##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062243_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27981
Sequences flagged as poor quality	0
Sequence length	25
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	27.526071262642507	32.0	21.0	32.0	14.0	32.0
2	25.741360208713054	32.0	14.0	32.0	14.0	32.0
3	26.079411028912478	32.0	21.0	32.0	14.0	32.0
4	25.835102390908116	32.0	14.0	32.0	14.0	32.0
5	26.13684285765341	32.0	21.0	32.0	14.0	32.0
6	29.255816446874665	36.0	21.0	36.0	14.0	36.0
7	29.094028090489974	32.0	21.0	36.0	14.0	36.0
8	29.040849147635896	32.0	21.0	36.0	14.0	36.0
9	29.10503555984418	32.0	21.0	36.0	14.0	36.0
10	28.062578178049392	32.0	21.0	36.0	14.0	36.0
11	29.714913691433473	36.0	21.0	36.0	14.0	36.0
12	28.636610557163788	32.0	21.0	36.0	14.0	36.0
13	29.110038955005184	32.0	21.0	36.0	14.0	36.0
14	28.503877631249775	32.0	21.0	36.0	14.0	36.0
15	28.527036203137843	32.0	21.0	36.0	14.0	36.0
16	28.489439262356598	32.0	21.0	36.0	14.0	36.0
17	28.289482148600836	32.0	21.0	36.0	14.0	36.0
18	28.39255208891748	32.0	21.0	36.0	14.0	36.0
19	28.389835960115793	32.0	21.0	36.0	14.0	36.0
20	28.286658804188555	32.0	21.0	36.0	14.0	36.0
21	28.406061255852187	32.0	21.0	36.0	14.0	36.0
22	28.043743969121905	32.0	21.0	36.0	14.0	36.0
23	28.022551016761373	32.0	21.0	36.0	14.0	36.0
24	27.834923698223793	32.0	21.0	36.0	14.0	36.0
25	27.013652121082163	32.0	14.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	0.0
5	1.0
6	2.0
7	2.0
8	6.0
9	3.0
10	1.0
11	1.0
12	1.0
13	2.0
14	39.0
15	99.0
16	170.0
17	380.0
18	646.0
19	983.0
20	1199.0
21	1273.0
22	1348.0
23	1331.0
24	1350.0
25	1315.0
26	1376.0
27	1399.0
28	1461.0
29	1505.0
30	1557.0
31	1666.0
32	1830.0
33	2042.0
34	2586.0
35	2406.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.16536221007112	27.50795182445231	11.654336871448482	27.672349094028093
2	13.619956398985025	20.503198599049355	33.98377470426361	31.893070297702014
3	23.838456040028593	22.72337383845604	28.370264474624733	25.067905646890637
4	10.332380271622588	16.39385275196569	34.31022158684775	38.963545389563976
5	11.985273091221046	41.857306262510726	32.68515870746354	13.472261938804689
6	34.18888968327733	38.06034174590691	15.543004218202617	12.20776435261314
7	25.84233493096788	37.64933113956649	18.94269976393161	17.565634165534018
8	30.37304624628921	32.107729174863195	18.419829035373226	19.099395543474373
9	23.60137358706539	14.340392044641579	25.321934468450426	36.736299899842614
10	15.095149520675347	30.12233509801116	30.57304335384175	24.20947202747174
11	31.133054273550144	22.954456012307254	22.721906192980573	23.190583521162033
12	21.04453586120551	30.10552673940261	27.862636379896262	20.987301019495618
13	23.827580039348952	19.774637810767302	31.876229654802362	24.52155249508138
14	23.602417826102506	21.2275117135806	26.917987052469687	28.252083407847206
15	26.532875438219932	26.844101023109395	23.223867782786005	23.399155755884667
16	26.35495295674883	28.254570171359067	23.421457446427933	21.96901942546417
17	20.26828832051511	29.40439992845645	27.18297263459131	23.14433911643713
18	20.964338090639195	26.816181993776155	29.337911793110848	22.8815681224738
19	21.834763948497855	27.86480686695279	28.372675250357656	21.927753934191703
20	22.194406694800087	27.827050997782703	27.787711894714256	22.19083041270295
21	23.394134477825464	26.258941344778254	28.100858369098713	22.24606580829757
22	21.32484440947135	27.41254739251735	27.341011517275916	23.921596680735387
23	20.977720559310516	26.206058005221184	28.995458284161213	23.820763151307084
24	21.382590658751162	28.50296831414062	27.480151634360915	22.634289392747302
25	21.477614075239593	26.75582892290087	28.972965241024173	22.793591760835362
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	34.0
1	34.0
2	24.5
3	15.0
4	15.0
5	15.0
6	72.5
7	130.0
8	130.0
9	130.0
10	147.5
11	165.0
12	165.0
13	165.0
14	189.5
15	214.0
16	214.0
17	214.0
18	386.0
19	558.0
20	558.0
21	558.0
22	878.5
23	1199.0
24	1199.0
25	1199.0
26	1430.0
27	1661.0
28	1661.0
29	1661.0
30	1744.0
31	1827.0
32	1827.0
33	1827.0
34	2009.0
35	2191.0
36	2191.0
37	2191.0
38	2202.0
39	2213.0
40	2213.0
41	2213.0
42	2365.0
43	2517.0
44	2517.0
45	2517.0
46	2985.0
47	3453.0
48	3453.0
49	3453.0
50	3336.5
51	3220.0
52	3220.0
53	3220.0
54	2968.5
55	2717.0
56	2717.0
57	2717.0
58	2401.5
59	2086.0
60	2086.0
61	2086.0
62	1815.0
63	1544.0
64	1544.0
65	1544.0
66	1262.0
67	980.0
68	980.0
69	980.0
70	801.0
71	622.0
72	622.0
73	622.0
74	465.5
75	309.0
76	309.0
77	309.0
78	252.5
79	196.0
80	196.0
81	196.0
82	130.0
83	64.0
84	64.0
85	64.0
86	46.0
87	28.0
88	28.0
89	28.0
90	21.5
91	15.0
92	15.0
93	15.0
94	9.0
95	3.0
96	3.0
97	3.0
98	11.5
99	20.0
100	20.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0035738536864300774
4	0.0035738536864300774
5	0.01786926843215039
6	0.025016975805010545
7	0.08219863478789179
8	0.0786247811014617
9	0.08934634216075193
10	0.08934634216075193
11	0.10721561059290233
12	0.09292019584718202
13	0.09292019584718202
14	0.0786247811014617
15	0.0964940495336121
16	0.10006790322004218
17	0.09292019584718202
18	0.08577248847432187
19	0.07505092741503162
20	0.06790322004217147
21	0.07505092741503162
22	0.08219863478789179
23	0.0643293663557414
24	0.06790322004217147
25	0.06075551266931132
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	27981.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.44612415567707
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.09045663804902	45.434401915585575
2	11.303969142714944	12.987384296486901
3	3.315913898220729	5.7145920446016945
4	1.8912529550827424	4.345806082698974
5	1.0700510140599726	3.0735141703298665
6	0.6781137240263779	2.337300310925271
7	0.4541495582928954	1.8262392337657696
8	0.36083115590394427	1.658268110503556
9	0.23018539255941273	1.1900932775812159
>10	1.5055368918750778	16.06089846681677
>50	0.07465472191116088	2.8197705585933313
>100	0.024884907303720297	2.5517315321110754
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ACGCAGAGTACTTTTTTTTTTTTTT	288	1.0292698616918623	No Hit
ACGCAGAGTACATGGGAGAAATCGT	160	0.5718165898288124	No Hit
GTATCAACGCAGAGTACTTTTTTTT	134	0.4788963939816304	No Hit
TATCAACGCAGAGTACTTTTTTTTT	132	0.47174868660877023	No Hit
CCATTGGGATGTCCTGATCCAACAT	91	0.32522068546513705	No Hit
CCATAGGGTCTTCTCGTCTTATTAT	82	0.29305600228726636	No Hit
ATCCTAGCCCTAGCCCTACACAAAT	81	0.2894821486008363	No Hit
GGTATCAACGCAGAGTACTTTTTTT	70	0.25016975805010544	No Hit
GTCCAACTGTCTCTTATCTTTAATC	68	0.24302205067724528	No Hit
AGCATGAACGGCTAAACGAGGGTCC	64	0.22872663593152495	No Hit
CTCCGAGGTCACCCCAACCGAAATT	61	0.21800507487223475	No Hit
AAATATATCTGGGTCAATAAGATAT	57	0.20370966012651445	No Hit
ACCCTATGGAGCTTAAATTATATAA	55	0.19656195275365426	No Hit
TACTAACAGTGTTGCATCTATAAAG	54	0.1929880990672242	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	54	0.1929880990672242	No Hit
ACGCAGAGTACATGGGCAGAAATCA	52	0.18584039169436403	No Hit
ACGCAGAGTACATGGGGAATAATTG	50	0.17869268432150387	No Hit
GTTATAGATTAACCCAATTTTAAGT	48	0.17154497694864373	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	44	0.1572495622029234	No Hit
GATTAAACCTTGTACCTTTTGCATA	44	0.1572495622029234	No Hit
CTATAGAACTAGTACCGCAAGGGAA	44	0.1572495622029234	No Hit
CCAATAAAGAAAGCGTTCAAGCTCA	44	0.1572495622029234	No Hit
ATCTATAACTTTATAGATGCAACAC	44	0.1572495622029234	No Hit
ATTTTAAACTTGCTAAAAAAACAAC	43	0.15367570851649334	No Hit
AAGAGGGACAGCTCTTCTGGAACGG	43	0.15367570851649334	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	43	0.15367570851649334	No Hit
CTATTATATAAATCAAAACATTTAT	42	0.15010185483006325	No Hit
CCTATAGTCTGATTAACTAACAATG	40	0.1429541474572031	No Hit
CCTTTAATAGTGAATAATTAACAAA	39	0.13938029377077302	No Hit
CTTCTACACCATTGGGATGTCCTGA	38	0.13580644008434295	No Hit
AATCTAAACTTACTTTTTGATTTTG	37	0.13223258639791288	No Hit
ACGCAGAGTACATGGGACTAATCCT	37	0.13223258639791288	No Hit
GTTTAGATTATAGCCAAAAGAGGGA	37	0.13223258639791288	No Hit
ACCTATAACTTCTCTGTTAACCCAA	36	0.1286587327114828	No Hit
CGTCTATGTGGCAAAATAGTGAGAA	36	0.1286587327114828	No Hit
CCTCTAGCATTACAAGTATTAGAGG	36	0.1286587327114828	No Hit
GTTATCCCTAGGGTAACTTGGTCCG	33	0.11793717165219256	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	32	0.11436331796576248	No Hit
ACGTAGGACTTTAATCGTTGAACAA	32	0.11436331796576248	No Hit
TTATATAACTTATCTATTTAATTTA	32	0.11436331796576248	No Hit
CTTTTAGGCCTACAATGGTTAAAAG	32	0.11436331796576248	No Hit
CTGTTAGTATGAGTAACAAGAATTC	32	0.11436331796576248	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	32	0.11436331796576248	No Hit
AGCCTAACGAGCTTGGTGATAGCTG	31	0.11078946427933241	No Hit
TCTATAAAGTTATAGATTAACCCAA	30	0.10721561059290233	No Hit
CATACTAACAGTGTTGCATCTATAA	29	0.10364175690647225	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	28	0.10006790322004218	No Hit
CATTTATCCTACTAAAAGTATTGGA	28	0.10006790322004218	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAGGGT	25	0.0058878167	19.0	3
TTCTCGT	25	0.0058878167	19.0	11
CTTATTA	25	0.0058878167	19.0	18
GTCTTCT	25	0.0058878167	19.0	8
TAGGGTC	25	0.0058878167	19.0	4
TCTTCTC	25	0.0058878167	19.0	9
CGTCTTA	25	0.0058878167	19.0	15
CTTCTCG	25	0.0058878167	19.0	10
GAGTACA	225	0.0	13.08889	6
CGCAGAG	285	0.0	13.0	2
CAGAGTA	280	0.0	12.892858	4
AGAGTAC	280	0.0	12.892858	5
AGTACAT	230	0.0	12.804347	7
ACGCAGA	290	0.0	12.775863	1
GCAGAGT	290	0.0	12.775863	3
TACATGG	245	0.0	11.632653	9
ACATGGG	250	0.0	11.399999	10
GTACATG	250	0.0	11.399999	8
CATGGGA	85	4.901787E-5	11.17647	11
CATGGGG	80	3.5266587E-4	10.6875	11
>>END_MODULE