##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062244_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1930264
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.243197821645122	32.0	32.0	32.0	32.0	32.0
2	30.80885567984483	32.0	32.0	32.0	32.0	32.0
3	30.839095584852643	32.0	32.0	32.0	32.0	32.0
4	30.83455579133217	32.0	32.0	32.0	32.0	32.0
5	30.76831977387549	32.0	32.0	32.0	32.0	32.0
6	34.42167962517044	36.0	36.0	36.0	32.0	36.0
7	34.355754964087815	36.0	36.0	36.0	32.0	36.0
8	34.29711220848547	36.0	36.0	36.0	32.0	36.0
9	34.421322679177564	36.0	36.0	36.0	32.0	36.0
10	34.13583116091892	36.0	36.0	36.0	32.0	36.0
11	34.422353626239726	36.0	36.0	36.0	32.0	36.0
12	34.266930844692745	36.0	36.0	36.0	32.0	36.0
13	34.34380737557142	36.0	36.0	36.0	32.0	36.0
14	34.247348031150146	36.0	36.0	36.0	32.0	36.0
15	34.20688361799215	36.0	36.0	36.0	32.0	36.0
16	34.20855230165407	36.0	36.0	36.0	32.0	36.0
17	34.14389223443011	36.0	36.0	36.0	32.0	36.0
18	34.150850350003935	36.0	36.0	36.0	32.0	36.0
19	34.15610507163787	36.0	36.0	36.0	32.0	36.0
20	34.13272744039158	36.0	36.0	36.0	32.0	36.0
21	34.106382339410565	36.0	36.0	36.0	32.0	36.0
22	34.08628767878383	36.0	36.0	36.0	32.0	36.0
23	34.05398484352399	36.0	36.0	36.0	32.0	36.0
24	34.03004148655314	36.0	36.0	36.0	32.0	36.0
25	33.682550158941986	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	34.0
5	121.0
6	426.0
7	108.0
8	312.0
9	257.0
10	150.0
11	31.0
12	66.0
13	85.0
14	393.0
15	490.0
16	743.0
17	1008.0
18	1378.0
19	1876.0
20	2739.0
21	3828.0
22	5600.0
23	8082.0
24	11509.0
25	15851.0
26	21877.0
27	27909.0
28	37316.0
29	49322.0
30	64575.0
31	87689.0
32	125252.0
33	180187.0
34	398352.0
35	882696.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.23250133273099	17.105945508528183	11.860243792633792	25.801309366107034
2	16.699123484592985	19.021508534873853	37.698399746769475	26.58096823376369
3	19.069345760016162	22.93386107016949	28.0246910382307	29.97210213158365
4	13.06219324728239	15.256072622256807	34.60955589278674	37.072178237674066
5	15.068500959780431	36.069398551415624	33.27432307494579	15.58777741385815
6	35.248377979924065	34.4598139212763	16.254258515475804	14.03754958332383
7	30.979518470922702	29.96897743785375	20.070280468439652	18.9812236227839
8	28.224161510300544	33.29801540193725	18.733958476277603	19.7438646114846
9	27.112811203319502	14.672302904564315	18.376192946058094	39.83869294605809
10	16.50969480229119	26.99174263669158	30.840250781487487	25.658311779529747
11	36.938603572102984	21.549083732965897	21.607342586962883	19.904970107968232
12	24.495236237688577	23.589532639306395	28.56047594568476	23.354755177320268
13	29.561917526981723	19.920959769778083	24.835583561001496	25.681539142238698
14	23.626270463829513	19.123449977075115	25.199373870612373	32.05090568848299
15	25.348860009368295	26.946034789257485	22.098176191358036	25.606929010016188
16	25.71222880533528	26.03282059048847	23.26570010223045	24.9892505019458
17	24.174175186638873	26.092614790751277	24.938262511793795	24.794947510816055
18	24.5740990836803	25.38466964154204	25.75367658499244	24.287554689785225
19	25.412984857913294	24.97744243932794	25.414799834059327	24.19477286869944
20	25.900928346270096	24.489456220251878	25.04014813716273	24.56946729631529
21	26.25062548775081	24.350957612023887	24.899416384278933	24.499000515946374
22	25.876227444452223	24.202313442704565	25.116592637537305	24.804866475305907
23	24.826641474763335	24.570764602610513	25.343787558421333	25.258806364204823
24	24.554949958283444	24.859281168540583	25.34256058234132	25.243208290834644
25	24.917840850286275	24.670248587816697	25.297914417595592	25.11399614430144
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	243.0
1	243.0
2	545.5
3	848.0
4	848.0
5	848.0
6	1712.5
7	2577.0
8	2577.0
9	2577.0
10	3103.5
11	3630.0
12	3630.0
13	3630.0
14	4733.0
15	5836.0
16	5836.0
17	5836.0
18	9455.5
19	13075.0
20	13075.0
21	13075.0
22	19659.5
23	26244.0
24	26244.0
25	26244.0
26	37094.5
27	47945.0
28	47945.0
29	47945.0
30	61008.5
31	74072.0
32	74072.0
33	74072.0
34	92357.5
35	110643.0
36	110643.0
37	110643.0
38	132885.5
39	155128.0
40	155128.0
41	155128.0
42	179448.5
43	203769.0
44	203769.0
45	203769.0
46	225432.0
47	247095.0
48	247095.0
49	247095.0
50	256817.0
51	266539.0
52	266539.0
53	266539.0
54	253939.0
55	241339.0
56	241339.0
57	241339.0
58	221702.5
59	202066.0
60	202066.0
61	202066.0
62	174954.5
63	147843.0
64	147843.0
65	147843.0
66	119733.0
67	91623.0
68	91623.0
69	91623.0
70	69268.5
71	46914.0
72	46914.0
73	46914.0
74	35076.5
75	23239.0
76	23239.0
77	23239.0
78	17473.5
79	11708.0
80	11708.0
81	11708.0
82	8121.5
83	4535.0
84	4535.0
85	4535.0
86	3211.0
87	1887.0
88	1887.0
89	1887.0
90	1217.0
91	547.0
92	547.0
93	547.0
94	372.5
95	198.0
96	198.0
97	198.0
98	459.5
99	721.0
100	721.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.807085455668239E-4
2	3.626446952333981E-4
3	4.6625746530008327E-4
4	0.008651666300568213
5	0.03398498858187274
6	0.061545985419610996
7	0.10309470621635175
8	0.10698018509385245
9	0.11728965571548762
10	0.13107015413435674
11	0.1378049841886913
12	0.12853164126772298
13	0.12355822830452208
14	0.1154246258542873
15	0.12873886680785634
16	0.11744507487058764
17	0.1213823601331217
18	0.10962231073055291
19	0.09656710170215058
20	0.09226717174438315
21	0.09164549512398304
22	0.10200677213065155
23	0.08299382882341483
24	0.09185272066411641
25	0.08755279070634897
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1930264.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.66085045667338
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.8753186810201	32.349954133551485
2	16.14037151998883	15.385276666586753
3	6.478404868310508	9.262988568789957
4	3.183463906124849	6.06906388656135
5	1.7815393288784842	4.245483976817985
6	1.1270387299957405	3.222937462152365
7	0.707347608503392	2.3598952012836
8	0.4861036794733087	1.8534491819052843
9	0.3675886063629568	1.5767627037697678
>10	1.7112475522355286	14.147132531033375
>50	0.08779481320478043	2.8701878271363777
>100	0.04877371070150224	4.50803958252481
>500	0.004245061147745083	1.3699876188995157
>1k	7.619340521593735E-4	0.7788406589873682
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4869	0.2522452887273451	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3201	0.16583223849172962	No Hit
GGTATCAACGCAGAGTACTTTTTTT	2021	0.10470070415238537	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.180638503334259E-5
2	0.0	0.0	0.0	0.0	5.180638503334259E-5
3	0.0	0.0	0.0	0.0	5.180638503334259E-5
4	0.0	0.0	0.0	0.0	5.180638503334259E-5
5	0.0	0.0	0.0	0.0	5.180638503334259E-5
6	0.0	0.0	0.0	0.0	1.0361277006668518E-4
7	0.0	0.0	0.0	5.180638503334259E-5	1.0361277006668518E-4
8	0.0	0.0	0.0	5.180638503334259E-5	1.0361277006668518E-4
9	0.0	0.0	0.0	1.0361277006668518E-4	1.0361277006668518E-4
10	0.0	0.0	0.0	1.0361277006668518E-4	1.0361277006668518E-4
11	0.0	0.0	0.0	1.0361277006668518E-4	1.0361277006668518E-4
12	0.0	0.0	0.0	1.0361277006668518E-4	2.5903192516671294E-4
13	0.0	0.0	0.0	1.0361277006668518E-4	2.5903192516671294E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTTAC	75	9.7253E-7	13.927076	1
GGTATCA	960	0.0	13.056634	1
CGCGTAA	95	7.376002E-8	13.001918	10
TCGCGTA	95	7.376002E-8	13.001918	9
GCGTAAC	100	1.4418038E-7	12.350862	11
GTATCAA	2625	0.0	12.226888	1
CCGTCGT	195	0.0	12.181284	9
TACCGTC	245	0.0	12.0221815	7
AACCGCT	65	8.0150296E-4	11.694034	7
CGTCGTA	190	0.0	11.501697	10
GTATTAG	100	1.940638E-6	11.39488	1
ATACCGT	235	0.0	11.320819	6
AAGAGCG	85	5.3175754E-5	11.17783	5
TTGCGCG	85	5.3288313E-5	11.175509	18
GGGTATA	85	5.3485805E-5	11.171452	1
ACCGTCG	225	0.0	10.979398	8
GTCTTAG	225	0.0	10.972848	1
ATTATCC	115	8.831521E-7	10.737927	3
GTAAGAC	115	8.831521E-7	10.737927	3
GTGTAGG	190	9.094947E-12	10.495284	1
>>END_MODULE