##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062243_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	27981
Sequences flagged as poor quality	0
Sequence length	50
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.033522747578715	32.0	32.0	32.0	21.0	32.0
2	28.916193131053216	32.0	32.0	32.0	14.0	32.0
3	30.52900182266538	32.0	32.0	32.0	32.0	32.0
4	30.981344483756835	32.0	32.0	32.0	32.0	32.0
5	30.774418355312534	32.0	32.0	32.0	32.0	32.0
6	34.244201422393765	36.0	36.0	36.0	32.0	36.0
7	34.28891033201101	36.0	36.0	36.0	32.0	36.0
8	34.12955219613309	36.0	36.0	36.0	32.0	36.0
9	34.50241235123834	36.0	36.0	36.0	32.0	36.0
10	34.13276866445088	36.0	36.0	36.0	32.0	36.0
11	34.62767592294772	36.0	36.0	36.0	32.0	36.0
12	34.319502519566846	36.0	36.0	36.0	32.0	36.0
13	34.51524248597262	36.0	36.0	36.0	32.0	36.0
14	34.39308816697044	36.0	36.0	36.0	32.0	36.0
15	34.303134269683	36.0	36.0	36.0	32.0	36.0
16	34.40055752117508	36.0	36.0	36.0	32.0	36.0
17	34.30717272434867	36.0	36.0	36.0	32.0	36.0
18	34.314141739037204	36.0	36.0	36.0	32.0	36.0
19	34.319502519566846	36.0	36.0	36.0	32.0	36.0
20	34.27372145384368	36.0	36.0	36.0	32.0	36.0
21	34.315499803438044	36.0	36.0	36.0	32.0	36.0
22	34.34162467388585	36.0	36.0	36.0	32.0	36.0
23	34.22472391980272	36.0	36.0	36.0	32.0	36.0
24	34.20188699474644	36.0	36.0	36.0	32.0	36.0
25	34.203388013294735	36.0	36.0	36.0	32.0	36.0
26	34.0503555984418	36.0	36.0	36.0	32.0	36.0
27	34.02830492119653	36.0	36.0	36.0	32.0	36.0
28	34.0548586540867	36.0	36.0	36.0	32.0	36.0
29	33.988563668203426	36.0	36.0	36.0	32.0	36.0
30	33.93942318001501	36.0	36.0	36.0	32.0	36.0
31	33.984668167685214	36.0	36.0	36.0	32.0	36.0
32	33.86530145455845	36.0	36.0	36.0	32.0	36.0
33	33.86937564776098	36.0	36.0	36.0	32.0	36.0
34	33.73853686430078	36.0	36.0	36.0	32.0	36.0
35	33.76101640398842	36.0	36.0	36.0	32.0	36.0
36	33.70751581430256	36.0	36.0	36.0	32.0	36.0
37	33.73271148279189	36.0	36.0	36.0	32.0	36.0
38	33.69565062006362	36.0	36.0	36.0	27.0	36.0
39	33.607162002787604	36.0	36.0	36.0	27.0	36.0
40	33.66516564811837	36.0	36.0	36.0	27.0	36.0
41	33.5493013116043	36.0	36.0	36.0	21.0	36.0
42	33.585432972374115	36.0	36.0	36.0	27.0	36.0
43	33.51102533862264	36.0	36.0	36.0	21.0	36.0
44	33.49815946535149	36.0	36.0	36.0	21.0	36.0
45	33.405882563167864	36.0	36.0	36.0	21.0	36.0
46	33.329437832815124	36.0	36.0	36.0	21.0	36.0
47	33.41045709588649	36.0	36.0	36.0	21.0	36.0
48	33.26614488402845	36.0	36.0	36.0	21.0	36.0
49	33.17862120724777	36.0	36.0	36.0	21.0	36.0
50	32.625853257567634	36.0	36.0	36.0	14.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
22	7.0
23	18.0
24	36.0
25	69.0
26	166.0
27	252.0
28	426.0
29	780.0
30	1221.0
31	1969.0
32	3082.0
33	5063.0
34	7935.0
35	6957.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.07577653072166	22.665046288022303	13.01068735032348	24.248489830932552
2	17.305218012866334	22.119370979270908	37.29807005003574	23.27734095782702
3	19.40602551731532	25.059862049247705	32.16110932418427	23.373003109252707
4	13.666416496908617	19.241628247739538	38.58689825238555	28.5050570029663
5	16.464743933383367	36.628426432221865	33.12604981952039	13.78077981487438
6	30.33129623673207	40.877738465387225	16.12880168685894	12.662163611021764
7	27.390014652800115	34.23037060862728	19.87777420392409	18.50184053464851
8	27.07194167470784	34.26610914549158	18.45895429041135	20.202994889389227
9	24.90618634073121	15.035202458811337	21.393088166970443	38.66552303348701
10	16.55766412923055	25.760337371788	32.03959829884565	25.642400200135807
11	35.16672027447196	22.661806225653123	23.04420857010114	19.127264929773773
12	23.054930131160432	26.485829670133302	28.14767163432329	22.311568564382974
13	25.78178049390658	20.356670597905723	27.797433973053142	26.06411493513456
14	22.018512562095708	21.864836853579213	25.989064007719527	30.12758657660555
15	25.06700975662056	27.114827918945	23.15142418069404	24.666738143740396
16	24.89189092598549	28.365676709195526	22.96915764268611	23.773274722132875
17	21.471712948071904	27.339980701190093	27.129123333690718	24.059183017047282
18	22.236517636967942	26.081984203566705	27.893928022586756	23.787570136878596
19	23.812587112683605	27.993995925806797	26.056967227761696	22.136449733747902
20	23.687502233658552	27.2220435295379	26.17847825310032	22.91197598370323
21	24.838283120689038	24.78824916907902	27.068367821021404	23.30509988921054
22	22.987026911118257	26.86108430720846	25.88542225081305	24.266466530860225
23	23.212179693363353	26.09627961831243	27.407883921232262	23.283656767091955
24	23.00847003323684	28.49433544190701	25.5709231264072	22.926271398448947
25	23.658911404167114	25.763911225474427	26.986169186233518	23.59100818412494
26	22.56888602980594	26.439369572209714	27.51152567813874	23.48021871984561
27	23.11651179413867	27.07648320228735	26.844174410293064	22.962830593280913
28	22.622493835102393	26.864658160894894	27.472213287588005	23.040634716414708
29	23.573138915692795	27.361423823308673	27.114827918945	21.950609342053536
30	23.919802723276508	26.32858010793038	27.479360994960867	22.272256173832243
31	22.851220471033916	26.15346127729531	27.50795182445231	23.48736642721847
32	22.175762124298632	26.857510453522032	27.400736213859407	23.565991208319932
33	22.876237446838925	27.036203137843533	27.59729816661306	22.490261248704478
34	22.20077910010364	26.85036274614917	28.14052392695043	22.808334226796756
35	24.112790822343733	26.425074157463996	25.867552982380904	23.59458203781137
36	22.511704370823058	26.239233765769633	27.057646259962116	24.191415603445197
37	24.209284871877344	26.392909474286125	25.445838247382152	23.95196740645438
38	22.783317250991743	26.77888567242057	27.21846967585147	23.219327400736216
39	22.55101676137379	27.361423823308673	25.84968371394875	24.237875701368786
40	22.654658518280264	26.596619134412634	28.47289231978843	22.275830027518673
41	22.858368178406778	27.09338479682642	27.314963725385084	22.733283299381725
42	22.66538007933955	27.74382616775669	26.446517279582576	23.14427647332118
43	22.5553967119371	26.74767691208006	27.601858470335955	23.09506790564689
44	23.198713366690495	26.915654038599	27.294496068620443	22.591136526090065
45	23.05850398484686	26.58947142703978	27.61159358135878	22.740431006754584
46	24.656016582681104	26.818198062971305	25.317179514670666	23.208605839676924
47	22.97273149637254	27.200600407419316	26.660948500768377	23.165719595439764
48	24.4773238983596	28.004717486866088	25.85325756763518	21.66470104713913
49	22.318716271755832	27.5544119223759	26.650226939709086	23.47664486615918
50	22.515278224509487	27.829598656231013	26.650226939709086	23.00489617955041
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28.0
1	15.0
2	2.0
3	5.0
4	8.0
5	10.0
6	12.0
7	8.5
8	5.0
9	7.5
10	10.0
11	8.5
12	7.0
13	28.5
14	50.0
15	68.0
16	86.0
17	149.0
18	212.0
19	223.0
20	234.0
21	283.5
22	333.0
23	334.0
24	335.0
25	502.0
26	669.0
27	842.0
28	1015.0
29	1007.0
30	999.0
31	863.0
32	727.0
33	739.5
34	752.0
35	969.5
36	1187.0
37	1348.5
38	1510.0
39	1530.5
40	1551.0
41	1595.0
42	1639.0
43	1500.0
44	1361.0
45	1394.0
46	1427.0
47	1419.5
48	1412.0
49	1379.0
50	1346.0
51	1400.5
52	1455.0
53	1429.5
54	1404.0
55	1388.0
56	1372.0
57	1425.0
58	1478.0
59	1354.5
60	1231.0
61	1158.5
62	1086.0
63	934.5
64	783.0
65	705.5
66	628.0
67	561.0
68	494.0
69	423.5
70	353.0
71	289.0
72	225.0
73	202.5
74	180.0
75	149.0
76	118.0
77	104.5
78	91.0
79	76.0
80	61.0
81	51.5
82	42.0
83	34.5
84	27.0
85	19.5
86	12.0
87	7.5
88	3.0
89	3.5
90	4.0
91	3.0
92	2.0
93	2.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	5.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01429541474572031
2	0.0035738536864300774
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0035738536864300774
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0035738536864300774
44	0.0035738536864300774
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	27981.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.29430685107752
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.62342638204707	54.601336621278726
2	8.981937602627257	11.729387798863515
3	2.796934865900383	5.478717701297309
4	1.2151067323481117	3.173582073549909
5	0.6787082649151615	2.215789285586648
6	0.5582922824302134	2.1871984560952074
7	0.3886152162014231	1.7762052821557486
8	0.2955665024630542	1.5439047925377936
9	0.19704433497536944	1.1579285944033453
>10	1.2151067323481117	14.20249454987313
>50	0.04926108374384236	1.9334548443586719
>100	0.0	0.0
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT	80	0.2859082949144062	No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA	65	0.23230048961795502	No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA	61	0.21800507487223475	No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT	60	0.21443122118580465	No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC	58	0.2072835138129445	No Hit
GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA	56	0.20013580644008436	No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT	55	0.19656195275365426	No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG	53	0.1894142453807941	No Hit
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG	53	0.1894142453807941	No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA	50	0.17869268432150387	No Hit
TTATATAACTTATCTATTTAATTTATTAAACCTAATGGCCCAAAAACTAT	48	0.17154497694864373	No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA	46	0.16439726957578357	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	46	0.16439726957578357	No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT	44	0.1572495622029234	No Hit
CTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTATCCTGACCGTGCA	43	0.15367570851649334	No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA	42	0.15010185483006325	No Hit
CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT	41	0.14652800114363318	No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC	40	0.1429541474572031	No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG	39	0.13938029377077302	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	39	0.13938029377077302	No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT	38	0.13580644008434295	No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC	35	0.12508487902505272	No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT	34	0.12151102533862264	No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA	33	0.11793717165219256	No Hit
CTATTATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAA	33	0.11793717165219256	No Hit
ACCCTATGGAGCTTAAATTATATAACTTATCTATTTAATTTATTAAACCT	32	0.11436331796576248	No Hit
ATATAAATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCG	32	0.11436331796576248	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	31	0.11078946427933241	No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC	31	0.11078946427933241	No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT	30	0.10721561059290233	No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC	30	0.10721561059290233	No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA	30	0.10721561059290233	No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT	29	0.10364175690647225	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28	0.10006790322004218	No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC	28	0.10006790322004218	No Hit
TCTTTAATCAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAA	28	0.10006790322004218	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0	0.0
22	0.0	0.0	0.0	0.0	0.0
23	0.0	0.0	0.0	0.0	0.0
24	0.0	0.0	0.0	0.0	0.0
25	0.0	0.0	0.0	0.0	0.0
26	0.0	0.0	0.0	0.0	0.0
27	0.0	0.0	0.0	0.0	0.0
28	0.0	0.0	0.0	0.0	0.0035738536864300774
29	0.0	0.0	0.0	0.0	0.0035738536864300774
30	0.0	0.0	0.0	0.0	0.0035738536864300774
31	0.0	0.0	0.0	0.0	0.007147707372860155
32	0.0	0.0	0.0	0.0	0.007147707372860155
33	0.0	0.0	0.0	0.0	0.007147707372860155
34	0.0	0.0	0.0	0.0	0.007147707372860155
35	0.0	0.0	0.0	0.0035738536864300774	0.007147707372860155
36	0.0	0.0	0.0	0.007147707372860155	0.007147707372860155
37	0.0	0.0	0.0	0.007147707372860155	0.007147707372860155
38	0.0	0.0	0.0	0.01429541474572031	0.007147707372860155
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGGGG	25	0.0023170465	35.18998	16
TTTAAGT	40	6.872031E-4	27.492174	43
TTAAGTT	50	0.0025226546	21.993738	44
ACATGGG	105	6.869502E-4	14.662492	3
TACATGG	120	0.0018838177	12.82968	2
>>END_MODULE