FastQCFastQC Report
Thu 2 Feb 2017
SRR4062246_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062246_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719399
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28560.16610455164857021No Hit
GCGCAAGACGGACCAGAGCGAAAGC18910.109980289624456No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGAA901.3460522E-1015.83306613
TAGACGG400.005275910714.251425
CGTTATT1101.8735591E-1013.8143272
CGATAAC1051.366061E-913.57475910
CGAACGA1001.0088115E-813.29861316
ACGAACG1001.0088115E-813.29861315
GGTATCA7750.012.9872761
GTATCAA20650.012.9671041
GCGTTAT1251.03682396E-1012.9137631
CGCATCG3250.012.86132213
ATCGCCA3500.012.48441316
GTCCTAA3350.012.4715891
CGAGCCG2300.012.39001315
GGTTCTA3400.012.29391113
CCGATAA1251.4060788E-912.1626299
CGCCAGT3450.012.11644418
CGTCTTA550.003071066212.08964915
TTAGGAC3950.012.0233633
AGGCCCG2650.011.83309110
CGGTTCT3300.011.80351812
Produced by FastQC (version 0.11.5)