FastQCFastQC Report
Thu 2 Feb 2017
SRR4062247_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062247_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62379
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA640.10259863094951828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA250.002335774235.21
GCATGGC300.005703857629.3333328
GATTAAG450.001383414324.4444451
TATCAAC808.815678E-519.2500021
ATCAACG808.815678E-519.2500022
TCAACGC851.4034755E-418.1176453
CAACGCA953.283096E-416.2105264
AACGCAG1004.8508577E-415.4000015
ACGCAGA1150.001397611113.3913036
CGCAGAG1150.001397611113.3913037
AGAGTAC1250.002616683712.3210
GCAGAGT1350.004653911611.4074078
AGTACAT1350.004653911611.40740712
GAGTACA1400.006102582411.011
CAGAGTA1400.006102582411.09
GTACATG1900.00543492579.26315813
Produced by FastQC (version 0.11.5)