FastQCFastQC Report
Thu 2 Feb 2017
SRR4062243_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062243_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27981
Sequences flagged as poor quality0
Sequence length50
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT800.2859082949144062No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA650.23230048961795502No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA610.21800507487223475No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT600.21443122118580465No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC580.2072835138129445No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA560.20013580644008436No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT550.19656195275365426No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG530.1894142453807941No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG530.1894142453807941No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA500.17869268432150387No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAAAACTAT480.17154497694864373No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA460.16439726957578357No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC460.16439726957578357No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT440.1572495622029234No Hit
CTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTATCCTGACCGTGCA430.15367570851649334No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA420.15010185483006325No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT410.14652800114363318No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC400.1429541474572031No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG390.13938029377077302No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG390.13938029377077302No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT380.13580644008434295No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC350.12508487902505272No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT340.12151102533862264No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA330.11793717165219256No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAA330.11793717165219256No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAATTTATTAAACCT320.11436331796576248No Hit
ATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCG320.11436331796576248No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG310.11078946427933241No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC310.11078946427933241No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT300.10721561059290233No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC300.10721561059290233No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA300.10721561059290233No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT290.10364175690647225No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280.10006790322004218No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC280.10006790322004218No Hit
TCTTTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAA280.10006790322004218No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGG250.002317046535.1899816
TTTAAGT406.872031E-427.49217443
TTAAGTT500.002522654621.99373844
ACATGGG1056.869502E-414.6624923
TACATGG1200.001883817712.829682
Produced by FastQC (version 0.11.5)