FastQCFastQC Report
Thu 2 Feb 2017
SRR4062244_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062244_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1930264
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30550.025.4328121
GGTATCA12450.024.3972341
TATGCGA856.1186056E-620.70507612
ACCGTAT1201.43336365E-820.1658828
TACCGTA1201.43336365E-820.1658827
ATACCGT3800.019.6834456
TATCAAC46000.017.5528552
TCAACGC45750.017.5512314
ATCAACG46000.017.4080183
TTAGACC1903.274181E-1117.3677464
ACCGTCG3300.017.332668
CAACGCA46350.017.2291055
AACGCAG46550.017.202346
CGTATAT2051.05501385E-1016.09693510
CGTCGTA3300.015.99937910
TATACCG1105.625901E-515.9993785
GTATTAG2950.015.6685261
CCGTATG1552.670804E-715.6122979
CCGTCGT3700.014.8642879
ACGCAGA54550.014.6795397
Produced by FastQC (version 0.11.5)