##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062242_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1264569
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27144979831073	32.0	32.0	32.0	32.0	32.0
2	31.451830623714482	32.0	32.0	32.0	32.0	32.0
3	31.581381482544646	32.0	32.0	32.0	32.0	32.0
4	31.655314972927535	32.0	32.0	32.0	32.0	32.0
5	31.63673631094863	32.0	32.0	32.0	32.0	32.0
6	35.280017934964405	36.0	36.0	36.0	36.0	36.0
7	35.28470095344738	36.0	36.0	36.0	36.0	36.0
8	35.23741606824143	36.0	36.0	36.0	36.0	36.0
9	35.31745994089686	36.0	36.0	36.0	36.0	36.0
10	35.20542334977372	36.0	36.0	36.0	36.0	36.0
11	35.322283718800634	36.0	36.0	36.0	36.0	36.0
12	35.243234651489956	36.0	36.0	36.0	36.0	36.0
13	35.273502671661255	36.0	36.0	36.0	36.0	36.0
14	35.24639462140856	36.0	36.0	36.0	36.0	36.0
15	35.22136000487122	36.0	36.0	36.0	36.0	36.0
16	35.23512833226182	36.0	36.0	36.0	36.0	36.0
17	35.220097124000354	36.0	36.0	36.0	36.0	36.0
18	35.206914767007575	36.0	36.0	36.0	36.0	36.0
19	35.19525387701264	36.0	36.0	36.0	36.0	36.0
20	35.19277951618298	36.0	36.0	36.0	36.0	36.0
21	35.172495134705976	36.0	36.0	36.0	36.0	36.0
22	35.148086818512866	36.0	36.0	36.0	36.0	36.0
23	35.127501939395955	36.0	36.0	36.0	36.0	36.0
24	35.112441472153755	36.0	36.0	36.0	36.0	36.0
25	35.08048433893287	36.0	36.0	36.0	36.0	36.0
26	35.04647275079493	36.0	36.0	36.0	36.0	36.0
27	35.02273343724225	36.0	36.0	36.0	36.0	36.0
28	34.98563700359569	36.0	36.0	36.0	36.0	36.0
29	34.95533814287714	36.0	36.0	36.0	36.0	36.0
30	34.92922331640266	36.0	36.0	36.0	36.0	36.0
31	34.930192816683	36.0	36.0	36.0	36.0	36.0
32	34.89925421230475	36.0	36.0	36.0	36.0	36.0
33	34.886997071729574	36.0	36.0	36.0	32.0	36.0
34	34.87309589275081	36.0	36.0	36.0	32.0	36.0
35	34.85329863376376	36.0	36.0	36.0	32.0	36.0
36	34.81651455950605	36.0	36.0	36.0	32.0	36.0
37	34.78326765878335	36.0	36.0	36.0	32.0	36.0
38	34.757727731741014	36.0	36.0	36.0	32.0	36.0
39	34.691855485940266	36.0	36.0	36.0	32.0	36.0
40	34.715748211445955	36.0	36.0	36.0	32.0	36.0
41	34.686881459216536	36.0	36.0	36.0	32.0	36.0
42	34.66034356369641	36.0	36.0	36.0	32.0	36.0
43	34.668699770435616	36.0	36.0	36.0	32.0	36.0
44	34.59534829653423	36.0	36.0	36.0	32.0	36.0
45	34.57660831476969	36.0	36.0	36.0	32.0	36.0
46	34.50481863781257	36.0	36.0	36.0	32.0	36.0
47	34.497490449315144	36.0	36.0	36.0	32.0	36.0
48	34.47356451091241	36.0	36.0	36.0	32.0	36.0
49	34.44298254978574	36.0	36.0	36.0	32.0	36.0
50	34.06078750942021	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	11.0
21	21.0
22	81.0
23	242.0
24	566.0
25	1471.0
26	3219.0
27	6055.0
28	10675.0
29	16738.0
30	25253.0
31	36375.0
32	55085.0
33	93226.0
34	210344.0
35	805203.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.469810401346685	18.16913263045982	12.082563378855573	26.278493589337927
2	15.101928487940821	20.237102950224354	39.29992076007067	25.36104780176416
3	18.76037907136373	24.094786819805087	28.762719799076997	28.382114309754193
4	12.008913708939566	16.337344976826095	36.914079026134594	34.73966228809974
5	13.665920958049737	37.742424494037095	34.055002139068726	14.536652408844436
6	33.790328065122736	36.4137577635331	16.89124964612365	12.904664525220511
7	29.642747845313306	30.540603162025953	20.890754083011682	18.925894909649056
8	26.588821962265403	35.70125473580327	19.378460171014787	18.331463130916543
9	26.956615257846746	14.237894492115496	18.864451050120636	39.94103919991713
10	15.50814191091345	27.512557648145453	32.57499794396189	24.404302496979206
11	36.80811406890411	21.182157715395523	23.433833978217084	18.575894237483283
12	24.51285774046335	23.547785846403006	30.2612985135647	21.678057899568945
13	29.637528675778068	20.2858839652087	25.371964677293214	24.704622681720018
14	22.33211473632518	20.7608283929149	25.76530027226668	31.14175659849324
15	24.055468701193846	29.673588392566952	22.210492270488995	24.060450635750204
16	24.228130122833335	26.446136558278422	25.95742863717707	23.368304681711173
17	22.93050043137227	27.174634203432156	26.456049452422132	23.438815912773443
18	23.37386897955504	25.979743247881093	28.249217518105024	22.397170254458842
19	25.204160468902842	25.100963253092555	25.78910284848039	23.90577342952421
20	25.198981782639535	25.599199090911245	25.97822330718201	23.22359581926721
21	25.293202664306968	25.088785190843677	25.07905855670984	24.538953588139517
22	24.83628809499521	26.22087051003148	25.8138543646096	23.128987030363703
23	23.365626336920066	26.15511870861726	26.30876813960826	24.170486814854414
24	24.339392118175958	25.498772295392452	26.70227866719913	23.45955691923246
25	23.99283548097567	25.6734133872122	26.58455674480948	23.749194387002646
26	22.896569175961694	26.92340844480118	27.03253688027108	23.14748549896605
27	23.70838575600544	26.2613665928334	26.40853228907107	23.621715362090086
28	23.253209842568253	26.505122779048325	26.037208551988538	24.204458826394887
29	23.609899673168762	25.696938161441825	25.999413237890916	24.693748927498504
30	23.297858375363962	26.114266373757072	26.605902159971023	23.98197309090794
31	24.643514623175662	25.331259370789077	25.948466195570347	24.076759810464917
32	23.890304841450654	26.260933653550367	25.89305837054657	23.955703134452406
33	23.23736108881538	26.089483593989087	26.84476188292119	23.82839343427434
34	23.936347719914785	26.259056524097744	26.862192165167592	22.942403590819875
35	24.10064148389015	26.268180121590927	26.708488590570063	22.92268980394886
36	23.10913837046456	26.446085583309415	26.68442765875172	23.76034838747431
37	23.893751423438015	26.209289672799045	25.8390376293747	24.05792127438824
38	23.78011796904716	26.539556164985857	25.91483738728373	23.76548847868325
39	24.723698361485418	25.464667552350225	25.865360283418738	23.94627380274562
40	25.294626074180215	25.52743266678212	26.25859087167248	22.91935038736518
41	24.177895868918835	25.514408173593978	27.03952362877206	23.268172328715124
42	24.51170654785347	26.696670478431894	26.301259147841133	22.4903638258735
43	23.56076548288703	26.128481489653293	26.76011005062044	23.55064297683923
44	23.172722268968702	26.60553070611085	26.17785313450966	24.043893890410786
45	23.40930298764768	26.861862811773452	26.0359498315593	23.692884369019566
46	23.53928750206597	25.705376750541898	26.13051259077433	24.624823156617797
47	24.362562324295897	25.825290319519517	26.194985587815477	23.61716176836911
48	24.0450922089014	26.295748584443107	25.443330275519564	24.215828931135924
49	24.191079014712255	26.21879853604993	25.677962616763306	23.91215983247451
50	23.771008411667996	27.106890735303306	25.228361326097048	23.89373952693165
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	276.0
1	162.0
2	48.0
3	137.5
4	227.0
5	224.0
6	221.0
7	175.5
8	130.0
9	200.5
10	271.0
11	413.0
12	555.0
13	1068.0
14	1581.0
15	2046.5
16	2512.0
17	2530.0
18	2548.0
19	2598.0
20	2648.0
21	3269.5
22	3891.0
23	4995.0
24	6099.0
25	8155.5
26	10212.0
27	14061.0
28	17910.0
29	21405.0
30	24900.0
31	28812.5
32	32725.0
33	39578.5
34	46432.0
35	53573.0
36	60714.0
37	64213.5
38	67713.0
39	67962.0
40	68211.0
41	69482.0
42	70753.0
43	70890.5
44	71028.0
45	74723.0
46	78418.0
47	81551.5
48	84685.0
49	86640.0
50	88595.0
51	85765.5
52	82936.0
53	82031.0
54	81126.0
55	80253.5
56	79381.0
57	75795.5
58	72210.0
59	65476.0
60	58742.0
61	51493.5
62	44245.0
63	38134.5
64	32024.0
65	26899.5
66	21775.0
67	18868.0
68	15961.0
69	14267.5
70	12574.0
71	9881.5
72	7189.0
73	6297.0
74	5405.0
75	4021.0
76	2637.0
77	2362.5
78	2088.0
79	1687.0
80	1286.0
81	981.0
82	676.0
83	605.0
84	534.0
85	418.5
86	303.0
87	203.0
88	103.0
89	62.0
90	21.0
91	19.0
92	17.0
93	13.5
94	10.0
95	8.0
96	6.0
97	9.0
98	12.0
99	8.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03360828867384856
2	0.0043493079460274605
3	3.953916314570419E-4
4	0.0
5	0.0
6	2.3723497887422515E-4
7	0.0
8	0.0
9	0.0
10	7.907832629140837E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	7.907832629140838E-4
17	0.0
18	2.3723497887422515E-4
19	0.0
20	4.744699577484503E-4
21	0.0
22	0.0
23	1.5815665258281673E-4
24	0.0011070965680797173
25	3.1631330516563346E-4
26	3.1631330516563346E-4
27	6.326266103312669E-4
28	0.001660644852119576
29	1.5815665258281673E-4
30	0.0011861748943711257
31	7.907832629140837E-5
32	7.907832629140838E-4
33	1.5815665258281673E-4
34	8.698615892054921E-4
35	7.907832629140837E-5
36	0.0
37	0.0019769581572852096
38	0.0
39	7.117049366226753E-4
40	0.0
41	7.117049366226753E-4
42	0.004981934556358728
43	0.004744699577484503
44	0.005140091208941544
45	0.002293271462450843
46	0.0025305064413250677
47	0.0011070965680797173
48	0.00387483798827901
49	0.003558524683113377
50	0.0012652532206625339
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1264569.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.60515254188956
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.28131634600244	44.70478180553607
2	14.525750176945918	17.025676098105897
3	4.42152381380614	7.773722327271184
4	1.807000576560616	4.2359817773046915
5	0.8718283798918546	2.5546817596955296
6	0.48126733775837743	1.6922847445655278
7	0.31116741744179044	1.2765209775669366
8	0.21862320866138704	1.0249957194238353
9	0.1543018397615571	0.8138594571048229
>10	0.8077509273971504	8.435197505253985
>50	0.06494209540072722	2.6588897115614505
>100	0.04991608335438839	5.861879218427798
>500	0.004340514840161639	1.7714145908570302
>1k	2.7128217751010245E-4	0.17011430732516802
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	1.5815665258281676E-4	0.0	0.0	0.0
6	0.0	1.5815665258281676E-4	0.0	0.0	0.0
7	0.0	1.5815665258281676E-4	0.0	0.0	0.0
8	0.0	1.5815665258281676E-4	0.0	0.0	0.0
9	0.0	1.5815665258281676E-4	0.0	0.0	0.0
10	0.0	1.5815665258281676E-4	0.0	0.0	0.0
11	0.0	1.5815665258281676E-4	0.0	0.0	0.0
12	0.0	2.3723497887422513E-4	0.0	0.0	0.0
13	0.0	2.3723497887422513E-4	0.0	0.0	0.0
14	0.0	2.3723497887422513E-4	0.0	0.0	0.0
15	0.0	2.3723497887422513E-4	0.0	1.5815665258281676E-4	0.0
16	0.0	2.3723497887422513E-4	0.0	2.3723497887422513E-4	0.0
17	0.0	2.3723497887422513E-4	0.0	2.3723497887422513E-4	0.0
18	0.0	2.3723497887422513E-4	0.0	2.3723497887422513E-4	0.0
19	0.0	2.3723497887422513E-4	0.0	2.3723497887422513E-4	0.0
20	0.0	2.3723497887422513E-4	0.0	3.953916314570419E-4	0.0
21	0.0	2.3723497887422513E-4	0.0	8.698615892054921E-4	0.0
22	0.0	2.3723497887422513E-4	0.0	0.0013443315469539425	0.0
23	0.0	2.3723497887422513E-4	0.0	0.0015024881995367593	0.0
24	0.0	2.3723497887422513E-4	0.0	0.002056036483576618	0.0
25	0.0	2.3723497887422513E-4	0.0	0.0024514281150336595	0.0
26	0.0	2.3723497887422513E-4	0.0	0.0032422113779477435	0.0
27	0.0	2.3723497887422513E-4	0.0	0.003637603009404785	0.0
28	0.0	2.3723497887422513E-4	0.0	0.004823777903775911	0.0
29	0.0	2.3723497887422513E-4	0.0	0.006721657734769712	0.0
30	0.0	2.3723497887422513E-4	0.0	0.008935850870929147	0.0
31	0.0	2.3723497887422513E-4	0.0	0.013759628774705058	0.0
32	0.0	2.3723497887422513E-4	0.0	0.019136954962520828	0.0
33	0.0	2.3723497887422513E-4	0.0	0.02380257621371392	0.0
34	0.0	2.3723497887422513E-4	0.0	0.028942667422655464	0.0
35	0.0	2.3723497887422513E-4	0.0	0.0359015601362994	0.0
36	0.0	2.3723497887422513E-4	0.0	0.04365123611285742	0.0
37	0.0	2.3723497887422513E-4	0.0	0.05654100329835699	0.0
38	0.0	2.3723497887422513E-4	0.0	0.07291021684067853	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1495	0.0	27.966679	1
CGTCCGG	45	0.00139852	24.443632	10
TTACTCG	55	1.5923248E-4	24.002998	43
GGTATCA	625	0.0	21.829315	1
TGTAGGA	1575	0.0	21.511248	2
GTAGGAC	1530	0.0	20.84898	3
TAGGACA	535	0.0	20.148863	4
TAGGACG	1445	0.0	19.943974	4
GTATTAC	100	1.1208995E-6	19.804823	1
CTGTAGG	1645	0.0	19.664364	1
TCGATCG	90	1.0012807E-5	19.557226	41
ATCAACG	2105	0.0	19.543291	3
TCAACGC	2110	0.0	19.496983	4
GTCCTAC	1430	0.0	19.389336	1
AACGCAG	2170	0.0	19.059275	6
ATTTCGT	745	0.0	18.900942	42
AGACCGT	70	8.125423E-4	18.856516	6
GGACGTG	1470	0.0	18.856514	6
CAACGCA	2195	0.0	18.741974	5
AGGACGT	1510	0.0	18.648386	5
>>END_MODULE