##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062247_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	62379
Sequences flagged as poor quality	0
Sequence length	50
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	28.96067586848138	32.0	32.0	32.0	21.0	32.0
2	28.602782987864504	32.0	32.0	32.0	14.0	32.0
3	30.341877875567096	32.0	32.0	32.0	21.0	32.0
4	30.944580708251173	32.0	32.0	32.0	32.0	32.0
5	30.638965036310296	32.0	32.0	32.0	32.0	32.0
6	34.19971464755767	36.0	36.0	36.0	32.0	36.0
7	34.20086888215585	36.0	36.0	36.0	32.0	36.0
8	34.049888584299204	36.0	36.0	36.0	32.0	36.0
9	34.417512303820196	36.0	36.0	36.0	32.0	36.0
10	33.94336234950865	36.0	36.0	36.0	32.0	36.0
11	34.58928485548021	36.0	36.0	36.0	32.0	36.0
12	34.2363135029417	36.0	36.0	36.0	32.0	36.0
13	34.45117747960051	36.0	36.0	36.0	32.0	36.0
14	34.30603247887911	36.0	36.0	36.0	32.0	36.0
15	34.17752769361484	36.0	36.0	36.0	32.0	36.0
16	34.25303387357925	36.0	36.0	36.0	32.0	36.0
17	34.201045223552796	36.0	36.0	36.0	32.0	36.0
18	34.2212282979849	36.0	36.0	36.0	32.0	36.0
19	34.209541672678306	36.0	36.0	36.0	32.0	36.0
20	34.2547972875487	36.0	36.0	36.0	32.0	36.0
21	34.25058112505811	36.0	36.0	36.0	32.0	36.0
22	34.24306256913384	36.0	36.0	36.0	32.0	36.0
23	34.12898571634685	36.0	36.0	36.0	32.0	36.0
24	34.114349380400455	36.0	36.0	36.0	32.0	36.0
25	34.09969701341798	36.0	36.0	36.0	32.0	36.0
26	33.979448211737925	36.0	36.0	36.0	32.0	36.0
27	33.92814889626317	36.0	36.0	36.0	32.0	36.0
28	33.922265506019656	36.0	36.0	36.0	32.0	36.0
29	33.87494188749419	36.0	36.0	36.0	32.0	36.0
30	33.86170025168727	36.0	36.0	36.0	32.0	36.0
31	33.823418137514224	36.0	36.0	36.0	32.0	36.0
32	33.769505763157476	36.0	36.0	36.0	32.0	36.0
33	33.69270106927011	36.0	36.0	36.0	32.0	36.0
34	33.717388864842334	36.0	36.0	36.0	32.0	36.0
35	33.71647509578544	36.0	36.0	36.0	32.0	36.0
36	33.6411131951458	36.0	36.0	36.0	27.0	36.0
37	33.63938184324853	36.0	36.0	36.0	27.0	36.0
38	33.56958271213068	36.0	36.0	36.0	27.0	36.0
39	33.56291380111897	36.0	36.0	36.0	27.0	36.0
40	33.532054056653685	36.0	36.0	36.0	27.0	36.0
41	33.492345180269	36.0	36.0	36.0	21.0	36.0
42	33.454848586864166	36.0	36.0	36.0	21.0	36.0
43	33.451209541672675	36.0	36.0	36.0	21.0	36.0
44	33.34853075554273	36.0	36.0	36.0	21.0	36.0
45	33.33165007454432	36.0	36.0	36.0	21.0	36.0
46	33.31489764183459	36.0	36.0	36.0	21.0	36.0
47	33.29067474630885	36.0	36.0	36.0	21.0	36.0
48	33.28568909408615	36.0	36.0	36.0	21.0	36.0
49	33.19282130204075	36.0	36.0	36.0	21.0	36.0
50	32.52674778370926	36.0	32.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	0.0
22	10.0
23	33.0
24	51.0
25	151.0
26	272.0
27	583.0
28	1058.0
29	1772.0
30	2849.0
31	4750.0
32	7674.0
33	12016.0
34	17847.0
35	13312.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.33651857433743	18.362700614067435	13.985666415481553	26.315114396113575
2	18.81081774315074	20.83553760079514	34.417031372737625	25.9366132833165
3	22.675900543452123	21.920838743808012	27.820260023405314	27.583000689334554
4	17.510700716587312	16.830984786546754	33.41509161737123	32.2432228794947
5	22.02023116754036	30.965549303451485	29.432982253643054	17.58123727536511
6	36.00250084162939	33.96014684429055	14.80786803251094	15.229484281569118
7	30.81646066785296	29.415348113948603	18.488593917824907	21.279597300373524
8	28.985716346847497	31.33105692620914	17.523525545456003	22.15970118148736
9	26.5073181679732	13.685695506500586	17.40970518924638	42.39728113627984
10	17.632536590839866	25.35148046618253	29.03541255871367	27.980570384263935
11	37.71461549559948	20.2231520223152	20.30010099552734	21.762131486557976
12	24.607640391798522	22.656663300149088	26.539380240144922	26.19631606790747
13	29.89146988569871	17.93231696564549	23.405312685358854	28.770900463296943
14	23.895862389586238	17.866589717693454	23.358822680709853	34.87872521201045
15	25.832411548758394	25.282547011013325	20.87401208740121	28.011029352827073
16	26.60991679892272	24.56595969797528	22.505971560941983	26.31815194216002
17	25.056509402202664	25.05009698776832	23.612113050866476	26.28128055916254
18	25.765081197197777	24.24854518347521	23.998461020535757	25.987912598791258
19	26.319755045768606	24.279004152038347	23.987239295275653	25.414001506917394
20	26.563426794273713	23.37325061318713	23.697077542121548	26.366245050417607
21	27.220699273794065	23.33958543740682	23.39409096009875	26.045624328700363
22	26.587473348402508	23.67142788438417	23.546385802914443	26.194712964298883
23	25.56789945334167	23.507911316308373	23.867006524631687	27.05718270571827
24	25.40680058354842	23.848533914744216	24.353527742597432	26.391137759109927
25	25.94623190496802	23.358822680709853	24.47297968867728	26.221965725644846
26	26.002340531268537	23.55921063178313	23.915099632889273	26.52334920405906
27	25.951041215793776	23.822119623591274	23.988842398884238	26.23799676173071
28	25.755462575546257	23.684252713252857	24.15075586335145	26.409528847849433
29	25.89813879671043	23.786851344202375	24.020904471055964	26.29410538803123
30	25.71419410689666	23.756773221328032	24.55352848760781	25.975504184167498
31	26.226775036470606	23.39890027092451	24.309463120601485	26.0648615720034
32	26.24761538338223	23.60730374004072	23.50951441991696	26.635566456660094
33	25.45247599352346	23.91670273649786	24.442520720114143	26.18830054986454
34	26.061655364786223	23.573638564260406	24.21007069686914	26.15463537408423
35	26.403116433415093	23.89906859680341	23.697077542121548	26.00073742765995
36	25.381939434745664	23.935939979800892	23.820516519982686	26.86160406547075
37	26.130588819955435	24.04495102518476	23.706696163773064	26.117763991086747
38	26.229981243687778	23.487070969396754	24.064188268487793	26.218759518427674
39	26.114557783869575	23.855784799371584	23.60249442921496	26.427162987543884
40	26.409528847849433	24.028919989098895	23.799676173071067	25.761874989980605
41	26.18349123903878	23.80127927667965	24.105868962311032	25.909360521970537
42	26.503783506476854	23.608439143260227	23.781582659997436	26.10619469026549
43	26.09413575298984	23.57401648016929	24.072589695084805	26.25925807175607
44	25.59917918463536	24.342305657534027	23.733106754092052	26.32540840373856
45	26.234790554513538	24.205261386043382	23.519133041568477	26.040815017874607
46	25.4476666826977	24.381602782987866	24.19724586800045	25.973484666313983
47	25.761874989980605	24.07380689013931	24.335112778338864	25.829205341541222
48	26.46606175254096	24.404437461925678	23.277437558113437	25.852063227419926
49	26.04966574218061	24.223672186863748	23.6401237635667	26.086538307388942
50	26.363038843200435	24.056172750444862	23.92952756536655	25.651260840988154
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	3.5
2	2.0
3	3.5
4	5.0
5	3.5
6	2.0
7	2.0
8	2.0
9	5.0
10	8.0
11	10.5
12	13.0
13	15.0
14	17.0
15	17.5
16	18.0
17	23.0
18	28.0
19	46.5
20	65.0
21	80.0
22	95.0
23	111.0
24	127.0
25	177.5
26	228.0
27	290.0
28	352.0
29	417.5
30	483.0
31	596.5
32	710.0
33	855.0
34	1000.0
35	1185.5
36	1371.0
37	1573.0
38	1775.0
39	2000.5
40	2226.0
41	2426.5
42	2627.0
43	2888.0
44	3149.0
45	3430.0
46	3711.0
47	3961.0
48	4211.0
49	4313.5
50	4416.0
51	4502.5
52	4589.0
53	4620.0
54	4651.0
55	4737.5
56	4824.0
57	4785.5
58	4747.0
59	4354.0
60	3961.0
61	3679.5
62	3398.0
63	2979.5
64	2561.0
65	2270.5
66	1980.0
67	1776.5
68	1573.0
69	1418.5
70	1264.0
71	1004.0
72	744.0
73	643.0
74	542.0
75	421.5
76	301.0
77	268.0
78	235.0
79	196.5
80	158.0
81	123.5
82	89.0
83	72.5
84	56.0
85	39.0
86	22.0
87	16.0
88	10.0
89	7.0
90	4.0
91	5.5
92	7.0
93	4.0
94	1.0
95	2.5
96	4.0
97	4.0
98	4.0
99	6.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.012824828868689785
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0032062072171724462
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0016031036085862231
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.004809310825758669
43	0.0016031036085862231
44	0.0032062072171724462
45	0.0
46	0.0
47	0.0
48	0.0016031036085862231
49	0.0032062072171724462
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	62379.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	87.09341284727232
#Duplication Level	Percentage of deduplicated	Percentage of total
1	93.01097040200264	81.00642844547043
2	4.820718598144603	8.397056701774636
3	1.0362980415255485	2.7076419949021306
4	0.3718156383448682	1.2953077157376682
5	0.20063319098807247	0.8736914666794915
6	0.14173170372551908	0.740633867166835
7	0.09203357384773965	0.561086263005178
8	0.05890148726255338	0.4103945237980731
9	0.042335443969960244	0.33184244697734816
>10	0.21719923428066557	3.312012055339136
>50	0.007362685907819172	0.3639045191490726
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	64	0.10259863094951828	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.001603103608586223
6	0.0	0.0	0.0	0.0	0.001603103608586223
7	0.0	0.0	0.0	0.0	0.001603103608586223
8	0.0	0.0	0.0	0.0	0.001603103608586223
9	0.0	0.0	0.0	0.0	0.001603103608586223
10	0.0	0.0	0.0	0.0	0.001603103608586223
11	0.0	0.0	0.0	0.0	0.001603103608586223
12	0.0	0.0	0.0	0.0	0.003206207217172446
13	0.0	0.0	0.0	0.0	0.003206207217172446
14	0.0	0.0	0.0	0.0	0.003206207217172446
15	0.0	0.0	0.0	0.0	0.003206207217172446
16	0.0	0.0	0.0	0.0	0.003206207217172446
17	0.0	0.0	0.0	0.0	0.003206207217172446
18	0.0	0.0	0.0	0.0	0.003206207217172446
19	0.0	0.0	0.0	0.0	0.003206207217172446
20	0.0	0.0	0.0	0.0	0.003206207217172446
21	0.0	0.0	0.0	0.0	0.003206207217172446
22	0.0	0.0	0.0	0.0	0.003206207217172446
23	0.0	0.0	0.0	0.0	0.003206207217172446
24	0.0	0.0	0.0	0.0	0.003206207217172446
25	0.0	0.0	0.0	0.0	0.003206207217172446
26	0.0	0.0	0.0	0.0	0.003206207217172446
27	0.0	0.0	0.0	0.0	0.003206207217172446
28	0.0	0.0	0.0	0.0	0.003206207217172446
29	0.0	0.0	0.0	0.0	0.003206207217172446
30	0.0	0.0	0.0	0.0	0.003206207217172446
31	0.0	0.0	0.0	0.001603103608586223	0.003206207217172446
32	0.0	0.0	0.0	0.001603103608586223	0.003206207217172446
33	0.0	0.0	0.0	0.001603103608586223	0.003206207217172446
34	0.0	0.0	0.0	0.001603103608586223	0.003206207217172446
35	0.0	0.0	0.0	0.001603103608586223	0.003206207217172446
36	0.0	0.0	0.0	0.003206207217172446	0.003206207217172446
37	0.0	0.0	0.0	0.003206207217172446	0.003206207217172446
38	0.0	0.0	0.0	0.003206207217172446	0.003206207217172446
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	25	0.0023357742	35.2	1
GCATGGC	30	0.0057038576	29.333332	8
GATTAAG	45	0.0013834143	24.444445	1
TATCAAC	80	8.815678E-5	19.250002	1
ATCAACG	80	8.815678E-5	19.250002	2
TCAACGC	85	1.4034755E-4	18.117645	3
CAACGCA	95	3.283096E-4	16.210526	4
AACGCAG	100	4.8508577E-4	15.400001	5
ACGCAGA	115	0.0013976111	13.391303	6
CGCAGAG	115	0.0013976111	13.391303	7
AGAGTAC	125	0.0026166837	12.32	10
GCAGAGT	135	0.0046539116	11.407407	8
AGTACAT	135	0.0046539116	11.407407	12
GAGTACA	140	0.0061025824	11.0	11
CAGAGTA	140	0.0061025824	11.0	9
GTACATG	190	0.0054349257	9.263158	13
>>END_MODULE