FastQCFastQC Report
Thu 2 Feb 2017
SRR4062242_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062242_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264569
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT20630.16313858713917548No Hit
GTATCAACGCAGAGTACTTTTTTTT19990.15807757425652533No Hit
GTCCTACAGTGGACATTTCTAAATT19250.1522257781109611No Hit
CTGTAGGACGTGGAATATGGCAAGA19210.15190946480579548No Hit
CTTTAGGACGTGAAATATGGCGAGG17520.13854522766254748No Hit
GTCCTACAGTGTGCATTTCTCATTT13540.10707205379856695No Hit
TATCAACGCAGAGTACTTTTTTTTT12660.100113161084923No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCCG456.750837E-414.78073510
GCTCGTA903.6327037E-813.7233379
TTAGACA1800.013.7217074
GTCGGCG655.4467528E-513.15491512
AGCTCGT957.370727E-813.0015728
TCGCGTA905.3969416E-712.6676969
GCGTTAT751.4840603E-512.6616791
AAGACGG2200.012.5227535
CGCAAGA1900.012.4980472
AGGCCCG1750.012.48821310
CGTCGTA1750.012.48821310
CCAACGA1155.322363E-912.39084119
GTCCTAT2550.012.2892761
TAGGCCC550.003068759612.0904564
TCCAACG1501.4551915E-1112.03288318
GCGTAAC951.0367403E-612.00240111
GTCGCGT951.0377316E-612.0014518
CGCATCG1900.012.000513
GGTCGGC802.8594994E-511.877377511
AAGCTCG802.8618186E-511.8764367
Produced by FastQC (version 0.11.5)