FastQCFastQC Report
Thu 2 Feb 2017
SRR4062246_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062246_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1719399
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT19570.11381884018776328No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA19450.025.234361
GGTATCA9300.023.1926361
TAGGACG4550.019.8227864
CTAGCGG3500.018.22782329
GGCGTTA5200.017.77160342
AATACCG750.001291777717.5987685
GTCCTAC7400.017.5479871
TCAACGC27750.017.5194934
ATACCGT4400.017.4987756
CGCAATA3650.017.47975336
CGGATCG1408.382085E-817.28500626
ATCAACG28150.017.1142543
AACGCAG28150.017.1142546
CGTTATT5300.017.02213144
GTATAGA2201.8189894E-1217.0072171
CAACGCA28650.016.9691435
ACCGTCG4450.016.8078128
TAGCGGC3950.016.70817630
TACCGTC4900.016.611087
GTAGGAC7650.016.3910083
Produced by FastQC (version 0.11.5)