FastQCFastQC Report
Thu 2 Feb 2017
SRR4062241_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062241_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1966390
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28960.14727495562935125No Hit
GCGCAAGACGGACCAGAGCGAAAGC22860.11625364246156662No Hit
TATCAACGCAGAGTACTTTTTTTTT19820.10079384048942477No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9150.013.8020431
CGGTCCA2850.012.6689110
ACCGTCC1051.9917934E-812.667948
AGCGTTC1001.4412763E-712.3512438
GTATCAA24300.012.2697641
ATCGCCA2900.012.1212916
GACCGTC1103.810419E-812.0921267
GTGTAGG2050.012.0429341
CAAGACG5300.012.0078884
AAGACGG5500.011.9175595
CGGACCA5150.011.8079169
CCGTCGT3250.011.6943799
CGCTTAC658.0268795E-411.69199418
CGCAAGA5000.011.5870312
CGCATCG2800.011.537168513
CGACCAT3300.011.5171910
GTCTAAC752.0837158E-411.3944691
CGTCGTA3350.011.34529110
GCGTTAT1850.011.2918161
TAAGCTG1354.7330104E-911.2586715
Produced by FastQC (version 0.11.5)