304394132994	17.361942690459355	12.132015681415439	26.12673723399221
2	16.51039605758405	18.95266195274224	37.1052375026523	27.431704487021413
3	19.30518314697108	22.236499986380636	27.9133368918895	30.544979974758785
4	13.05147533180988	15.158218381411956	34.173765443603074	37.61654084317509
5	15.031582508766547	36.03731983060421	32.988692611250805	15.942405049378433
6	36.339321383992825	33.86303815518596	15.688813788700436	14.108826672120776
7	31.662728213264263	29.671774311089234	19.172088511264615	19.493408964381885
8	28.736867507878898	33.19205873990199	18.1253121094898	19.945761642729313
9	26.90480570960258	14.753741132506457	18.177400047309227	40.16405311058174
10	16.644740331824377	26.810117303103826	30.480313368979356	26.064828996092444
11	37.50646917851588	21.34176616338887	21.18703153709593	19.964733120999323
12	24.84064197188685	23.296210721991383	27.982357003079876	23.880790303041888
13	29.68964668608416	19.347514951388572	24.805996325172096	26.156842037355176
14	23.66689524828982	18.94825373993783	24.694747958893537	32.69010305287882
15	25.384459157561235	26.835848925865484	21.816005333040238	25.96368658353304
16	26.021982223071777	25.95015769855682	22.96932046258243	25.058539615788973
17	24.42938619860796	26.036131825491072	24.56672632089515	24.967755655005817
18	25.069757284313916	25.199117084716573	25.397003650459975	24.334121980509536
19	25.779132807515953	24.879970754295464	25.102132977160156	24.238763461028427
20	26.046946158147005	24.338792225976174	24.882180626108067	24.732080989768757
21	26.356114239838668	24.122306300967928	24.76556843948839	24.75601101970501
22	26.135863500930657	23.953235544999917	25.02830190933354	24.882599044735894
23	25.02873199322379	24.098269390212725	25.370218602083995	25.50278001447949
24	24.946131323446696	24.608654637867254	25.172931712820816	25.27228232586523
25	25.24349974433841	24.27255914862301	25.17932151714844	25.304619589890137
26	24.919992067303674	24.879802925062304	25.35007801281178	24.850126994822244
27	25.220610090958356	24.608645870268937	24.969487806113523	25.20125623265918
28	25.16787856544827	24.360599144407104	25.28591444835667	25.185607841787956
29	24.967576251958086	24.752532970913723	25.269879317710682	25.01001145941751
30	25.134330754718064	24.715807542401876	25.1353820804398	25.014479622440263
31	25.267788235237898	24.524790870708994	24.886206426917646	25.321214467135462
32	25.31296055234742	24.636719175607713	24.691531475736358	25.358788796308506
33	24.99304696049431	24.514744744601238	25.01999894868131	25.47220934622314
34	25.480801625156086	24.503691342679605	25.065194048166894	24.95031298399741
35	25.5260553482911	24.4397049989893	25.278373394284536	24.75586625843507
36	24.998172138225534	24.742701574923935	25.03506388436636	25.224062402484172
37	25.68661914619903	24.370869915109253	24.98824413210155	24.954266806590166
38	25.0876176877341	24.45233569114992	25.086088499838	25.37395812127798
39	25.60698500475725	24.4751856186356	24.747335487912867	25.17049388869429
40	25.542958834143803	24.61813201516959	24.91317055081506	24.925738599871547
41	24.86941994259734	24.961220231808873	25.18534483170584	24.984014993887946
42	25.66729892382472	24.613871521331152	24.87021523983478	24.848614315009346
43	25.306355740639674	24.304492898931517	24.9952927165661	25.393858643862703
44	25.0535118031817	24.755212472563105	24.86536626254642	25.325909461708772
45	25.161333177862183	24.751405452832028	24.970801370949705	25.116459998356085
46	25.194988901128045	24.624920252992155	24.87122202283811	25.308868823041685
47	25.162920035496665	24.72016748614517	25.080725282675882	25.03618719568229
48	25.503196621110796	25.089103005846535	24.46072251570115	24.946977857341523
49	25.215898626210436	24.842188868706014	24.533137589705873	25.40877491537768
50	25.182442858624288	25.12156087266729	24.71521901505945	24.980777253648974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	40.0
1	66.0
2	92.0
3	228.5
4	365.0
5	399.0
6	433.0
7	325.0
8	217.0
9	318.5
10	420.0
11	730.5
12	1041.0
13	1961.5
14	2882.0
15	3732.5
16	4583.0
17	4307.5
18	4032.0
19	3783.5
20	3535.0
21	4100.0
22	4665.0
23	5538.5
24	6412.0
25	8387.0
26	10362.0
27	12843.5
28	15325.0
29	18305.5
30	21286.0
31	24751.5
32	28217.0
33	32739.5
34	37262.0
35	43415.5
36	49569.0
37	57135.0
38	64701.0
39	73699.5
40	82698.0
41	91040.5
42	99383.0
43	106283.0
44	113183.0
45	123592.5
46	134002.0
47	143462.0
48	152922.0
49	159847.0
50	166772.0
51	168166.5
52	169561.0
53	169761.0
54	169961.0
55	167906.5
56	165852.0
57	158531.5
58	151211.0
59	139848.5
60	128486.0
61	112645.0
62	96804.0
63	84061.5
64	71319.0
65	59945.0
66	48571.0
67	40502.0
68	32433.0
69	27372.0
70	22311.0
71	17176.5
72	12042.0
73	10124.0
74	8206.0
75	6250.5
76	4295.0
77	3647.0
78	2999.0
79	2470.0
80	1941.0
81	1467.0
82	993.0
83	814.5
84	636.0
85	495.5
86	355.0
87	238.0
88	121.0
89	84.0
90	47.0
91	33.0
92	19.0
93	17.0
94	15.0
95	14.5
96	14.0
97	15.5
98	17.0
99	12.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03402441442883665
2	0.0043486260014383915
3	2.867225935014324E-4
4	4.77870989169054E-5
5	4.77870989169054E-5
6	1.433612967507162E-4
7	0.0
8	0.0
9	0.0
10	9.55741978338108E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.168064837535811E-4
17	0.0
18	9.55741978338108E-5
19	0.0
20	2.3893549458452702E-4
21	0.0
22	0.0
23	0.0
24	0.0012424645718395405
25	2.3893549458452702E-4
26	4.7787098916905404E-4
27	4.3008389025214863E-4
28	0.0014814000664240675
29	6.212322859197703E-4
30	9.079548794212026E-4
31	4.7787098916905404E-4
32	9.079548794212026E-4
33	2.3893549458452702E-4
34	7.645935826704864E-4
35	5.734451870028648E-4
36	2.867225935014324E-4
37	0.0020548452534269327
38	4.77870989169054E-5
39	8.601677805042973E-4
40	3.345096924183378E-4
41	0.0013858258685902566
42	0.005352155078693405
43	0.005399942177610311
44	0.004109690506853865
45	0.0024371420447621755
46	0.0028672259350143244
47	0.0011468903740057296
48	0.004444200199272203
49	0.003918542111186243
50	0.002007058154510027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2092615.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.43625677140881
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.95083841867589	38.25268895011243
2	14.933696458670884	15.661342841062895
3	5.12299683739751	8.058923328146731
4	2.231789144898523	4.681066746461673
5	1.2189756327473629	3.1959259638415625
6	0.7293109537410658	2.2945401861940544
7	0.5293154427898844	1.9428724329841625
8	0.3604343900683311	1.5119864181495304
9	0.2807460989846296	1.324914708053645
>10	1.5073219354503655	13.983134479836382
>50	0.08751095416784456	3.106113159516299
>100	0.0431129129911774	4.244849516639803
>500	0.0028482651606482104	1.0285560014356516
>1k	0.0011025542557347866	0.7130852675651803
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.911483956676216E-4
2	0.0	0.0	0.0	0.0	1.911483956676216E-4
3	0.0	0.0	0.0	0.0	1.911483956676216E-4
4	0.0	0.0	0.0	0.0	1.911483956676216E-4
5	0.0	0.0	0.0	0.0	1.911483956676216E-4
6	0.0	0.0	0.0	0.0	2.867225935014324E-4
7	0.0	0.0	0.0	0.0	2.867225935014324E-4
8	0.0	0.0	0.0	0.0	2.867225935014324E-4
9	0.0	0.0	0.0	0.0	2.867225935014324E-4
10	0.0	0.0	0.0	0.0	3.822967913352432E-4
11	0.0	0.0	0.0	0.0	4.3008389025214863E-4
12	0.0	0.0	0.0	0.0	0.0010035290772550136
13	0.0	0.0	0.0	0.0	0.001051316176171919
14	0.0	0.0	0.0	0.0	0.001051316176171919
15	0.0	0.0	0.0	1.911483956676216E-4	0.001194677472922635
16	0.0	0.0	0.0	2.867225935014324E-4	0.0013858258685902566
17	0.0	0.0	0.0	2.867225935014324E-4	0.0014336129675071622
18	0.0	0.0	0.0	3.822967913352432E-4	0.0014814000664240675
19	0.0	0.0	0.0	3.822967913352432E-4	0.0014814000664240675
20	0.0	0.0	0.0	3.822967913352432E-4	0.0017681226599254999
21	0.0	0.0	0.0	6.690193848366756E-4	0.002007058154510027
22	0.0	0.0	0.0	9.557419783381081E-4	0.0020548452534269322
23	0.0	0.0	0.0	0.001290251670756446	0.002102632352343838
24	0.0	0.0	0.0	0.0018636968577593108	0.002102632352343838
25	0.0	0.0	0.0	0.002150419451260743	0.002150419451260743
26	0.0	0.0	0.0	0.0027716517371805132	0.00238935494584527
27	0.0	0.0	0.0	0.003249522726349567	0.002484929143679081
28	0.0	0.0	0.0	0.0038229679133524323	0.002484929143679081
29	0.0	0.0	0.0	0.005830026067862459	0.002580503341512892
30	0.0	0.0	0.0	0.00874503910179369	0.002580503341512892
31	0.0	0.0	0.0	0.014575065169656148	0.0026282904404297974
32	0.0	0.0	0.0	0.020309517039684796	0.0026282904404297974
33	0.0	0.0	0.0	0.026665201195633214	0.0026282904404297974
34	0.0	0.0	0.0	0.033833266033169024	0.002723864638263608
35	0.0	0.0	0.0	0.04052345988153578	0.002723864638263608
36	0.0	0.0	0.0	0.05141891843459021	0.0027716517371805132
37	0.0	0.0	0.0	0.0681444030555071	0.0027716517371805132
38	0.0	0.0	0.0	0.08897957818327785	0.0028672259350143244
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1075	0.0	28.86387	1
GTATCAA	2870	0.0	28.370287	1
TACCGTA	155	1.8189894E-12	21.289143	7
TCAACGC	4115	0.0	19.673273	4
CGCAATA	280	0.0	19.643177	36
TATCAAC	4210	0.0	19.439747	2
CAACGCA	4195	0.0	19.298096	5
ACCGTAT	160	5.2750693E-11	19.248934	8
ATCAACG	4270	0.0	19.010656	3
ATACGAA	290	0.0	18.966732	40
ATACGCG	70	8.1225345E-4	18.85835	39
AACGCAG	4355	0.0	18.589096	6
CGACGCT	60	0.007411127	18.334509	41
TTTTACG	85	1.4312871E-4	18.117943	24
TACGAAT	305	0.0	18.033943	41
CGAATGC	295	0.0	17.900734	43
CTAGCGG	300	0.0	17.600288	29
CCGTATG	185	4.2382453E-10	16.647728	9
ACGCGTA	80	0.0019881527	16.501059	41
GCGCAAT	320	0.0	16.500269	35
>>END_MODULE