FastQCFastQC Report
Thu 2 Feb 2017
SRR4062244_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062244_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930264
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT48690.2522452887273451No Hit
TATCAACGCAGAGTACTTTTTTTTT32010.16583223849172962No Hit
GGTATCAACGCAGAGTACTTTTTTT20210.10470070415238537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTAC759.7253E-713.9270761
GGTATCA9600.013.0566341
CGCGTAA957.376002E-813.00191810
TCGCGTA957.376002E-813.0019189
GCGTAAC1001.4418038E-712.35086211
GTATCAA26250.012.2268881
CCGTCGT1950.012.1812849
TACCGTC2450.012.02218157
AACCGCT658.0150296E-411.6940347
CGTCGTA1900.011.50169710
GTATTAG1001.940638E-611.394881
ATACCGT2350.011.3208196
AAGAGCG855.3175754E-511.177835
TTGCGCG855.3288313E-511.17550918
GGGTATA855.3485805E-511.1714521
ACCGTCG2250.010.9793988
GTCTTAG2250.010.9728481
ATTATCC1158.831521E-710.7379273
GTAAGAC1158.831521E-710.7379273
GTGTAGG1909.094947E-1210.4952841
Produced by FastQC (version 0.11.5)