##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062245_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3566218
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.005002218036026	32.0	32.0	32.0	32.0	32.0
2	30.85019087447823	32.0	32.0	32.0	32.0	32.0
3	31.261694321547363	32.0	32.0	32.0	32.0	32.0
4	31.44673629037821	32.0	32.0	32.0	32.0	32.0
5	31.33624472760779	32.0	32.0	32.0	32.0	32.0
6	34.99236137555248	36.0	36.0	36.0	36.0	36.0
7	35.01601808975222	36.0	36.0	36.0	36.0	36.0
8	34.922154226129756	36.0	36.0	36.0	36.0	36.0
9	35.107443796200904	36.0	36.0	36.0	36.0	36.0
10	34.87076084524278	36.0	36.0	36.0	32.0	36.0
11	35.123532268638655	36.0	36.0	36.0	36.0	36.0
12	34.98972048259529	36.0	36.0	36.0	36.0	36.0
13	35.07078591381682	36.0	36.0	36.0	36.0	36.0
14	35.00273847532596	36.0	36.0	36.0	32.0	36.0
15	34.9517314981866	36.0	36.0	36.0	32.0	36.0
16	34.98175377949413	36.0	36.0	36.0	36.0	36.0
17	34.95218716298331	36.0	36.0	36.0	32.0	36.0
18	34.9600913348539	36.0	36.0	36.0	32.0	36.0
19	34.950412173344425	36.0	36.0	36.0	32.0	36.0
20	34.959189258760965	36.0	36.0	36.0	32.0	36.0
21	34.95824456048396	36.0	36.0	36.0	32.0	36.0
22	34.93451045337105	36.0	36.0	36.0	32.0	36.0
23	34.89407181501524	36.0	36.0	36.0	32.0	36.0
24	34.87914816200244	36.0	36.0	36.0	32.0	36.0
25	34.855626324582516	36.0	36.0	36.0	32.0	36.0
26	34.80767861078599	36.0	36.0	36.0	32.0	36.0
27	34.78869771842327	36.0	36.0	36.0	32.0	36.0
28	34.76940248745309	36.0	36.0	36.0	32.0	36.0
29	34.7440961264847	36.0	36.0	36.0	32.0	36.0
30	34.722659411174526	36.0	36.0	36.0	32.0	36.0
31	34.727800151308756	36.0	36.0	36.0	32.0	36.0
32	34.6959667075877	36.0	36.0	36.0	32.0	36.0
33	34.67088691717668	36.0	36.0	36.0	32.0	36.0
34	34.65504099861534	36.0	36.0	36.0	32.0	36.0
35	34.6451262934571	36.0	36.0	36.0	32.0	36.0
36	34.604887025975415	36.0	36.0	36.0	32.0	36.0
37	34.60813500464638	36.0	36.0	36.0	32.0	36.0
38	34.586284405496244	36.0	36.0	36.0	32.0	36.0
39	34.57276139596626	36.0	36.0	36.0	32.0	36.0
40	34.55724495810408	36.0	36.0	36.0	32.0	36.0
41	34.52012888724133	36.0	36.0	36.0	32.0	36.0
42	34.489288932981665	36.0	36.0	36.0	32.0	36.0
43	34.49019185030192	36.0	36.0	36.0	32.0	36.0
44	34.43141669970821	36.0	36.0	36.0	32.0	36.0
45	34.42724252976122	36.0	36.0	36.0	32.0	36.0
46	34.406765374410654	36.0	36.0	36.0	32.0	36.0
47	34.39017777376481	36.0	36.0	36.0	32.0	36.0
48	34.358625580376746	36.0	36.0	36.0	32.0	36.0
49	34.33991107666441	36.0	36.0	36.0	32.0	36.0
50	33.81588085753591	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	21.0
21	55.0
22	142.0
23	386.0
24	1166.0
25	2961.0
26	6700.0
27	13594.0
28	26032.0
29	45592.0
30	76258.0
31	126395.0
32	213385.0
33	399844.0
34	874809.0
35	1778875.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.86823095225607	17.564681256032117	12.265113843442233	25.30197394826958
2	16.36655731250678	19.291582459102955	37.68896401632153	26.65289621206874
3	18.97859689777276	22.79286817497109	28.36987817886317	29.858656748392974
4	13.037628679810757	15.901981259688139	35.199298079533044	35.86109198096806
5	15.24497380698544	36.05665722061859	32.91414041429885	15.784228558097121
6	35.62824205957412	34.784994614735574	16.02304741752666	13.563715908163646
7	31.46644428355193	30.031142235275578	19.56470412072397	18.937709360448522
8	28.292185166470475	33.54410751109438	18.44626436185337	19.71744296058177
9	26.722679320221033	14.800160842663013	18.383929417663193	40.093230419452766
10	16.07370839952162	26.832678343846343	31.101209545694807	25.99240371093723
11	37.41860424685199	21.4379210693233	21.587154795360238	19.556319888464476
12	24.476714547456158	23.46185791221961	28.43611916041027	23.62530837991396
13	29.21860076977908	19.520848136597373	25.063190191962466	26.19736090166109
14	23.501816209777417	19.387765975046957	24.937342585338303	32.17307522983732
15	25.11691657660861	27.149209610853852	21.959510046777847	25.774363765759695
16	25.925130390892264	26.133110874320003	23.245779821658907	24.695978913128823
17	24.13545666585722	26.37483182463887	24.86620279523013	24.623508714273775
18	24.883581051619153	25.624142122670673	25.473029528505712	24.01924729720446
19	25.451444639671493	25.390708027383628	25.180513361774292	23.977333971170577
20	25.631987814507735	24.822668784322172	24.83256725350849	24.712776147661604
21	26.2600884185992	24.62499488253382	24.649362433816442	24.46555426505054
22	25.767746110865907	24.503269289762994	25.045496377394766	24.683488221976337
23	24.56201499740061	24.718146787436996	25.34522567044415	25.374612544718246
24	24.642138487526164	25.213477487420725	25.17124747348987	24.97313655156324
25	24.8725747705757	24.788984605220836	25.2114219890085	25.127018635194965
26	24.698594332858413	25.446475765428083	25.404862925792028	24.450066975921477
27	25.14003701418877	25.18751051539454	24.912567999551342	24.759884470865348
28	24.844587779407604	24.83267020193424	25.381467634243098	24.94127438441506
29	24.63503879899086	25.292068506514354	25.356787236613975	24.71610545788081
30	24.664662845778977	25.275875886486045	25.434112120312257	24.625349147422725
31	25.036502429335865	25.05102769025077	24.819829280514476	25.092640599898886
32	25.066948124527155	25.0532640680793	24.710209259507476	25.16957854788607
33	24.631541815237007	24.974538810367978	25.16395547993221	25.22996389446281
34	25.21109304021087	24.945852728394367	25.2856541977455	24.557400033649262
35	25.565522866974018	24.7599624587763	25.219553627335216	24.454961046914473
36	24.58814866410148	25.210096758074528	25.07822020776095	25.123534370063044
37	25.505598443147694	24.92304068510475	24.96269106632606	24.608669805421503
38	24.83050671607849	24.85616414924719	25.27767511688854	25.03565401778579
39	25.351439294350946	24.95987316442861	24.718270917550146	24.970416623670292
40	25.42157441433004	25.120947559246986	24.991146075040344	24.466331951382628
41	24.626484604696618	25.376698068103586	25.30976379520412	24.687053531995677
42	25.48310198148157	25.21224350958997	24.975820714630032	24.328833794298426
43	25.26113356180037	24.562777071899816	25.08923646562745	25.086852900672362
44	24.724242566151638	25.174652216841597	25.012065044963848	25.089040172042914
45	24.952630975318193	25.276341510127594	24.940993772273675	24.830033742280538
46	24.772836379380355	25.12369074565526	25.02285376506666	25.08061910989773
47	24.907302850134165	25.080569337019444	25.243376461611756	24.768751351234638
48	25.435610027898896	25.486001338720744	24.47290324335526	24.605485390025102
49	24.914598533186695	25.453506886238124	24.720464776907423	24.91142980366776
50	25.117275775363296	25.33389490733328	24.700806215749292	24.848023101554134
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	12.0
1	14.5
2	17.0
3	78.5
4	140.0
5	167.5
6	195.0
7	186.5
8	178.0
9	221.5
10	265.0
11	439.0
12	613.0
13	1125.5
14	1638.0
15	2287.0
16	2936.0
17	3426.5
18	3917.0
19	4583.5
20	5250.0
21	7120.5
22	8991.0
23	11688.0
24	14385.0
25	18833.0
26	23281.0
27	28498.5
28	33716.0
29	39952.5
30	46189.0
31	53134.0
32	60079.0
33	68812.5
34	77546.0
35	88524.0
36	99502.0
37	114728.5
38	129955.0
39	143885.5
40	157816.0
41	170967.5
42	184119.0
43	193048.5
44	201978.0
45	218506.0
46	235034.0
47	248197.5
48	261361.0
49	269671.0
50	277981.0
51	272984.5
52	267988.0
53	266336.0
54	264684.0
55	264316.5
56	263949.0
57	254262.0
58	244575.0
59	224095.5
60	203616.0
61	178050.0
62	152484.0
63	132014.0
64	111544.0
65	94053.5
66	76563.0
67	64888.0
68	53213.0
69	46352.0
70	39491.0
71	30797.0
72	22103.0
73	19138.5
74	16174.0
75	12147.0
76	8120.0
77	6943.0
78	5766.0
79	4856.5
80	3947.0
81	3100.0
82	2253.0
83	1774.5
84	1296.0
85	1018.5
86	741.0
87	517.5
88	294.0
89	221.0
90	148.0
91	101.5
92	55.0
93	49.0
94	43.0
95	40.0
96	37.0
97	30.0
98	23.0
99	20.0
100	17.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.025993924095498368
2	0.0036172774631276047
3	1.6824546340128392E-4
4	5.6081821133761315E-5
5	0.0
6	1.4020455283440328E-4
7	0.0
8	0.0
9	0.0
10	8.412273170064196E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.047363902038517E-4
17	0.0
18	8.412273170064196E-5
19	0.0
20	2.8040910566880657E-4
21	0.0
22	0.0
23	0.0
24	9.533909592739423E-4
25	2.523681951019259E-4
26	3.084500162356872E-4
27	5.047363902038517E-4
28	0.0015983319023121975
29	4.7669547963697116E-4
30	8.692682275733003E-4
31	4.7669547963697116E-4
32	6.729818536051357E-4
33	1.6824546340128392E-4
34	5.047363902038517E-4
35	1.4020455283440328E-4
36	1.1216364226752263E-4
37	0.0013459637072102714
38	0.0
39	7.290636747388971E-4
40	8.412273170064196E-5
41	9.253500487070616E-4
42	0.004178095674465218
43	0.003673359284261366
44	0.003673359284261366
45	0.0019348228291147652
46	0.0018507000974141233
47	9.81431869840823E-4
48	0.0035611956419938436
49	0.003364909268025679
50	0.0014300864389109136
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	3566218.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.13890969298338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.6977247755709	34.51450298163585
2	14.975275903486374	14.417869086908814
3	5.514054732825057	7.963217484768991
4	2.600084896283088	5.006610080650466
5	1.3859144462798698	3.3358205185833856
6	0.8429078224962129	2.4345998127992674
7	0.5638113486764242	1.8998884518469488
8	0.4158076952917626	1.601322327463817
9	0.28710367555008903	1.2438772118846404
>10	1.5388334885470414	13.319523049085625
>50	0.10170741613369362	3.3846362762926976
>100	0.06683185768446091	6.19522915753546
>500	0.0070178408370502095	2.349418969422458
>1k	0.00292410033804933	2.333484591121601
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.412273170064196E-5
2	0.0	0.0	0.0	0.0	8.412273170064196E-5
3	0.0	0.0	0.0	0.0	8.412273170064196E-5
4	0.0	0.0	0.0	0.0	8.412273170064196E-5
5	0.0	0.0	0.0	0.0	1.1216364226752263E-4
6	0.0	0.0	0.0	0.0	1.1216364226752263E-4
7	2.8040910566880658E-5	0.0	0.0	0.0	1.1216364226752263E-4
8	2.8040910566880658E-5	0.0	0.0	0.0	1.4020455283440328E-4
9	2.8040910566880658E-5	0.0	0.0	8.412273170064196E-5	1.4020455283440328E-4
10	2.8040910566880658E-5	0.0	0.0	8.412273170064196E-5	1.6824546340128392E-4
11	2.8040910566880658E-5	0.0	0.0	8.412273170064196E-5	1.6824546340128392E-4
12	2.8040910566880658E-5	0.0	0.0	8.412273170064196E-5	5.608182113376131E-4
13	2.8040910566880658E-5	0.0	0.0	8.412273170064196E-5	7.851454958726584E-4
14	2.8040910566880658E-5	0.0	0.0	8.412273170064196E-5	7.851454958726584E-4
15	2.8040910566880658E-5	0.0	0.0	1.1216364226752263E-4	0.0010375136909745843
16	2.8040910566880658E-5	0.0	0.0	1.1216364226752263E-4	0.0010375136909745843
17	2.8040910566880658E-5	0.0	0.0	1.1216364226752263E-4	0.0010375136909745843
18	2.8040910566880658E-5	0.0	0.0	1.1216364226752263E-4	0.0011496773332421069
19	2.8040910566880658E-5	0.0	0.0	1.4020455283440328E-4	0.0011777182438089877
20	2.8040910566880658E-5	0.0	0.0	1.4020455283440328E-4	0.0012898818860765102
21	2.8040910566880658E-5	0.0	0.0	1.6824546340128392E-4	0.0013179227966433908
22	2.8040910566880658E-5	2.8040910566880658E-5	0.0	1.6824546340128392E-4	0.0013179227966433908
23	2.8040910566880658E-5	2.8040910566880658E-5	0.0	1.6824546340128392E-4	0.0013459637072102714
24	2.8040910566880658E-5	2.8040910566880658E-5	0.0	1.6824546340128392E-4	0.0013459637072102714
25	2.8040910566880658E-5	2.8040910566880658E-5	0.0	1.6824546340128392E-4	0.0013459637072102714
26	5.6081821133761315E-5	2.8040910566880658E-5	0.0	1.6824546340128392E-4	0.0013740046177771522
27	5.6081821133761315E-5	2.8040910566880658E-5	0.0	1.6824546340128392E-4	0.0014020455283440328
28	5.6081821133761315E-5	2.8040910566880658E-5	0.0	1.962863739681646E-4	0.0014020455283440328
29	5.6081821133761315E-5	2.8040910566880658E-5	0.0	2.8040910566880657E-4	0.0014020455283440328
30	5.6081821133761315E-5	2.8040910566880658E-5	0.0	3.925727479363292E-4	0.0014300864389109134
31	5.6081821133761315E-5	2.8040910566880658E-5	0.0	5.608182113376131E-4	0.0015422500811784362
32	5.6081821133761315E-5	2.8040910566880658E-5	0.0	7.290636747388971E-4	0.0015702909917453167
33	5.6081821133761315E-5	2.8040910566880658E-5	0.0	9.253500487070616E-4	0.0015983319023121973
34	5.6081821133761315E-5	2.8040910566880658E-5	0.0	0.0013179227966433908	0.0015983319023121973
35	5.6081821133761315E-5	2.8040910566880658E-5	0.0	0.0021591501136498104	0.0016263728128790781
36	5.6081821133761315E-5	5.6081821133761315E-5	0.0	0.0032527456257581563	0.0016263728128790781
37	5.6081821133761315E-5	5.6081821133761315E-5	0.0	0.004682832064669069	0.0016263728128790781
38	5.6081821133761315E-5	8.412273170064196E-5	0.0	0.007514964031924016	0.0016824546340128393
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3615	0.0	28.001795	1
CGCAATA	590	0.0	25.356169	36
GGTATCA	1670	0.0	23.455238	1
TCTAGCG	695	0.0	22.157545	28
CTAGCGG	720	0.0	21.388186	29
TAGCGGC	745	0.0	21.261047	30
GCGCAAT	755	0.0	20.396969	35
CGAATGC	785	0.0	19.900227	43
CAATACG	765	0.0	19.55656	38
TATAGCG	80	8.99807E-5	19.249098	5
ATACGAA	780	0.0	19.18101	40
TCAACGC	5150	0.0	19.051535	4
TACGAAT	790	0.0	18.938211	41
AATACGA	810	0.0	18.742228	39
ATCAACG	5230	0.0	18.718054	3
CAACGCA	5245	0.0	18.706465	5
GTATTAC	450	0.0	18.093624	1
AACGCAG	5515	0.0	17.910313	6
TATCAAC	5545	0.0	17.73531	2
GCAATAC	880	0.0	17.25016	37
>>END_MODULE