FastQCFastQC Report
Thu 2 Feb 2017
SRR4062243_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062243_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27981
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTACTTTTTTTTTTTTTT2881.0292698616918623No Hit
ACGCAGAGTACATGGGAGAAATCGT1600.5718165898288124No Hit
GTATCAACGCAGAGTACTTTTTTTT1340.4788963939816304No Hit
TATCAACGCAGAGTACTTTTTTTTT1320.47174868660877023No Hit
CCATTGGGATGTCCTGATCCAACAT910.32522068546513705No Hit
CCATAGGGTCTTCTCGTCTTATTAT820.29305600228726636No Hit
ATCCTAGCCCTAGCCCTACACAAAT810.2894821486008363No Hit
GGTATCAACGCAGAGTACTTTTTTT700.25016975805010544No Hit
GTCCAACTGTCTCTTATCTTTAATC680.24302205067724528No Hit
AGCATGAACGGCTAAACGAGGGTCC640.22872663593152495No Hit
CTCCGAGGTCACCCCAACCGAAATT610.21800507487223475No Hit
AAATATATCTGGGTCAATAAGATAT570.20370966012651445No Hit
ACCCTATGGAGCTTAAATTATATAA550.19656195275365426No Hit
TACTAACAGTGTTGCATCTATAAAG540.1929880990672242No Hit
ATCCTGACCGTGCAAAGGTAGCATA540.1929880990672242No Hit
ACGCAGAGTACATGGGCAGAAATCA520.18584039169436403No Hit
ACGCAGAGTACATGGGGAATAATTG500.17869268432150387No Hit
GTTATAGATTAACCCAATTTTAAGT480.17154497694864373No Hit
GTATTAGAGGCACTGCCTGCCCAGT440.1572495622029234No Hit
GATTAAACCTTGTACCTTTTGCATA440.1572495622029234No Hit
CTATAGAACTAGTACCGCAAGGGAA440.1572495622029234No Hit
CCAATAAAGAAAGCGTTCAAGCTCA440.1572495622029234No Hit
ATCTATAACTTTATAGATGCAACAC440.1572495622029234No Hit
ATTTTAAACTTGCTAAAAAAACAAC430.15367570851649334No Hit
AAGAGGGACAGCTCTTCTGGAACGG430.15367570851649334No Hit
GTATCCTGACCGTGCAAAGGTAGCA430.15367570851649334No Hit
CTATTATATAAATCAAAACATTTAT420.15010185483006325No Hit
CCTATAGTCTGATTAACTAACAATG400.1429541474572031No Hit
CCTTTAATAGTGAATAATTAACAAA390.13938029377077302No Hit
CTTCTACACCATTGGGATGTCCTGA380.13580644008434295No Hit
AATCTAAACTTACTTTTTGATTTTG370.13223258639791288No Hit
ACGCAGAGTACATGGGACTAATCCT370.13223258639791288No Hit
GTTTAGATTATAGCCAAAAGAGGGA370.13223258639791288No Hit
ACCTATAACTTCTCTGTTAACCCAA360.1286587327114828No Hit
CGTCTATGTGGCAAAATAGTGAGAA360.1286587327114828No Hit
CCTCTAGCATTACAAGTATTAGAGG360.1286587327114828No Hit
GTTATCCCTAGGGTAACTTGGTCCG330.11793717165219256No Hit
GCGCAAGACGGACCAGAGCGAAAGC320.11436331796576248No Hit
ACGTAGGACTTTAATCGTTGAACAA320.11436331796576248No Hit
TTATATAACTTATCTATTTAATTTA320.11436331796576248No Hit
CTTTTAGGCCTACAATGGTTAAAAG320.11436331796576248No Hit
CTGTTAGTATGAGTAACAAGAATTC320.11436331796576248No Hit
CTATTAAAGGTTTTTTCCGTTCCAG320.11436331796576248No Hit
AGCCTAACGAGCTTGGTGATAGCTG310.11078946427933241No Hit
TCTATAAAGTTATAGATTAACCCAA300.10721561059290233No Hit
CATACTAACAGTGTTGCATCTATAA290.10364175690647225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA280.10006790322004218No Hit
CATTTATCCTACTAAAAGTATTGGA280.10006790322004218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT250.005887816719.03
TTCTCGT250.005887816719.011
CTTATTA250.005887816719.018
GTCTTCT250.005887816719.08
TAGGGTC250.005887816719.04
TCTTCTC250.005887816719.09
CGTCTTA250.005887816719.015
CTTCTCG250.005887816719.010
GAGTACA2250.013.088896
CGCAGAG2850.013.02
CAGAGTA2800.012.8928584
AGAGTAC2800.012.8928585
AGTACAT2300.012.8043477
ACGCAGA2900.012.7758631
GCAGAGT2900.012.7758633
TACATGG2450.011.6326539
ACATGGG2500.011.39999910
GTACATG2500.011.3999998
CATGGGA854.901787E-511.1764711
CATGGGG803.5266587E-410.687511
Produced by FastQC (version 0.11.5)