FastQCFastQC Report
Thu 2 Feb 2017
SRR4062245_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062245_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566218
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47040.1319044433066066No Hit
GCGCAAGACGGACCAGAGCGAAAGC35830.10047058256113339No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13600.013.2676061
TAGATCG655.4505224E-513.1553445
CGGTCCA4500.012.66793110
GTATCAA35950.012.6271951
CAAGACG7200.012.5350924
ATTTGCG2050.012.04879916
AAGACGG7600.012.0013675
TCGCGTA2950.011.9164449
TTTGCGC2500.011.39921817
CGCGTAA3100.011.33984110
CGGACCA7550.011.3256349
GACGGAC7450.011.2229127
AACCGCG1451.2169039E-911.1393557
TTACCGC4600.010.94566916
CGCGCTA700.001492203210.85715916
GCGCAAG8350.010.5772891
GCGTAAC3150.010.55660911
CGCAAGA8100.010.554092
GTCTTAG3250.010.51954651
GAGCGAA7900.010.46204116
Produced by FastQC (version 0.11.5)