##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062241_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1966390
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.280263325179643	32.0	32.0	32.0	32.0	32.0
2	30.985138756808162	32.0	32.0	32.0	32.0	32.0
3	30.91162688988451	32.0	32.0	32.0	32.0	32.0
4	30.998651844242495	32.0	32.0	32.0	32.0	32.0
5	30.930856544225712	32.0	32.0	32.0	32.0	32.0
6	34.50079282339719	36.0	36.0	36.0	32.0	36.0
7	34.297263513341704	36.0	36.0	36.0	32.0	36.0
8	34.214998550643564	36.0	36.0	36.0	32.0	36.0
9	34.5429187495868	36.0	36.0	36.0	32.0	36.0
10	34.15508469835587	36.0	36.0	36.0	32.0	36.0
11	34.29337974664232	36.0	36.0	36.0	32.0	36.0
12	34.24272855333886	36.0	36.0	36.0	32.0	36.0
13	34.24735886573874	36.0	36.0	36.0	32.0	36.0
14	34.23258051556405	36.0	36.0	36.0	32.0	36.0
15	34.366814314556116	36.0	36.0	36.0	32.0	36.0
16	34.37095032012978	36.0	36.0	36.0	32.0	36.0
17	34.16362217057654	36.0	36.0	36.0	32.0	36.0
18	34.308629519067935	36.0	36.0	36.0	32.0	36.0
19	34.30148648030146	36.0	36.0	36.0	32.0	36.0
20	34.13453282410915	36.0	36.0	36.0	32.0	36.0
21	34.10258544846139	36.0	36.0	36.0	32.0	36.0
22	34.22701803813079	36.0	36.0	36.0	32.0	36.0
23	34.0688678237786	36.0	36.0	36.0	32.0	36.0
24	34.0385315222311	36.0	36.0	36.0	32.0	36.0
25	33.740770650786466	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	24.0
5	153.0
6	370.0
7	64.0
8	357.0
9	286.0
10	145.0
11	38.0
12	75.0
13	88.0
14	317.0
15	493.0
16	955.0
17	1211.0
18	1594.0
19	2157.0
20	2915.0
21	3653.0
22	5131.0
23	6734.0
24	9314.0
25	13299.0
26	18810.0
27	24573.0
28	33503.0
29	46083.0
30	62674.0
31	89445.0
32	134966.0
33	204214.0
34	474486.0
35	828260.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.52484623983027	17.5433055970024	11.86122273789218	26.07062542527515
2	16.58310393630552	19.329511433914075	37.42188084034323	26.665503789437178
3	19.325502026311277	22.69646624941962	28.15527611383552	29.822755610433582
4	12.82093274125552	15.543873586537977	35.221950347449855	36.41324332475665
5	14.86262586079807	36.296033652073014	33.36521238826858	15.47612809886034
6	34.75106606010645	35.08543746628808	16.379466511973458	13.784029961632013
7	30.630549455248524	30.222685811619126	20.05714774417603	19.089616988956323
8	28.006237285650577	33.173277619283255	19.063786071080212	19.75669902398596
9	26.812850446234236	14.510440643896985	18.711442322525855	39.965266587342924
10	16.055417730903816	26.822187867900166	31.036363303066228	26.08603109812979
11	36.897988325023285	21.502001082657266	21.878028423063352	19.721982169256098
12	24.200676539616474	23.86439265980486	28.434777782247533	23.500153018331137
13	29.103524198792286	19.72030863408591	25.254458141260233	25.921709025861574
14	23.311335423491162	19.656274597454544	24.934092822965535	32.09829715608876
15	25.093301680270457	27.187363624744183	22.10413429942392	25.615200395561445
16	25.62280461024847	26.036437805585273	23.590991920303818	24.749765663862437
17	23.828435670504756	26.31183942142507	25.11148178358634	24.748243124483835
18	24.557442265504605	25.39925781753145	25.906078127203497	24.13722178976045
19	25.322255439388798	25.143228881661766	25.312583831209057	24.221931847740382
20	25.624542845173842	24.557351965823017	25.0461545039517	24.77195068505144
21	26.319773067492875	24.50513759030294	24.506053790089467	24.669035552114714
22	25.79728353192774	24.453965075145504	25.084950078624956	24.6638013143018
23	24.289748146881408	24.631666595412447	25.381617226419433	25.69696803128671
24	24.372252307463647	25.034930721372206	25.339521521650937	25.253295449513207
25	24.58961238723916	24.632876716512264	25.36490916781317	25.4126017284354
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	133.0
1	133.0
2	354.0
3	575.0
4	575.0
5	575.0
6	1172.5
7	1770.0
8	1770.0
9	1770.0
10	2327.5
11	2885.0
12	2885.0
13	2885.0
14	4162.0
15	5439.0
16	5439.0
17	5439.0
18	9227.5
19	13016.0
20	13016.0
21	13016.0
22	20872.0
23	28728.0
24	28728.0
25	28728.0
26	41335.0
27	53942.0
28	53942.0
29	53942.0
30	67889.0
31	81836.0
32	81836.0
33	81836.0
34	101537.5
35	121239.0
36	121239.0
37	121239.0
38	142213.5
39	163188.0
40	163188.0
41	163188.0
42	186844.5
43	210501.0
44	210501.0
45	210501.0
46	231802.5
47	253104.0
48	253104.0
49	253104.0
50	260054.0
51	267004.0
52	267004.0
53	267004.0
54	249282.0
55	231560.0
56	231560.0
57	231560.0
58	211930.5
59	192301.0
60	192301.0
61	192301.0
62	169228.0
63	146155.0
64	146155.0
65	146155.0
66	120278.0
67	94401.0
68	94401.0
69	94401.0
70	71530.0
71	48659.0
72	48659.0
73	48659.0
74	37116.5
75	25574.0
76	25574.0
77	25574.0
78	20244.0
79	14914.0
80	14914.0
81	14914.0
82	10255.0
83	5596.0
84	5596.0
85	5596.0
86	3989.0
87	2382.0
88	2382.0
89	2382.0
90	1526.5
91	671.0
92	671.0
93	671.0
94	415.0
95	159.0
96	159.0
97	159.0
98	408.5
99	658.0
100	658.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.136737880074655E-4
2	1.5256383525139977E-4
3	4.5769150575419935E-4
4	0.010526904632346583
5	0.03188584156754255
6	0.06153408021806457
7	0.10211606039493691
8	0.10547246477046772
9	0.1163044970733171
10	0.1292724230696861
11	0.1374600155615112
12	0.13084891603395055
13	0.1276959301054216
14	0.11513484100305638
15	0.13217113593946267
16	0.11854209999033762
17	0.12164423130711609
18	0.11101561745126858
19	0.0954541062556258
20	0.09118231886858659
21	0.08914813439856793
22	0.10176007811268364
23	0.08106225113024375
24	0.0908771911980838
25	0.08762249604605393
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1966390.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.9224307397948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.00588203228425	34.02774463810182
2	15.4985216718972	14.854536627814047
3	5.819474741522016	8.36650125727722
4	2.6269839764058074	5.035658306554321
5	1.3869181079965063	3.32322434861149
6	0.8234176748654454	2.367610589619731
7	0.5530175375445168	1.8551361248608278
8	0.39188664657464	1.50241285426589
9	0.2766762066576366	1.1933096715809766
>10	1.4368669329651302	12.366940131498636
>50	0.0984267563420892	3.247614574837461
>100	0.0705444859646441	6.9105993294778445
>500	0.007978898817896813	2.630132588014337
>1k	0.0034043301623026398	2.318578957485433
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2896	0.14727495562935125	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	2286	0.11625364246156662	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1982	0.10079384048942477	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	5.085461175046659E-5	0.0
8	0.0	0.0	0.0	5.085461175046659E-5	0.0
9	0.0	0.0	0.0	5.085461175046659E-5	0.0
10	0.0	0.0	0.0	2.0341844700186637E-4	0.0
11	0.0	0.0	0.0	2.0341844700186637E-4	0.0
12	0.0	0.0	0.0	2.0341844700186637E-4	0.0
13	0.0	0.0	0.0	2.0341844700186637E-4	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	915	0.0	13.802043	1
CGGTCCA	285	0.0	12.66891	10
ACCGTCC	105	1.9917934E-8	12.66794	8
AGCGTTC	100	1.4412763E-7	12.351243	8
GTATCAA	2430	0.0	12.269764	1
ATCGCCA	290	0.0	12.12129	16
GACCGTC	110	3.810419E-8	12.092126	7
GTGTAGG	205	0.0	12.042934	1
CAAGACG	530	0.0	12.007888	4
AAGACGG	550	0.0	11.917559	5
CGGACCA	515	0.0	11.807916	9
CCGTCGT	325	0.0	11.694379	9
CGCTTAC	65	8.0268795E-4	11.691994	18
CGCAAGA	500	0.0	11.587031	2
CGCATCG	280	0.0	11.5371685	13
CGACCAT	330	0.0	11.51719	10
GTCTAAC	75	2.0837158E-4	11.394469	1
CGTCGTA	335	0.0	11.345291	10
GCGTTAT	185	0.0	11.291816	1
TAAGCTG	135	4.7330104E-9	11.258671	5
>>END_MODULE