##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062246_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1719399
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.283122183972424	32.0	32.0	32.0	32.0	32.0
2	31.4577646026315	32.0	32.0	32.0	32.0	32.0
3	31.583036863462176	32.0	32.0	32.0	32.0	32.0
4	31.659712492562807	32.0	32.0	32.0	32.0	32.0
5	31.637405861001433	32.0	32.0	32.0	32.0	32.0
6	35.28677462299326	36.0	36.0	36.0	36.0	36.0
7	35.28710846057256	36.0	36.0	36.0	36.0	36.0
8	35.23871538834209	36.0	36.0	36.0	36.0	36.0
9	35.32366309390665	36.0	36.0	36.0	36.0	36.0
10	35.20991869833587	36.0	36.0	36.0	36.0	36.0
11	35.32148558885983	36.0	36.0	36.0	36.0	36.0
12	35.24769527026595	36.0	36.0	36.0	36.0	36.0
13	35.28279299918169	36.0	36.0	36.0	36.0	36.0
14	35.24711599809003	36.0	36.0	36.0	36.0	36.0
15	35.2250757386738	36.0	36.0	36.0	36.0	36.0
16	35.2314320294475	36.0	36.0	36.0	36.0	36.0
17	35.218047701551534	36.0	36.0	36.0	36.0	36.0
18	35.215763763966365	36.0	36.0	36.0	36.0	36.0
19	35.20194265554417	36.0	36.0	36.0	36.0	36.0
20	35.20589345463153	36.0	36.0	36.0	36.0	36.0
21	35.193453642813566	36.0	36.0	36.0	36.0	36.0
22	35.1725655301649	36.0	36.0	36.0	36.0	36.0
23	35.14510768006728	36.0	36.0	36.0	36.0	36.0
24	35.12911953537253	36.0	36.0	36.0	36.0	36.0
25	35.11377929148499	36.0	36.0	36.0	36.0	36.0
26	35.07254976884365	36.0	36.0	36.0	36.0	36.0
27	35.0599651389817	36.0	36.0	36.0	36.0	36.0
28	35.038601860301185	36.0	36.0	36.0	36.0	36.0
29	35.01286496037278	36.0	36.0	36.0	36.0	36.0
30	34.991663947693354	36.0	36.0	36.0	36.0	36.0
31	34.99247527769878	36.0	36.0	36.0	36.0	36.0
32	34.96991274276651	36.0	36.0	36.0	36.0	36.0
33	34.950330900506515	36.0	36.0	36.0	36.0	36.0
34	34.94811733634834	36.0	36.0	36.0	36.0	36.0
35	34.92585723267258	36.0	36.0	36.0	36.0	36.0
36	34.90211696063567	36.0	36.0	36.0	36.0	36.0
37	34.88127479427405	36.0	36.0	36.0	32.0	36.0
38	34.86832608370715	36.0	36.0	36.0	32.0	36.0
39	34.82763570294039	36.0	36.0	36.0	32.0	36.0
40	34.830539043002815	36.0	36.0	36.0	32.0	36.0
41	34.803752357655206	36.0	36.0	36.0	32.0	36.0
42	34.77560298685762	36.0	36.0	36.0	32.0	36.0
43	34.77230474136602	36.0	36.0	36.0	32.0	36.0
44	34.728941915169194	36.0	36.0	36.0	32.0	36.0
45	34.71182663244541	36.0	36.0	36.0	32.0	36.0
46	34.67798631963843	36.0	36.0	36.0	32.0	36.0
47	34.65945717079049	36.0	36.0	36.0	32.0	36.0
48	34.635519155239706	36.0	36.0	36.0	32.0	36.0
49	34.61224939644608	36.0	36.0	36.0	32.0	36.0
50	34.21623369561108	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	17.0
21	49.0
22	118.0
23	323.0
24	822.0
25	1895.0
26	4133.0
27	7693.0
28	13359.0
29	21154.0
30	31788.0
31	46806.0
32	70219.0
33	119062.0
34	272029.0
35	1129928.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.201388404056765	17.65840047288586	11.71375744120344	26.426453681853936
2	16.140353326144435	19.797791915166364	37.48887640331806	26.57297835537114
3	18.9965045742967	23.098308120903344	28.384194394523636	29.520992910276316
4	12.566891105554905	15.754923668095655	35.47117335766742	36.207011868682024
5	14.554795018491925	36.72265716101963	33.28581673014815	15.436731090340288
6	35.02334540734072	35.05166930712878	16.385928547001388	13.539056738529112
7	30.79360869699238	30.048580928568647	20.01961150378708	19.138198870651895
8	27.971634274534303	33.682176155738134	18.966394653015385	19.379794916712175
9	27.021476690401702	14.271149395806326	18.56642931629017	40.14094459750181
10	16.23721353312442	26.73938987877138	31.30413238137113	25.71926420673306
11	37.470825561722435	21.084053206963596	22.17705139993684	19.268069831377126
12	24.536887598515527	23.492278406582763	28.869564307063104	23.1012696878386
13	29.547650080057043	19.431440869745767	25.18065905586778	25.84024999432941
14	23.32832576964393	19.61778505163723	25.068410531819545	31.985478646899296
15	25.029617907187333	27.670075415886597	21.872177429439006	25.428129247487057
16	25.686481720477637	25.93372157337836	23.86425828334932	24.515538422794688
17	23.988614626389804	26.235213583350927	25.20688915138371	24.56928263887556
18	24.585406253598652	25.324213065766195	26.261927167362455	23.8284535132727
19	25.597199951843642	24.899514307034025	25.239749470599904	24.263536270522433
20	25.770940226614726	24.55679152073347	25.113266650924686	24.55900160172712
21	26.1231547320632	24.425002239155795	24.679626241277468	24.772216787503535
22	25.723116042291522	24.67449382022439	25.122789998133065	24.479600139351017
23	24.373254111761273	24.635729851802697	25.61479402371878	25.376222012717246
24	24.666711644067462	24.90057474138964	25.69469728076716	24.73801633377574
25	24.79776340193684	24.48649148509623	25.672546828917348	25.043198284049584
26	24.338774817027495	25.472145838276965	25.89590225336516	24.293177091330385
27	24.858772505814567	25.050119548281398	25.331207772063213	24.759900173840823
28	24.52735485056701	24.86823631501379	25.633692884470204	24.970715949948993
29	24.414342054832204	24.96093093426684	25.569692990134296	25.055034020766655
30	24.45199500050309	25.046397727543702	25.849597498623044	24.65200977333016
31	25.105139093015016	24.717326910896837	25.047793140470247	25.1297408556179
32	24.885365655061708	25.026230385371473	25.056008561225557	25.03239539834126
33	24.41554785772612	24.86122969335601	25.601260448485156	25.121962000432713
34	25.01975997231568	24.957586578922115	25.725361102952508	24.297292345809694
35	25.41971182870244	24.762270893116995	25.668345940488337	24.149671337692233
36	24.29130677789946	25.39954414251043	25.416643160363776	24.89250591922633
37	25.28982915303526	25.02903671392221	25.175834242093785	24.505299890948745
38	24.685602352915176	24.972272288165804	25.40719169895993	24.934933659959093
39	25.33666087777846	24.758489367716148	24.957455742922182	24.947394011583203
40	25.614212424472065	24.819398893914553	25.329170633658194	24.237218047955185
41	24.564884990051606	25.11985473774451	25.881411421489116	24.433848850714767
42	25.49279857710609	25.4875057653842	25.20937141753712	23.810324239972594
43	24.88732522978441	24.66764650628239	25.6079551964749	24.83707306745829
44	24.46884555007471	25.335699383652035	25.232751766540396	24.962703299732862
45	24.625357833723204	25.422682906685807	25.257506511090668	24.694452748500325
46	24.656762217613277	25.04818647847283	25.196148338744255	25.09890296516964
47	24.970658130286044	25.0120682615771	25.501836120568623	24.515437487568235
48	25.335159626133436	25.455322712917237	24.574921626447512	24.63459603450181
49	24.788216798530126	25.455860978323518	24.82456818910168	24.931354034044677
50	24.930526879031273	25.740067582893733	24.68718193291729	24.642223605157703
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	119.0
1	89.5
2	60.0
3	150.5
4	241.0
5	246.5
6	252.0
7	223.0
8	194.0
9	282.5
10	371.0
11	572.0
12	773.0
13	1444.5
14	2116.0
15	2680.5
16	3245.0
17	3254.0
18	3263.0
19	3208.0
20	3153.0
21	3877.0
22	4601.0
23	5509.0
24	6417.0
25	8376.0
26	10335.0
27	13582.5
28	16830.0
29	20089.0
30	23348.0
31	26720.0
32	30092.0
33	35139.0
34	40186.0
35	46725.5
36	53265.0
37	60591.0
38	67917.0
39	73073.5
40	78230.0
41	83340.0
42	88450.0
43	91621.5
44	94793.0
45	101989.5
46	109186.0
47	115903.5
48	122621.0
49	126935.0
50	131249.0
51	129216.0
52	127183.0
53	125277.0
54	123371.0
55	124187.0
56	125003.0
57	120326.5
58	115650.0
59	106313.0
60	96976.0
61	85155.5
62	73335.0
63	63248.5
64	53162.0
65	44351.5
66	35541.0
67	30732.0
68	25923.0
69	23360.5
70	20798.0
71	16267.0
72	11736.0
73	10061.0
74	8386.0
75	6073.5
76	3761.0
77	3289.5
78	2818.0
79	2387.0
80	1956.0
81	1528.0
82	1100.0
83	906.0
84	712.0
85	568.5
86	425.0
87	285.5
88	146.0
89	98.5
90	51.0
91	34.0
92	17.0
93	19.0
94	21.0
95	14.5
96	8.0
97	9.5
98	11.0
99	6.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034372475498706236
2	0.004710948418604408
3	5.234387131782675E-4
4	0.0
5	0.0
6	1.744795710594225E-4
7	0.0
8	0.0
9	0.0
10	1.744795710594225E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	9.3055771231692E-4
17	0.0
18	2.9079928509903754E-4
19	0.0
20	2.9079928509903754E-4
21	5.81598570198075E-5
22	0.0
23	1.16319714039615E-4
24	9.887175693367276E-4
25	1.16319714039615E-4
26	8.723978552971126E-4
27	5.815985701980751E-4
28	0.0019192752816536477
29	4.6527885615846E-4
30	0.001512156282514995
31	5.815985701980751E-4
32	0.0011050372833763425
33	6.397584272178825E-4
34	8.142379982773051E-4
35	2.9079928509903754E-4
36	1.16319714039615E-4
37	0.00139583656847538
38	0.0
39	7.560781412574975E-4
40	1.16319714039615E-4
41	0.001512156282514995
42	0.005001747703703445
43	0.004071189991386525
44	0.004536468847544985
45	0.0014539964254951874
46	0.0021519147097328775
47	7.560781412574975E-4
48	0.004013030134366718
49	0.004071189991386525
50	0.0016866358535744174
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1719399.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.662681213422715
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.75111430501278	41.954216940298814
2	13.927101158967254	15.22585381759434
3	4.157843838504217	6.818366768380496
4	1.6781423692476773	3.669270454436948
5	0.8852040136889844	2.419381240456161
6	0.5124977860932073	1.6808701862278737
7	0.35065404133893824	1.3417383054536112
8	0.25798453253592335	1.1281701008004048
9	0.19511744303307635	0.9599078328925698
>10	1.1250729017508245	11.265500515976743
>50	0.08946295249442929	3.357593283562441
>100	0.0623107075498859	6.802473078577602
>500	0.0056739905498405345	2.0792124936986203
>1k	0.0018199592329677186	1.2974449816434594
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1957	0.11381884018776328	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.3263942807923E-4
2	0.0	0.0	0.0	0.0	2.3263942807923E-4
3	0.0	5.81598570198075E-5	0.0	0.0	2.3263942807923E-4
4	0.0	5.81598570198075E-5	0.0	0.0	2.3263942807923E-4
5	0.0	5.81598570198075E-5	0.0	0.0	2.3263942807923E-4
6	0.0	5.81598570198075E-5	0.0	0.0	3.48959142118845E-4
7	0.0	5.81598570198075E-5	0.0	0.0	3.48959142118845E-4
8	0.0	5.81598570198075E-5	0.0	0.0	3.48959142118845E-4
9	0.0	5.81598570198075E-5	0.0	0.0	3.48959142118845E-4
10	0.0	5.81598570198075E-5	0.0	0.0	3.48959142118845E-4
11	0.0	5.81598570198075E-5	0.0	0.0	4.071189991386525E-4
12	0.0	5.81598570198075E-5	0.0	0.0	7.560781412574975E-4
13	0.0	5.81598570198075E-5	0.0	0.0	9.3055771231692E-4
14	0.0	5.81598570198075E-5	0.0	5.81598570198075E-5	9.3055771231692E-4
15	0.0	5.81598570198075E-5	0.0	5.81598570198075E-5	9.887175693367276E-4
16	0.0	5.81598570198075E-5	0.0	5.81598570198075E-5	0.0011050372833763425
17	0.0	5.81598570198075E-5	0.0	5.81598570198075E-5	0.0011050372833763425
18	0.0	5.81598570198075E-5	0.0	5.81598570198075E-5	0.0011631971403961501
19	0.0	5.81598570198075E-5	0.0	1.16319714039615E-4	0.0011631971403961501
20	0.0	5.81598570198075E-5	0.0	4.6527885615846E-4	0.001279516854435765
21	0.0	5.81598570198075E-5	0.0	7.560781412574975E-4	0.00139583656847538
22	0.0	5.81598570198075E-5	0.0	0.001046877426356535	0.001512156282514995
23	0.0	5.81598570198075E-5	0.0	0.001279516854435765	0.0015703161395348025
24	0.0	5.81598570198075E-5	0.0	0.00209375485271307	0.00162847599655461
25	0.0	5.81598570198075E-5	0.0	0.002442713994831915	0.00162847599655461
26	0.0	5.81598570198075E-5	0.0	0.002849832993970568	0.0017447957105942251
27	0.0	5.81598570198075E-5	0.0	0.00372223084926768	0.00186111542463384
28	0.0	5.81598570198075E-5	0.0	0.004071189991386525	0.00186111542463384
29	0.0	5.81598570198075E-5	0.0	0.005932305416020365	0.00186111542463384
30	0.0	5.81598570198075E-5	0.0	0.008491339124891896	0.00186111542463384
31	0.0	5.81598570198075E-5	0.0	0.013493086828595341	0.00186111542463384
32	0.0	5.81598570198075E-5	0.0	0.01890195353143744	0.00186111542463384
33	0.0	5.81598570198075E-5	0.0	0.02308946323686358	0.00209375485271307
34	0.0	5.81598570198075E-5	0.0	0.028963608795864137	0.002210074566752685
35	0.0	5.81598570198075E-5	0.0	0.03594279163824104	0.002210074566752685
36	0.0	5.81598570198075E-5	0.0	0.04513204904737062	0.002210074566752685
37	0.0	5.81598570198075E-5	0.0	0.06013729215848096	0.002210074566752685
38	0.0	5.81598570198075E-5	0.0	0.07758524926442321	0.0022682344237724924
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1945	0.0	25.23436	1
GGTATCA	930	0.0	23.192636	1
TAGGACG	455	0.0	19.822786	4
CTAGCGG	350	0.0	18.227823	29
GGCGTTA	520	0.0	17.771603	42
AATACCG	75	0.0012917777	17.598768	5
GTCCTAC	740	0.0	17.547987	1
TCAACGC	2775	0.0	17.519493	4
ATACCGT	440	0.0	17.498775	6
CGCAATA	365	0.0	17.479753	36
CGGATCG	140	8.382085E-8	17.285006	26
ATCAACG	2815	0.0	17.114254	3
AACGCAG	2815	0.0	17.114254	6
CGTTATT	530	0.0	17.022131	44
GTATAGA	220	1.8189894E-12	17.007217	1
CAACGCA	2865	0.0	16.969143	5
ACCGTCG	445	0.0	16.807812	8
TAGCGGC	395	0.0	16.708176	30
TACCGTC	490	0.0	16.61108	7
GTAGGAC	765	0.0	16.391008	3
>>END_MODULE