FastQCFastQC Report
Thu 2 Feb 2017
SRR4062242_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062242_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264569
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA14950.027.9666791
CGTCCGG450.0013985224.44363210
TTACTCG551.5923248E-424.00299843
GGTATCA6250.021.8293151
TGTAGGA15750.021.5112482
GTAGGAC15300.020.848983
TAGGACA5350.020.1488634
TAGGACG14450.019.9439744
GTATTAC1001.1208995E-619.8048231
CTGTAGG16450.019.6643641
TCGATCG901.0012807E-519.55722641
ATCAACG21050.019.5432913
TCAACGC21100.019.4969834
GTCCTAC14300.019.3893361
AACGCAG21700.019.0592756
ATTTCGT7450.018.90094242
AGACCGT708.125423E-418.8565166
GGACGTG14700.018.8565146
CAACGCA21950.018.7419745
AGGACGT15100.018.6483865