FastQCFastQC Report
Thu 2 Feb 2017
SRR4062247_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062247_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62379
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT1150.18435691498741566No Hit
TATCAACGCAGAGTACTTTTTTTTT1060.16992898251013963No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT740.11862966703538048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA653.2484804E-614.6153861
TCAACGC1304.0197873E-48.0384624
ATCAACG1304.0197873E-48.0384623
TATCAAC1356.027577E-47.74074032
AACGCAG1408.890668E-47.4642866
AGAGTAC1408.890668E-47.46428611
CAGAGTA1450.00129146077.206896310
GCAGAGT1450.00129146077.20689639
CAACGCA1450.00129146077.20689635
ACGCAGA1550.0026133566.74193577
CGCAGAG1550.0026133566.74193578
Produced by FastQC (version 0.11.5)