##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062246_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1719399 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.25742017995823 32.0 32.0 32.0 32.0 32.0 2 30.750104542342992 32.0 32.0 32.0 32.0 32.0 3 30.780971141660544 32.0 32.0 32.0 32.0 32.0 4 30.794652084827316 32.0 32.0 32.0 32.0 32.0 5 30.693885479751938 32.0 32.0 32.0 32.0 32.0 6 34.36992693377162 36.0 36.0 36.0 32.0 36.0 7 34.30163621125754 36.0 36.0 36.0 32.0 36.0 8 34.25308087302598 36.0 36.0 36.0 32.0 36.0 9 34.39789426421674 36.0 36.0 36.0 32.0 36.0 10 34.05165874820214 36.0 36.0 36.0 32.0 36.0 11 34.39473967357198 36.0 36.0 36.0 32.0 36.0 12 34.18695369719303 36.0 36.0 36.0 32.0 36.0 13 34.283244319672164 36.0 36.0 36.0 32.0 36.0 14 34.1820130173392 36.0 36.0 36.0 32.0 36.0 15 34.1356247153802 36.0 36.0 36.0 32.0 36.0 16 34.143006946031726 36.0 36.0 36.0 32.0 36.0 17 34.06682567571576 36.0 36.0 36.0 32.0 36.0 18 34.077068208135515 36.0 36.0 36.0 32.0 36.0 19 34.079909898749506 36.0 36.0 36.0 32.0 36.0 20 34.068661782401875 36.0 36.0 36.0 32.0 36.0 21 34.047174041627336 36.0 36.0 36.0 32.0 36.0 22 34.014861588264274 36.0 36.0 36.0 32.0 36.0 23 33.98034080513016 36.0 36.0 36.0 32.0 36.0 24 33.95751887723559 36.0 36.0 36.0 32.0 36.0 25 33.58902383914379 36.0 36.0 36.0 27.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 2.0 4 20.0 5 139.0 6 347.0 7 84.0 8 280.0 9 240.0 10 130.0 11 46.0 12 69.0 13 75.0 14 255.0 15 324.0 16 563.0 17 758.0 18 1038.0 19 1445.0 20 2235.0 21 3362.0 22 5081.0 23 7561.0 24 10585.0 25 14958.0 26 20687.0 27 26509.0 28 35063.0 29 46240.0 30 60946.0 31 82318.0 32 116549.0 33 165263.0 34 358303.0 35 757924.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.50178755983978 17.13824086896288 11.320805195817336 26.039166375380006 2 16.64601988140021 19.280956801109227 37.846022673078224 26.22700064441234 3 19.5642969392491 22.677101423651056 28.04455321737694 29.7140484197229 4 12.798891594885376 15.30338925575273 35.44430142151095 36.45341772785095 5 14.90718137157937 36.33749816736442 33.45409335154721 15.301227109508995 6 34.91745902049586 34.898312167039705 16.5059844101547 13.678244402309728 7 30.77267937095713 29.99656483438426 20.066374386473598 19.16438140818501 8 27.8606542248162 33.72528287033991 19.00344865131125 19.41061425353264 9 26.95093567465995 14.521256122195261 18.6490321821772 39.87877602096759 10 16.209358234580183 26.734926118587644 31.270543645126946 25.785172001705227 11 37.200296340129505 21.21592641818671 22.161151253446526 19.422625988237254 12 24.502533341098363 23.502999242909556 28.85277502766292 23.14169238832916 13 29.383054677172325 19.628109039873745 25.098446863152745 25.890389419801185 14 23.2165410197749 19.756151670656273 25.020860276036917 32.00644703353191 15 25.004775415694112 27.718550975815432 21.88037234265588 25.39630126583458 16 25.480560665270303 26.03089586838074 23.94578286944111 24.54276059690785 17 23.776567868165145 26.30122867291679 25.319629651196646 24.602573807721424 18 24.53872529868079 25.486515331572214 26.18143326022187 23.793326109525122 19 25.487781804421545 25.016635375278344 25.280530934812035 24.215051885488073 20 25.65105773596768 24.785018220768183 24.97747144636807 24.586452596896066 21 26.078290406238086 24.59211740359231 24.576225306854266 24.753366883315337 22 25.67112667774392 24.766975968104962 25.053707600105728 24.50818975404539 23 24.241727513178237 24.84947473317563 25.508619405070128 25.40017834857601 24 24.59059719747329 25.06265255642805 25.571149727530507 24.77560051856815 25 24.58494310194383 24.78920447500636 25.59390929723959 25.03194312581022 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 265.0 1 265.0 2 417.5 3 570.0 4 570.0 5 570.0 6 1181.0 7 1792.0 8 1792.0 9 1792.0 10 2140.0 11 2488.0 12 2488.0 13 2488.0 14 3637.5 15 4787.0 16 4787.0 17 4787.0 18 7912.5 19 11038.0 20 11038.0 21 11038.0 22 17534.5 23 24031.0 24 24031.0 25 24031.0 26 35807.0 27 47583.0 28 47583.0 29 47583.0 30 61451.0 31 75319.0 32 75319.0 33 75319.0 34 91652.5 35 107986.0 36 107986.0 37 107986.0 38 125790.0 39 143594.0 40 143594.0 41 143594.0 42 163286.5 43 182979.0 44 182979.0 45 182979.0 46 201699.5 47 220420.0 48 220420.0 49 220420.0 50 226302.0 51 232184.0 52 232184.0 53 232184.0 54 217064.5 55 201945.0 56 201945.0 57 201945.0 58 185800.0 59 169655.0 60 169655.0 61 169655.0 62 149086.0 63 128517.0 64 128517.0 65 128517.0 66 104774.0 67 81031.0 68 81031.0 69 81031.0 70 61206.5 71 41382.0 72 41382.0 73 41382.0 74 31154.0 75 20926.0 76 20926.0 77 20926.0 78 16584.5 79 12243.0 80 12243.0 81 12243.0 82 8499.0 83 4755.0 84 4755.0 85 4755.0 86 3490.5 87 2226.0 88 2226.0 89 2226.0 90 1504.5 91 783.0 92 783.0 93 783.0 94 483.5 95 184.0 96 184.0 97 184.0 98 450.0 99 716.0 100 716.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 9.3055771231692E-4 2 1.744795710594225E-4 3 9.887175693367276E-4 4 0.01000349540740689 5 0.03274399950215162 6 0.06403400257880806 7 0.10870077277002023 8 0.11143428604995116 9 0.12108882231523922 10 0.13510534785701284 11 0.1426661292695878 12 0.13370951128853745 13 0.12963832129715092 14 0.11748291118001115 15 0.13208103529198284 16 0.12108882231523922 17 0.12207753988457595 18 0.11224852404822848 19 0.09735960065115776 20 0.09183441423427605 21 0.09096201637897894 22 0.10265214763996024 23 0.08229619768302762 24 0.09259049237553355 25 0.08735610524375087 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1719399.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.798899415269574 #Duplication Level Percentage of deduplicated Percentage of total 1 76.78540889011762 40.541850805496665 2 13.701959972499832 14.46896812760137 3 4.1160994814586385 6.519765675143336 4 1.7397380533615625 3.6742501795341607 5 0.8856589277543601 2.338090832136898 6 0.5597832835616687 1.7733564769873125 7 0.3723263930298337 1.3760896642662621 8 0.27600481009727384 1.1658200165165231 9 0.1884812059856852 0.8956440212856209 >10 1.1932836147285233 11.645926413286233 >50 0.09880410304439538 3.627112845288837 >100 0.07283603838333734 7.6717895495545125 >500 0.00740482920086346 2.6914273432662092 >1k 0.002210396776377152 1.6099080496362217 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2856 0.16610455164857021 No Hit GCGCAAGACGGACCAGAGCGAAAGC 1891 0.109980289624456 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.744795710594225E-4 7 0.0 0.0 0.0 5.81598570198075E-5 1.744795710594225E-4 8 0.0 0.0 0.0 1.16319714039615E-4 1.744795710594225E-4 9 0.0 0.0 0.0 1.744795710594225E-4 1.744795710594225E-4 10 0.0 0.0 0.0 2.3263942807923E-4 1.744795710594225E-4 11 0.0 0.0 0.0 2.9079928509903754E-4 1.744795710594225E-4 12 0.0 0.0 0.0 2.9079928509903754E-4 4.071189991386525E-4 13 0.0 0.0 0.0 3.48959142118845E-4 4.6527885615846E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAACGAA 90 1.3460522E-10 15.833066 13 TAGACGG 40 0.0052759107 14.25142 5 CGTTATT 110 1.8735591E-10 13.814327 2 CGATAAC 105 1.366061E-9 13.574759 10 CGAACGA 100 1.0088115E-8 13.298613 16 ACGAACG 100 1.0088115E-8 13.298613 15 GGTATCA 775 0.0 12.987276 1 GTATCAA 2065 0.0 12.967104 1 GCGTTAT 125 1.03682396E-10 12.913763 1 CGCATCG 325 0.0 12.861322 13 ATCGCCA 350 0.0 12.484413 16 GTCCTAA 335 0.0 12.471589 1 CGAGCCG 230 0.0 12.390013 15 GGTTCTA 340 0.0 12.293911 13 CCGATAA 125 1.4060788E-9 12.162629 9 CGCCAGT 345 0.0 12.116444 18 CGTCTTA 55 0.0030710662 12.089649 15 TTAGGAC 395 0.0 12.023363 3 AGGCCCG 265 0.0 11.833091 10 CGGTTCT 330 0.0 11.803518 12 >>END_MODULE