FastQCFastQC Report
Thu 2 Feb 2017
SRR4062245_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062245_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566218
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36150.028.0017951
CGCAATA5900.025.35616936
GGTATCA16700.023.4552381
TCTAGCG6950.022.15754528
CTAGCGG7200.021.38818629
TAGCGGC7450.021.26104730
GCGCAAT7550.020.39696935
CGAATGC7850.019.90022743
CAATACG7650.019.5565638
TATAGCG808.99807E-519.2490985
ATACGAA7800.019.1810140
TCAACGC51500.019.0515354
TACGAAT7900.018.93821141
AATACGA8100.018.74222839
ATCAACG52300.018.7180543
CAACGCA52450.018.7064655
GTATTAC4500.018.0936241
AACGCAG55150.017.9103136
TATCAAC55450.017.735312
GCAATAC8800.017.2501637
Produced by FastQC (version 0.11.5)